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The map label for this gene is hsdS [H]

Identifier: 30065580

GI number: 30065580

Start: 4526799

End: 4528454

Strand: Reverse

Name: hsdS [H]

Synonym: S4635

Alternate gene names: 30065580

Gene position: 4528454-4526799 (Counterclockwise)

Preceding gene: 30065583

Following gene: 30065578

Centisome position: 98.46

GC content: 40.58

Gene sequence:

>1656_bases
ATGAGTGTGGAAAAGCTGATCGTTGATCATATGGAAACCTGGACCTCGGCGTTGCAAACCCGTTCCACCGCCGGGCGCGG
CAGCTCCGGTAAGATTGATTTGTATGGTATTAAGAAATTGCGTGAGCTGATTCTGGAACTGGCGGTGCGCGGTAAACTGG
TGCCGCAGGATCCGAATGATGAACCGGCGTCGGACCTGCTGAAGCGTATTGCGGCGGAAAAAGCAGAGCTGGTGAAGCAA
GGCAAAATTAAAAAGCAAAAACCACTGCCAGAAATTAGTGAGGAAGAGAAGCCGTTTGAATTGCCGGAGGGATGGGAGTG
GGTGCACCTCCCTGATATTTATTGCTCAATTTCTGAGTCATCTAGAAAAATAAAATCCTCCGAAATACTACCTGAGGGAA
AATATCCTGTTATCGAGCAAAGCCAAGAATTTATTTCTGGTTATTGCAACAATGAATGTCTGTTAATAAAGTTAAATAAT
CCAGTTATAGTTTTTGGTGATCACACAAGGAATATTAAATTTATTGATTTTGATTTTGTTGTCGGCGCTGACGGTGTGAA
GATTTTATCACCTATATTAATTTGTGAGCGTTTCTTTTTTTGGCAGTTACGATCATTTAAATTAGATGTAAGAGGATACG
CGAGACATTTTAAAGTATTAAATAGCTGCTTATTTGCATTACCCCCCATTGCAGAACAAGAGCGAATAGTTGAAAAAGTT
AGCTCTTTGATGTCCCTCTGCGACCAACTAGAACAGCAATCCCTGACCAGTCTGGACGCACATCAGCAACTGGTTGAAAC
CCTGTTGGGAACGCTTACAGACAGCCAAAACACCGCGGAACTGGCTGAAAACTGGGCGCGTATTAGCGAGCATTTCGACA
CCCTATTTACCACCGAAGCCAGCGTGGATGCGTTAAAACAGACCATTCTGCAACTGGCCGTAATGGGCAAACTTGTGCCG
CAGGATCCGAATGACGAACCCGCCTCTGAACTGCTCAAACGTATTGCGCAGGAAAAAGCTCAATTGGTGAAAGAAGGGAA
AATACAAAAACCACTGCCGCCAATTAGCGATGAGGAAAAACCGTTTGAACTTCCGGAAGGGTGGGAATGGTGTCGTATTG
GTAATATAGTTAATATTAAATCTGAATTAGTATCACCAAAAGATTATTTAAATTTATATCAAGTAGCACCTGATATTATT
GAAAAGGGCACTGGGAGAGTGATATCAAAGAGAACAGTGAAAGAATCAGGAGTCAAAGGGCCAAATAGTCGCTTTTATAA
AGGACAAATTGTTTATTCTAAAATCCGCCCGTCATTATCCAAAGTTTTTTTAGCAGAGTATAATGGTCTTTGCAGTGCTG
ATATGTATCCATTAGACTGTTACATTAATCCAAATTACTTATTGAAATATATCCTTTCCATTCCATTTCTTATGCAAGTC
AAAAAAGCAGAGAACAGAATAAAAATGCCAAAACTTAATTCTGACAGTTTTTACAATATTATTGTTGCGATTCCCCCCTA
TAATGAACAACAAGCAATATTTGATAAGATAAATTCTATTGAAGCAGTATGCAATGGTCTTATTTCTTATATTGGGATTT
ATCATAAAACCCAACTGCATCTTGCAGACGCACTCACTGACGCAGCGATAAACTAA

Upstream 100 bases:

>100_bases
TGTTAGCGCAGTATGCAAAACAGCAGGCGGAGATCCAGACGCTGCGTAATCAACTGCGCGATATTCTTGGCGCTGCGCTG
TCTGGCAAGGAGGTTAACTA

Downstream 100 bases:

>100_bases
TTCCCTCGCCCTCACAAAACCAATAAAGCGGCTAAATAGCCGCTTTATTCACATCACCAAAATTTATATTTCACGCTAAT
TTTCATCTTATTTTCATTAA

Product: hypothetical protein

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQ
GKIKKQKPLPEISEEEKPFELPEGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKV
SSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVP
QDPNDEPASELLKRIAQEKAQLVKEGKIQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDII
EKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQV
KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN

Sequences:

>Translated_551_residues
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQ
GKIKKQKPLPEISEEEKPFELPEGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKV
SSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVP
QDPNDEPASELLKRIAQEKAQLVKEGKIQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDII
EKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQV
KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN
>Mature_550_residues
SVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQG
KIKKQKPLPEISEEEKPFELPEGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNNP
VIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKVS
SLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQ
DPNDEPASELLKRIAQEKAQLVKEGKIQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIE
KGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQVK
KAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 62349; Mature: 62217

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPND
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELPEGWEWVHLPDIYCSISES
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHEEECCHH
SRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNNPVIVFGDHTRNIKFIDFDFV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCEEEEEEEEE
VGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKV
ECCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHH
SVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIQKPLPPISDEEK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKRTVKESGVKG
CCCCCCCCCHHCCCCEEECHHHHCCCHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCC
PNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQV
CCCCEEECEEEHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLH
HHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADALTDAAIN
HHHHHHHHCCC
>Mature Secondary Structure 
SVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPND
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELPEGWEWVHLPDIYCSISES
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHEEECCHH
SRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNNPVIVFGDHTRNIKFIDFDFV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCEEEEEEEEE
VGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVEKV
ECCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLFTTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHH
SVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIQKPLPPISDEEK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKRTVKESGVKG
CCCCCCCCCHHCCCCEEECHHHHCCCHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCC
PNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQV
CCCCEEECEEEHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLH
HHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADALTDAAIN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]