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The map label for this gene is yfhA

Identifier: 30063941

GI number: 30063941

Start: 2665927

End: 2667261

Strand: Reverse

Name: yfhA

Synonym: S2773

Alternate gene names: 30063941

Gene position: 2667261-2665927 (Counterclockwise)

Preceding gene: 30063942

Following gene: 30063940

Centisome position: 57.99

GC content: 54.31

Gene sequence:

>1335_bases
ATGAGCCATAAACCTGCGCATTTATTATTGGTCGATGACGATCCGGGATTGCTGAAACTGCTTGGCCTGCGCCTGACCAG
CGAAGGCTACAGTGTGGTCACGGCGGAAAGTGGCGCTGAAGGATTACGGGTACTGAATCGCGAAAAAGTAGATTTAGTTA
TCAGCGACCTGCGGATGGATGAAATGGACGGTATGCAGCTATTTGCTGAAATCCAGAAAGTGCAGCCGGGAATGCCAGTA
ATTATTCTTACTGCGCATGGTTCTATCCCCGATGCCGTTGCTGCAACACAGCAGGGCGTTTTTAGTTTCCTCACCAAGCC
TGTCGATAAAGATGCGCTATATCAGGCAATTGACGATGCGCTGGAGCAATCCGCGCCAGCCACCGATGAACGCTGGCGCG
AGGCAATTGTCACCCGCAGCCCGCTGATGCTGCGTTTGCTGGAACAGGCGCGGCTGGTGGCGCAATCAGACGTCAGCGTT
TTGATTAACGGTCAGAGCGGCACCGGGAAAGAGATTTTCGCCCAGGCTATCCACAACGCCAGCCCGCGCAACAGCAAACC
ATTTATTGCTATTAACTGTGGCGCATTGCCCGAGCAATTGCTGGAGTCGGAGCTGTTTGGTCATGCGCGTGGCGCGTTTA
CTGGCGCTGTCAGCAATCGCGAAGGTTTATTCCAGGCGGCGGAAGGCGGAACGCTATTTCTCGATGAAATTGGCGATATG
CCTGCGCCGTTACAGGTCAAACTGCTGCGCGTGTTGCAGGAGCGTAAAGTGCGCCCGCTGGGCAGTAACCGCGATATTGA
TATCAATGTGCGGATTATTTCTGCCACTCACCGTGATCTGTCAAAAGCGATGGCGCGCGGGGAATTCCGCGAAGATCTCT
ATTACCGCCTCAACGTTGTCAGCCTGAAAATTCCGGCACTGGCGGAGCGCACAGAAGACATTCCGCTACTGGCAAATCAC
CTGTTGCGCCAGGCGGCAGAGCGACATAAACCGTTTGTCCGCGCGTTCTCTACCGATGCGATGAAACGCCTGATGACCGC
GAGCTGGCCGGGTAATGTGCGCCAGTTGGTCAACGTGATTGAACAGTGCGTGGCGCTGACCTCATCTCCGGTGATTAGTG
ATGCGCTGGTGGAGCAGGCGCTGGAGGGTGAAAATACGGCGCTGCCAACCTTTGTTGAGGCACGTAATCATTTTGAACTC
AACTACTTGCGTAAGCTGCTGCAAATCACCAAAGGCAACGTCACCCACGCGGCGAGAATGGCGGGGCGCAACCGGACAGA
ATTTTATAAACTGCTTTCCCGACACGAGCTGGATGCAAACGATTTCAAGGAATGA

Upstream 100 bases:

>100_bases
GAACGCCAGCTCTCGACCCGCAAACCGGCTGGAAATTTCTCGCCGGATACGCCGCACGAGAGTGAAAAGCCCGCGCCATC
CACCCATGAGGTCACTCCTG

Downstream 100 bases:

>100_bases
ATTGGCGTTATGTGTTACGTTTAGCAGATCAAAAGACAGGCAACCTTTTCAAGGAATAGCATGAAAAAGATTGATGCGAT
TATAAAACCCTTCAAGCTGG

Product: putative 2-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNHFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNHFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_443_residues
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVI
ILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVL
INGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDMP
APLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHL
LRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNHFELN
YLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=99.5495495495496, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=44.3946188340807, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.2197802197802, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=38.1465517241379, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=55.1724137931034, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1789087, Length=307, Percent_Identity=46.5798045602606, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=48.4126984126984, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=39.873417721519, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1786524, Length=381, Percent_Identity=38.0577427821522, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=37.5776397515528, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=42.6778242677824, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=36, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=33.1210191082803, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49148; Mature: 49017

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMD
CCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHCHHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNHFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMD
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHCHHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNHFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]