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The map label for this gene is ispG

Identifier: 30063907

GI number: 30063907

Start: 2623719

End: 2624846

Strand: Reverse

Name: ispG

Synonym: S2733

Alternate gene names: 30063907

Gene position: 2624846-2623719 (Counterclockwise)

Preceding gene: 30063908

Following gene: 30063906

Centisome position: 57.07

GC content: 53.19

Gene sequence:

>1128_bases
ATGCATAACCAGGCTCCAATTCAACGTAGAAAATCAACACGTATTTACGTTGGGAATGTGCCGATTGGCGATGGTGCTCC
CATCGCCGTACAGTCCATGACCAATACGCGTACGACAGATGTCGAAGCAACGGTCAATCAAATCAAGGCGCTGGAACGCG
TTGGCGCTGATATCGTCCGTGTCTCCGTACCGACGATGGACGCGGCAGAAGCGTTCAAACTCATCAAACAGCGGGTTAAC
GTGCCGCTGGTGGCTGACATCCACTTCGACTATCGCATTGCGCTGAAAGTAGCGGAATACGGCGTCGATTGTCTGCGTAT
TAACCCTGGTAATATCGGTAATGAAGAGCGTATTCGCATGGTGGTTGACTGTGCGCGCGATAAAAACATTCCGATCCGTA
TTGGCGTTAACGCCGGATCGCTGGAAAAAGATCTGCAAGAAAAGTATGGCGAACCGACGCCGCAGGCGTTGCTGGAATCC
GCCATGCGTCATGTTGATCATCTCGATCGCCTGAACTTCGATCAGTTCAAAGTCAGCGTGAAAGCGTCTGACGTCTTCCT
CGCTGTTGAGTCTTATCGTTTGCTGGCAAAACAGATCGATCAGCCGCTGCATCTGGGGATCACCGAAGCCGGTGGTGCAC
GCAGCGGGGCAGTAAAATCCGCCATTGGTTTAGGTCTGCTGCTGTCGGAAGGCATCGGCGACACGCTGCGTGTATCGCTG
GCGGCCGATCCGGTCGAAGAGATCAAAGTCGGTTTCGATATTTTGAAATCGCTGCGTATCCGTTCGCGAGGGATTAACTT
CATCGCCTGCCCGACCTGTTCGCGTCAGGAATTTGATGTTATCGGTACGGTTAACGCGCTGGAGCAACGCCTGGAAGATA
TCATCACTCCGATGGACGTTTCGATTATCGGCTGCGTGGTGAATGGCCCAGGTGAGGCGCTGGTTTCTACACTCGGCGTC
ACCGGCGGCAACAAGAAAAGCGGCCTCTATGAAGATGGCGTGCGCAAAGACCGTCTGGACAACAACGATATGATCGACCA
GCTGGAAGCACGCATTCGTGCGAAAGCCAGTCAGCTGGACGAAGCGCGTCGAATTGACGTTCAGCAGGTTGGAAAAATAA
TAACGTGA

Upstream 100 bases:

>100_bases
ATCTGAGTCGTTTTATCAGAACTAACCAGGTTGCGCGTCTGACCCTCAATGCCGAACAATCACCGGCGCAGTAACAGACG
GGTAACGCGGGAGATTTTTC

Downstream 100 bases:

>100_bases
TGGGAAGCGCCTCGCTTCCCGTGTATGATTGAACCCGCATGGCTCCCGAAACATTGAGGGAAGCGTTGAGGGTTCATTTT
TATATTCAGAAAGAGAATAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT

Sequences:

>Translated_375_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT
>Mature_375_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=99.4623655913979, Blast_Score=747, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_SHIF8 (Q0T204)

Other databases:

- EMBL:   CP000266
- RefSeq:   YP_689966.1
- ProteinModelPortal:   Q0T204
- STRING:   Q0T204
- EnsemblBacteria:   EBESCT00000097490
- GeneID:   4207521
- GenomeReviews:   CP000266_GR
- KEGG:   sfv:SFV_2562
- eggNOG:   COG0821
- GeneTree:   EBGT00050000011293
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   SFLE373384:SFV_2562-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40968; Mature: 40968

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVR
CCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRM
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHEE
VVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
EEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
EECEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLD
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EARRIDVQQVGKIIT
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVR
CCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRM
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHEE
VVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
EEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
EECEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLD
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EARRIDVQQVGKIIT
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA