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The map label for this gene is yhhU

Identifier: 30065248

GI number: 30065248

Start: 4189923

End: 4190510

Strand: Reverse

Name: yhhU

Synonym: S4270

Alternate gene names: 30065248

Gene position: 4190510-4189923 (Counterclockwise)

Preceding gene: 30065249

Following gene: 30065247

Centisome position: 91.11

GC content: 56.63

Gene sequence:

>588_bases
ATGTATCGGATAGTTCTGGGGAAAGTTTCGACCTTAAGCGCAGCTCCACTGCCACCGGGTTTACGCGAGCAAGCACCGCA
AGGTCCACGACGCGAACGCTGGCTGGCGGGGCGTACATTGCTTTCGCACACGCTTTCCCCGCTACCGGAGATCATCTATG
GCGAACAAGGCAAACCTGCATTCGCGCCGGAAACGCCGCTATGGTTCAACTTAAGCCATAGCGGTGACGATATCGCTCTG
CTGTTGAGCGATGAAGGCGAAGTCGGCTGCGATATCGAAGTGATTCGCCCGCGCGTCAACTGGCGCTGGCTGGCGAACGC
CGTGTTCAGCCTCGGGGAACACGCTGAAATGGACGCCGTGCATCCTGATCAGCAACTGGAAATGTTCTGGCGCATCTGGA
CGCGCAAAGAAGCCATCGTTAAACAGCGTGGCGGCAGCGCCTGGCAAATCGTCAGCGTAGATAGCACCTATCACTCCTCG
CTGTCAGTCAGCCATTGTCAGCTAGAAAATTTAAGCCTTGCGATCTGCACCCCCACCCCCTTTACGCTCACCGCCGACAG
TGTGCAATGGATCGATTCAGTTAACTGA

Upstream 100 bases:

>100_bases
GATTTTACTGGGGCCGGGCAGACCGAAAAGTCGGTTACCGGGATGAGGCGACAAGTGATACGATACGCACTTTCATTTTC
CATTAAACGTTGGCCCTGAT

Downstream 100 bases:

>100_bases
TCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTC
CGCCATCTATTTTAATCCAT

Product: holo-(acyl carrier protein) synthase 2

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRVNWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Sequences:

>Translated_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRVNWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN
>Mature_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRVNWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Specific function: May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Escherichia coli, GI1789886, Length=195, Percent_Identity=98.4615384615385, Blast_Score=395, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 21796; Mature: 21796

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCCHHHCCCC
>Mature Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]