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The map label for this gene is cadB

Identifier: 30065507

GI number: 30065507

Start: 4456077

End: 4457129

Strand: Reverse

Name: cadB

Synonym: S4552

Alternate gene names: 30065507

Gene position: 4457129-4456077 (Counterclockwise)

Preceding gene: 30065510

Following gene: 30065505

Centisome position: 96.91

GC content: 48.91

Gene sequence:

>1053_bases
ATGAGTTCTGCCAAGAAGATCGGGCTATTTGCCTGTACCGGTGTTGTTGCCGGTAATATGATGGGGAGCGGTATTGCATT
ATTACCTGCGAACCTAGCAAGTATCGGTGGTATTGCTATCTGGGGTTGGATTATCTCTATTATTGGTGCAATGTCGCTGG
CGTATGTATATGCCCGACTGGCAACAAAAAACCCGCAACAAGGTGGCCCAATTGCTTATGCCGGAGAAATTTCCCCTGCA
TTTGGTTTTCAGACAGGTGTTCTTTATTACCATGCTAACTGGATTGGTAACCTGGCGATTGGTATTACCGCTGTATCTTA
TCTTTCCACCTTCTTCCCAGTATTAAATGATCCTGTTCCGGCGGGTATCGCCTGTATTGCTATCGTCTGGGTATTTACCT
TTGTAAATATGCTCGGCGGTACCTGGGTAAGCCGTTTAACCACTATTGGTCTGGTGCTGGTTCTTATTCCTGTGGTGATG
ACTGCTATTGTTGGCTGGCATTGGTTTGATGCGGCAACTTATGCAGCTAACTGGAATACTGCGGATACCACTGATGGTCA
TGCGATCATTAAAAGTATTCTGCTCTGCCTGTGGGCCTTCGTGGGTGTTGAATCCGCAGCAGTAAGTACTGGTATGGTTA
AAAACCCGAAACGTACCGTTCCGCTGGCAACCATGCTGGGTACTGGTTTAGCAGGTATTGTTTATATCGCTGCGACTCAG
GTGCTTTCCGGTATGTATCCGTCTTCTGTAATGGCGGCTTCCGGTGCTCCGTTTGCAATCAGTGCTTCAACTATCCTCGG
TAACTGGGCTGCGCCGCTGGTTTCTGCATTCACCGCCTTTGCGTGCCTGACTTCTCTGGGCTCCTGGATGATGTTGGTAG
GCCAGGCAGGTGTACGTGCCGCTAACGACGGTAACTTCCCGAAAGTTTATGGTGAAGTCGACAGCAACGGTATTCCGAAA
AAAGGTCTGCTGCTGGCTGCAGTGAAAATGACTGCCCTGATGATCCTCATCACTCTGATGAACTCTGCCGGTGGTAAAGC
ATCTGACCTGTGA

Upstream 100 bases:

>100_bases
TTTTTTCGTGAGTAATCTTATCGCCAGTTTGGTCTGGTCAGGAAATAGTTATACATCATGACCCGGACTCCAAATTCAAA
AATGAAATTAGGAGAAGAGC

Downstream 100 bases:

>100_bases
GGTGTACTGGCAATAGCGGACACTACCATTTGTTCTTTTTTTAAGCAGCCATCTGATGATATTTTTCCCTGAAGGCTGCC
GGGGAGATATTCCCCAGACG

Product: transport of lysine/cadaverine

Products: cadaverine [Cytoplasm]; L-lysine [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPA
FGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPK
KGLLLAAVKMTALMILITLMNSAGGKASDL

Sequences:

>Translated_350_residues
MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPA
FGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPK
KGLLLAAVKMTALMILITLMNSAGGKASDL
>Mature_349_residues
SSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAF
GFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMT
AIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQV
LSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKK
GLLLAAVKMTALMILITLMNSAGGKASDL

Specific function: Probable cadaverine/lysine antiporter or part of it

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Escherichia coli, GI1790575, Length=350, Percent_Identity=100, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=331, Percent_Identity=44.7129909365559, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1786908, Length=350, Percent_Identity=34, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787890, Length=352, Percent_Identity=24.7159090909091, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082250, Length=324, Percent_Identity=26.5432098765432, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982612, Length=338, Percent_Identity=24.2603550295858, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71982608, Length=338, Percent_Identity=24.2603550295858, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI221331183, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17647653, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24664379, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CADB_ECO57 (P0AAF0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91268
- RefSeq:   NP_290766.1
- RefSeq:   NP_313141.1
- ProteinModelPortal:   P0AAF0
- SMR:   P0AAF0
- EnsemblBacteria:   EBESCT00000024488
- EnsemblBacteria:   EBESCT00000057772
- GeneID:   914165
- GeneID:   959991
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5735
- KEGG:   ecs:ECs5114
- GeneTree:   EBGT00050000009006
- HOGENOM:   HBG586921
- OMA:   VGKFIKI
- ProtClustDB:   PRK10435
- BioCyc:   ECOL83334:ECS5114-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR00905

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 36181; Mature: 36050

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16905b34)-; HASH(0x15b52434)-; HASH(0x1684da54)-; HASH(0x16c09f94)-; HASH(0x16d18838)-; HASH(0x14fd3744)-; HASH(0x16f6eb88)-; HASH(0x16ea6d38)-; HASH(0x16ca03ac)-; HASH(0x16ebee9c)-; HASH(0x148aee40)-; HASH(0x1691c194)-; HASH(0x1708f3c0)-; HASH(0x16f7c63c)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
SSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARL
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVP
HCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRA
HHHCCCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDL
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cadaverine [Cytoplasm]; Proton [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: cadaverine [Cytoplasm] + Proton [Periplasm] + L-lysine [Periplasm] = cadaverine [Cytoplasm] + L-lysine [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796