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The map label for this gene is yjfG

Identifier: 30065479

GI number: 30065479

Start: 4422102

End: 4423475

Strand: Reverse

Name: yjfG

Synonym: S4519

Alternate gene names: 30065479

Gene position: 4423475-4422102 (Counterclockwise)

Preceding gene: 30065481

Following gene: 30065477

Centisome position: 96.18

GC content: 55.31

Gene sequence:

>1374_bases
ATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGT
AACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGTTACG
ATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCG
GTACTGGAAAAAAACATCCCTTATATGTCAGGTCCACAGTGGCTGCACGATTTTGTGCTGCGCGACCGCTGGGTGCTGGC
CGTTGCCGGTACACACGGCAAAACCACCACCGCGGGAATGGCGACCTGGATTCTGGAACAGTGTGGTTACAAACCGGGAT
TTGTGATCGGCGGTGTGCCGGGGAACTTTGAGGTTTCGGCGCGTCTGGGCGAAAGCGACTTCTTTGTTATCGAAGCGGAT
GAGTACGACTGCGCCTTCTTCGACAAACGCTCTAAATTTGTCCATTACTGCCCGCGTACGCTGATCCTCAACAACCTTGA
GTTCGATCACGCCGATATCTTTGACGACCTGAAAGCGATCCAGAAACAGTTCCACCATCTGGTGCGTATCGTTCCGGGGC
AGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTG
GTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAA
AGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGCCTGATGGCGATTGCAGCGGCTCGCCATG
TTGGTGTAGCGCCGGCAGATGCCGCTAACGCGCTGGGTTCGTTTATTAATGCTCGTCGCCGTCTGGAGTTGCGTGGTGAA
GCGAATGGCGTCACGGTATATGACGATTTTGCCCATCACCCGACGGCGATTCTGGCAACGCTGGCGGCGCTGCGTGGCAA
AGTTGGTGGTACGGCGCGCATTATTGCTGTGCTGGAACCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATC
TGGCACCTTCATTAGGTCGTGCTGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCA
GAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGG
CGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGTCTGGCGAAGAAGGCGG
AAGCTGCGCAGTAA

Upstream 100 bases:

>100_bases
GTGAATCGCGCCAGCAAATTGCGGATTATCCTGAAATGCGTTTCTCACTTGCCCGACATATGCGTAAAATGAGCGGCAGA
TTAAAAAAGGATAGTGACGT

Downstream 100 bases:

>100_bases
TTCGGCTTCAGCCTGATATAGCATTGCCGGATAAGGCGTTTACGCCGCATCCGGCAAACCATACTCACATCAACAACGAA
AATTACCCTTCATTCTCCGC

Product: putative ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEA
VLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQEL
VGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVA
EACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ

Sequences:

>Translated_457_residues
MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEA
VLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQEL
VGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVA
EACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ
>Mature_457_residues
MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEA
VLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQEL
VGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVA
EACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=457, Percent_Identity=99.781181619256, Blast_Score=942, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=407, Percent_Identity=28.5012285012285, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49894; Mature: 49894

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCC
QPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHCCCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHCCCCHHHH
VGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPAD
CCCCCCCCHHHCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCH
AANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVAEACVQPAHWSGDVDTLADMV
CCCCEEEECCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCC
QPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEADEYDCAFFDKRSKFVHYCPRT
HHHHHHHCCCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCEEEHHCCCHHHHHCCHH
LILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQEL
HEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHCCCCHHHH
VGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPAD
CCCCCCCCHHHCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCH
AANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEP
HHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
RSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVAEACVQPAHWSGDVDTLADMV
CCCCEEEECCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]