Click here to switch to the map view.

The map label for this gene is intB [H]

Identifier: 30065448

GI number: 30065448

Start: 4386934

End: 4388145

Strand: Reverse

Name: intB [H]

Synonym: S4839

Alternate gene names: 30065448

Gene position: 4388145-4386934 (Counterclockwise)

Preceding gene: 30065451

Following gene: 30065440

Centisome position: 95.41

GC content: 51.49

Gene sequence:

>1212_bases
ATGGCTCTGAGTGATGTGAAGGTTCGTTCGGCTAAGCCTGAAGCAAAAGCCTATAAGCTGACCGACGGTGAAGGCATGGT
TTTGCTGGTTCACCCTAACGGCTCCAAATACTGGCGGCTACGTTATCGCTTTGGCGGCAAAGAGAAGATGCTGGCATTGG
GGAAATACCCCGAAGTGTCGTTGGCGGATGCCAGGGCACGCCGGGATGAGGCTCGTAAGCTTTTAGCTAATGGTGTCGAT
CCCAGTGAAAACAAGAAAGCTGTTAAGGTAGAACAGGAACAAGAGGCGATAACGTTTGAAGTAGTCGCCAGAGACTGGCA
CGCCAGCAATCAGAAGTGGTCGGCATCGCATAGCGCTCGTGTTTTGAAAAGTCTGGAGGATAATCTCTTTACTGCTATCG
GTAAGCGGAACATTGCGGAACTGAAGACGCGGGATCTTCTTGTACCCATTAAAGCAGTCGAGTCATCCGGGCGGCTCGAA
GTTGCCGCCCGTTTACAGCAGCGTACTACCGCGATTATGCGCTTTGCCGTTCAGAGCGGCTTAATCGACTACAACCCCGC
GCAAGAGATTGCAGGTGCGGTTGCTACGGCGAAAAGACAGCACCGTGCAGCTCTTGAACTGAACCGTATACCTGAATTAC
TTCATCGCATAGATCACTATTCTGGCAGACCGTTAACTCGACTGGCGGTTGAACTCACTTTATTGGTTTTCATCCGTTCG
AGCGAGCTGCGTTTTGCCCGCTGGTCAGAAATAGATTTTGAAACGGCTATGTGGACAATCCCAGGTGAGCGTGAGCAACT
GGAAGGAGTCAAGCATTCTCAGCGTGGCTCGAAGATGCGGACACCACACCTTGTACCTTTGTCGCGGCAGGCCCTAAGTA
TTTTGGAAAAGATCAAAAGCATGAGCGGAAATCGAGAGCTGATTTTTGTGGGCGATCACGATCCACGTAAGCCGATGAGT
GAGAATACGGTGAACAAGGCTTTGCGCGTAATGGGGTATGACACGAAGGTCGAGGTTTGTGGGCATGGCTTCAGGACGAT
GGCTTGTAGTTCGTTGATTGAGTCGGGATTATGGTCGAGGGATGCGGTAGAGCGGCAGATGAGTCACCAGGAGCGTAGCT
CTGTGCGAGCAGCTTACATCCATAAGGCGGAACATCTTGGGGAGCGTAGGTTGATGTTTGAAGTGGTCAACAAAAACTGG
CCACCGAGTTAG

Upstream 100 bases:

>100_bases
GATTCAATCTACATCAACTTACTGATGCGGGTACAACTGCGGGTATTTCCCGGTTCGATAATGTTTGTACCCACACAGAA
CCCTTGAAAGGATACTCATC

Downstream 100 bases:

>100_bases
AGTTTTTCCAGTATCGATTTTCCGATTCGTTTGGGGGTAACCCACCGTTATATTCGTGCGGTCTTAGTGCGCTGTAATAT
CCAACGATATAGTCCGTTAT

Product: putative P4-type integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLALGKYPEVSLADARARRDEARKLLANGVD
PSENKKAVKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
SELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMS
ENTVNKALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLMFEVVNKNW
PPS

Sequences:

>Translated_403_residues
MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLALGKYPEVSLADARARRDEARKLLANGVD
PSENKKAVKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
SELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMS
ENTVNKALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLMFEVVNKNW
PPS
>Mature_402_residues
ALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLALGKYPEVSLADARARRDEARKLLANGVDP
SENKKAVKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEV
AARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRSS
ELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSE
NTVNKALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLMFEVVNKNWP
PS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=396, Percent_Identity=35.3535353535354, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI145693166, Length=396, Percent_Identity=35.6060606060606, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788690, Length=390, Percent_Identity=31.7948717948718, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45663; Mature: 45532

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLALGKYPEVS
CCCCCCEECCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCHHEEEECCCCCCC
LADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVVARDWHASNQKWSASHSAR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHEEEEEEEHHHCCCCCCCCCHHHHH
VLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEVAARLQQRTTAIMRFAVQSG
HHHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEC
SELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLSRQALSILEKIKS
CCCCEEECCCCCCCCEEEECCCCHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
MSGNRELIFVGDHDPRKPMSENTVNKALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSR
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCH
DAVERQMSHQERSSVRAAYIHKAEHLGERRLMFEVVNKNWPPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLALGKYPEVS
CCCCCEECCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCHHEEEECCCCCCC
LADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVVARDWHASNQKWSASHSAR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHEEEEEEEHHHCCCCCCCCCHHHHH
VLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEVAARLQQRTTAIMRFAVQSG
HHHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEC
SELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLSRQALSILEKIKS
CCCCEEECCCCCCCCEEEECCCCHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
MSGNRELIFVGDHDPRKPMSENTVNKALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSR
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCH
DAVERQMSHQERSSVRAAYIHKAEHLGERRLMFEVVNKNWPPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]