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The map label for this gene is melA

Identifier: 30064688

GI number: 30064688

Start: 3505525

End: 3506880

Strand: Direct

Name: melA

Synonym: S3626

Alternate gene names: 30064688

Gene position: 3505525-3506880 (Clockwise)

Preceding gene: 30064680

Following gene: 30064689

Centisome position: 76.22

GC content: 52.29

Gene sequence:

>1356_bases
ATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCA
TCGCGAGGCGCTGAAAACGGCGCATATTGCCCTGATGGACATTGACCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGC
GTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAT
TTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCT
GGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTT
GCGAGAACATGACGGAAGTCTGCCCCGATGCCACCATGCTCAACTATGTTAACCCAATGGCGATGAATACCTGGGCGATG
TATGCCCGCTATCCGTATATCAAACAGGTCGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGCTGGCGCGTGACCT
CAATATCGACCCGGCTACGCTGCGTTACCGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAA
CCGCCGACGGCAGTTACGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCAAATATT
CATGGTAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTACTTCGTCACGGAATCATCAGA
ACATTTTGCCGAATATACGCCGTGGTTTATTAAGCTAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATG
AGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAACGCCTCCCGGATTGAT
ATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGT
CCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTC
AGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACC
GAAGCTATTCTTACGGAAAATCGCGACCGTGTTTACCACGCCGCGATGATGGACCCGCATACTGCCGCCGTGCTGGGCAT
TGACGAAATATATGCTCTTGTTGACGACCTGATTGCCGCCCACGGCGACTGGCTGCCAAGCTGGTTGCACCGTTAA

Upstream 100 bases:

>100_bases
GCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTC
AAGCAAGCCAGGAGATCTGC

Downstream 100 bases:

>100_bases
AACGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTT
GGTAACAGCGACCCGATACC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALEDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLHR

Sequences:

>Translated_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALEDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLHR
>Mature_451_residues
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALEDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLHR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=98.8913525498891, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=25.4347826086956, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50715; Mature: 50715

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAMYARYPYIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCHHHHCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPSWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAMYARYPYIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHH
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKLGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCHHHHCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPSWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]