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The map label for this gene is pmbA

Identifier: 30065477

GI number: 30065477

Start: 4420022

End: 4421374

Strand: Reverse

Name: pmbA

Synonym: S4517

Alternate gene names: 30065477

Gene position: 4421374-4420022 (Counterclockwise)

Preceding gene: 30065479

Following gene: 30065471

Centisome position: 96.13

GC content: 54.62

Gene sequence:

>1353_bases
ATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGA
GTTGGCCTCAGGCAAATCGGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTG
AAGTGGAGAATGTTGAATTCAATAGTGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGTAAAGGTAGCGCATCG
TCTACCGATTTAAGCCCGCAGGCCATTGCCCGCACCGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGATCC
CTGTGCCGGCGTGGCAGACAAAGAGCTGCTGGCCTTTGACGCACCGAATCTCGACTTGTTCCACCCTGCGGAAGTTTCCC
CGGATGAAGCCATTGAACTGGCGGCCCGCGCAGAACAGGCGGCATTGCAGGCGGACAAACGCATCACCAATACCGAAGGT
GGCAGCTTTAACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCA
TTCGCTCTTCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGCCGCGCGATGA
GCGATCTGCAAACGCCAGAGTGGGTCGGGGCCGACTGTGCTCGCCGTACTTTATCGCGCCTGTCACCGCGTAAACTCTCC
ACCATGAAAGCGCCGGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGCGGGTGG
CTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCTGACCATTGAAGAGC
ATCCGCATCTGCTGAAAGGGCTGGCGTCGACGCCATTCGACAGCGAAGGTGTGCGCACCGAGCGTCGCGATATTATTAAA
GATGGCATCCTGACTCAGTGGCTGCTGACCAGCTACTCGGCGCGGAAACTGGGGCTGAAAAGCACCGGACATGCGGGCGG
TATTCACAACTGGCGGATTGCCGGACAAGGGCTAAGCTTCGAACAAATGCTCAAAGAGATGGGCACCGGGCTGGTGGTGA
CGGAGCTGATGGGCCAGGGCGTGAGTGCCATTACCGGTGATTATTCCCGTGGTGCAGCGGGCTTCTGGGTAGAGAATGGC
GAAATTCAGTATCCCGTTAGTGAAATCACCATCGCAGGTAATTTAAAAGATATGTGGCGCAATATTGTCACCGTCGGTAA
CGATATTGAAACACGCAGTAATATACAGTGTGGTTCTGTGCTGTTGCCGGAGATGAAAATCGCCGGGCAGTAA

Upstream 100 bases:

>100_bases
TAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACT
TCAGAGAAACTCTCTACATT

Downstream 100 bases:

>100_bases
AAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATG
AGGGCCGGGGAGCGTACTCG

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
GSFNSHYGVKVFGNSHGMLQGYCSTRHSLFSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIK
DGILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENG
EIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ

Sequences:

>Translated_450_residues
MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
GSFNSHYGVKVFGNSHGMLQGYCSTRHSLFSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIK
DGILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENG
EIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
>Mature_449_residues
ALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASS
TDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEGG
SFNSHYGVKVFGNSHGMLQGYCSTRHSLFSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLST
MKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKD
GILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENGE
IQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=450, Percent_Identity=99.5555555555556, Blast_Score=924, Evalue=0.0,
Organism=Escherichia coli, GI1789640, Length=408, Percent_Identity=20.0980392156863, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48429; Mature: 48298

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEE
NSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEGGSFNSHYGVKVFGNSHGMLQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCH
GYCSTRHSLFSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLS
HHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHH
LASTPFDSEGVRTERRDIIKDGILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEECCCCCH
EQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMWR
HHHHHHHCCCEEEHHHHCCCCHHHCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHH
NIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
HHHHCCCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure 
ALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEE
NSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEGGSFNSHYGVKVFGNSHGMLQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCH
GYCSTRHSLFSCVIAEENGDMERDYAYTIGRAMSDLQTPEWVGADCARRTLSRLSPRKLS
HHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFLLDSLGKQILPDWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHH
LASTPFDSEGVRTERRDIIKDGILTQWLLTSYSARKLGLKSTGHAGGIHNWRIAGQGLSF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEECCCCCH
EQMLKEMGTGLVVTELMGQGVSAITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMWR
HHHHHHHCCCEEEHHHHCCCCHHHCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHH
NIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
HHHHCCCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]