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The map label for this gene is iraD

Identifier: 30065417

GI number: 30065417

Start: 4357369

End: 4357770

Strand: Reverse

Name: iraD

Synonym: S4448

Alternate gene names: 30065417

Gene position: 4357770-4357369 (Counterclockwise)

Preceding gene: 30065418

Following gene: 161486372

Centisome position: 94.75

GC content: 48.76

Gene sequence:

>402_bases
GTGCGCAAAATGATGCGCCAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATTGCTGCGTAAATC
GGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGT
GGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAG
CCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTA
CCACGACGATGAACCGCTAACGTTTGATCTGGTCTGGGATAATGGCGGTTGGCGCAGTGCGACGCTTGAGAATGTCAGCT
AA

Upstream 100 bases:

>100_bases
ATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCA
TATGTTGCTAAACGTCAGGA

Downstream 100 bases:

>100_bases
GCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACTGGATTCATGCGGGTATTCATCCGGTATGCGTAG
GCCTGAATCGGGATCACCTG

Product: DNA replication/recombination/repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHE
PRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS

Sequences:

>Translated_133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHE
PRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS
>Mature_133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHE
PRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS

Specific function: Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS during oxidative stress. Its effect on RpoS stability is due to its interaction with RssB, which probably blocks the interaction of Rss

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GpW/Gp25 family. IraD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082422, Length=130, Percent_Identity=99.2307692307692, Blast_Score=267, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007048 [H]

Pfam domain/function: PF04965 GPW_gp25 [H]

EC number: NA

Molecular weight: Translated: 15189; Mature: 15189

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCC
DKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEEEEEEEECCCCEEEEEEEC
NGGWRSATLENVS
CCCCCCCCCCCCC
>Mature Secondary Structure
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCC
DKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEEEEEEEECCCCEEEEEEEC
NGGWRSATLENVS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA