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The map label for this gene is nrfB

Identifier: 30064660

GI number: 30064660

Start: 3472020

End: 3472586

Strand: Direct

Name: nrfB

Synonym: S3598

Alternate gene names: 30064660

Gene position: 3472020-3472586 (Clockwise)

Preceding gene: 30064659

Following gene: 30064661

Centisome position: 75.49

GC content: 53.62

Gene sequence:

>567_bases
ATGAGCGTATTACGTTCGTTATTAACTGCTGGGGTGCTGGCGTCGGGCCTGTTGTGGAGCCTGAACGGAATTACCGCTAC
CCCTGCGGTGCAGGCATCTGGTGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATA
AACCAGATACCGAAGGTATGCATGGCAAACATGCTTCCGTTATCAACCCGAATAACAAATTGCCGGTCACCTGCACCAAC
TGTCACGGCCAAGCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTATAAGGTCGG
AGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAA
CCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATT
AAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCA
GCCATGA

Upstream 100 bases:

>100_bases
CGGTGATCCCGCAGTGGGAAGAGCAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCG
GAAAACACAATGGAGTGAAT

Downstream 100 bases:

>100_bases
CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAA
ACGTTGAATATCAATGGGGT

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQASPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP

Sequences:

>Translated_188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQASPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP
>Mature_187_residues
SVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNC
HGQASPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIK
ICVDCHSDQRTNPNFNPASVPLLKEQP

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=188, Percent_Identity=98.4042553191489, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20659; Mature: 20527

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRNPDAACLDCHKPDTEGM
CHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC
>Mature Secondary Structure 
SVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRNPDAACLDCHKPDTEGM
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]