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The map label for this gene is gntR

Identifier: 30065279

GI number: 30065279

Start: 4221348

End: 4222286

Strand: Direct

Name: gntR

Synonym: S4302

Alternate gene names: 30065279

Gene position: 4221348-4222286 (Clockwise)

Preceding gene: 30065278

Following gene: 161486383

Centisome position: 91.78

GC content: 54.95

Gene sequence:

>939_bases
ATGACGGTCAGCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGA
ACTGGGCTATATTCCCAATCGTGCGCCCGATATCCTCTCTAACGCTACCAGCCGGGCGATTGGCGTCCTGTTACCTTCTC
TCACCAACCAGGTTTTCGCGGAAGTATTACGCGGAATCGAAAGCGTCACCGACGCGCACGGTTATCAGACCATGCTGGCG
CACTACGGTTATAAACCGGAAATGGAGCAAGAACGTCTCGAATCCATGCTCTCCTGGAATATCGACGGCCTGATCCTCAC
CGAACGTACCCACACGCCGCGCACCTTAAAGATGATTGAAGTGGCGGGGATCCCGGTGGTGGAGTTGATGGACAGCCAGT
CGCCGTGCCTCGATATCGCCGTCGGTTTTGATAACTTTGAAGCGGCACGCCAGATGACTACCGCCATTATTGCTCGTGGG
CATCGCCACATTGCCTATCTCGGCGCACGTCTCGACGAACGTACTATCATCAAACAGAAGGGATACGAACAGGCGATGCT
GGATGCAGGCCTGGTGCCATATAGCGTGATGGTTGAGCAATCTTCTTCTTACTCTTCCGGTATTGAACTGATTCGCCAGG
CGCGGCGGGAATATCCGCAGCTGGATGGCGTGTTCTGTACTAATGATGATCTGGCGGTCGGCGCGGCGTTTGAATGTCAG
CGTCTGGGGTTAAAAGTTCCTGACGATATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACG
ACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTATTCGTGGCGAAT
CTGTGACACCGAAAATGTTAGATTTAGGTTTCACCTTGTCACCGGGCGGATCTATTTAA

Upstream 100 bases:

>100_bases
GTGTTAAACTAAGAGAATCTATCTCTTTTGTACCTTCAGGACGATGAAAAAGAAAAGACCCGTACTTCAGGATGTGGCTG
ACCGTGTAGGCGTGACCAAA

Downstream 100 bases:

>100_bases
GCCCACAAATTTGAAGTAGCTCACACTTATACACTTAAGGCACGGATGGATATTGCTTCTGATATTGTCCGGCTGGACAA
TGTTGCCGATAACAGTTACC

Product: regulator of gluconate (gnt) operon

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLA
HYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARG
HRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQ
RLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI

Sequences:

>Translated_312_residues
MTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLA
HYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARG
HRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQ
RLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI
>Mature_311_residues
TVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAH
YGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARGH
RHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQR
LGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=99.6794871794872, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=46.0063897763578, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1790369, Length=299, Percent_Identity=29.4314381270903, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=27.4760383386581, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787948, Length=306, Percent_Identity=27.1241830065359, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788474, Length=294, Percent_Identity=25.1700680272109, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787580, Length=302, Percent_Identity=24.8344370860927, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789068, Length=306, Percent_Identity=24.1830065359477, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786540, Length=308, Percent_Identity=25.3246753246753, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789202, Length=280, Percent_Identity=22.1428571428571, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=22.1518987341772, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34272; Mature: 34141

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFA
CCHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
VAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQK
ECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHEEHHHCCCCHHHHHHHC
GYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQ
CHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCHHHHH
RLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKML
HCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCHHH
DLGFTLSPGGSI
HCCEEECCCCCC
>Mature Secondary Structure 
TVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFA
CHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
VAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQK
ECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHEEHHHCCCCHHHHHHHC
GYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQ
CHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCHHHHH
RLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKML
HCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCHHH
DLGFTLSPGGSI
HCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]