Click here to switch to the map view.

The map label for this gene is yfgB

Identifier: 30063909

GI number: 30063909

Start: 2626171

End: 2627325

Strand: Reverse

Name: yfgB

Synonym: S2735

Alternate gene names: 30063909

Gene position: 2627325-2626171 (Counterclockwise)

Preceding gene: 30063910

Following gene: 30063908

Centisome position: 57.12

GC content: 52.73

Gene sequence:

>1155_bases
ATGTCTGAACAATTAGTCACACCTGAAAACGTCACCACGAAAGATGGAAAAATCAACCTGCTGGATCTCAACCGTCAGCA
GATGCGGGAGTTTTTTAAAGATTTAGGTGAAAAAACCTTCCGCGCCGATCAGGTGATGAAGTGGATGTATCACTATTGCT
GCGACAACTTTGATGAGATGACCGACATCAACAAAGTGTTGCGCGGCAAACTGAAAGAGGTGGCGGAAATCCGCGCACCG
GAAGTGGTTGAAGAACAGCGTTCATCCGACGGCACCATTAAATGGGCGATCGCCGTTGGCGATCAGCGCGTCGAAACGGT
GTATATCCCGGAAGACGACCGTGCCACGCTCTGCGTCTCTTCGCAGGTGGGGTGTGCGCTGGAGTGTAAATTCTGTTCCA
CCGCCCAGCAGGGCTTTAACCGCAACCTGCGGGTATCGGAAATTATTGGCCAGGTGTGGCGTGCGGCGAAAATCGTCGGC
GCAGCAAAAGTCACCGGTCAGCGCCCAATCACTAACGTCGTGATGATGGGCATGGGCGAGCCGCTGCTCAACCTGAACAA
CGTCGTTCCGGCGATGGAAATCATGCTTGATGATTTCGGTTTTGGCCTGTCTAAACGTCGCGTGACGCTTTCCACTTCCG
GTGTAGTTCCGGCGCTGGATAAACTGGGCGATATGATCGACGTTGCACTGGCAATTTCCCTGCACGCGCCGAACGACGAA
ATTCGTGACGAAATTGTGCCGATCAACAAAAAGTACAATATCGAAACGTTCCTTGCTGCGGTGCGCCGTTATCTGGAGAA
ATCCAACGCCAATCAGGGCCGAGTCACTATTGAATACGTGATGCTTGACCACGTTAACGACGGCACTGAACACGCGCACC
AACTGGCGGAACTGCTGAAAGATACGCCGTGTAAGATCAACCTGATCCCGTGGAACCCGTTCCCGGACGCGCCGTATGGA
CGCAGTTCGAACAGCCGTATCGACCGTTTCTCAAAAGTGCTGATGAGCTACGGTTTCACCACTATTGTGCGTAAAACGCG
TGGTGATGATATCGATGCTGCCTGTGGTCAGTTGGCGGGCGATGTTATCGACCGTACGAAACGTACTCTGCGTAAACGGA
TGCAGGGTGAAGCCATCGATATTAAAGCGGTCTGA

Upstream 100 bases:

>100_bases
GTACAATGCAGCGCCCCCGGACGAGCAGCCGCTCACCGGGGCGTTTCTTTTTTCAACCCTCCAGGGGCCATAACGTGTAA
TAACGAGGCCGGAATAGCAT

Downstream 100 bases:

>100_bases
TAGCCATTACGCCACGGTTACTTTATGTACCGTGGCGTAATCTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCA
ATATTAACGGTGCGTTTTTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV

Sequences:

>Translated_384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV
>Mature_383_residues
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPE
VVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGA
AKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDEI
RDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGR
SSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=99.4791666666667, Blast_Score=793, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43148; Mature: 43017

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDIKAV
HHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDIKAV
HHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA