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The map label for this gene is yfbT

Identifier: 30063719

GI number: 30063719

Start: 2399008

End: 2399658

Strand: Reverse

Name: yfbT

Synonym: S2504

Alternate gene names: 30063719

Gene position: 2399658-2399008 (Counterclockwise)

Preceding gene: 30063720

Following gene: 30063718

Centisome position: 52.17

GC content: 56.07

Gene sequence:

>651_bases
GTGCGGTGCAAAGGTTTTCTGTTTGATCTTGATGGAACGCTGGTGGATTCCCTGCCTGCGGTAGAACGGGCGTGGAGCAA
CTGGGCCAGACGTCATGGGTTAGCGCCGGAAGAGGTGCTGGCTTTCATTCACGGTAAACAGGCGATCACCTCTTTGCGCC
ATTTTATGGCGGGCAAATCCGAGGCTGATATTGCCGCCGAGTTTACGCGTCTGGAGCAAATCGAGGCCACGGAAACCGAA
GGTATTACCGCGCTTCCTGGGGCAATCGCCTTACTCAATCATTTGAATAAAGCAGGTATTCCGTGGGCCATTGTGACTTC
TGGCTCCATGCCGGTAGCGCGAGCGCGCCATAAAATAGCGGGGCTTCCCGCACCAGAGGTGTTTGTAACCGCTGAGCGAG
TGAAGCGCGGAAAACCAGAACCTGATGCGTATCTGTTAGGTGCGCAGCTGCTGGGGCTTGCGCCGCAGGAGTGTGTGGTG
GTGGAAGATGCTCCCGCTGGCGTGCTTTCTGGCCTGGCGGCGGGTTGCCATGTCATTGCGGTTAACGCTCCGGCAGATAC
CCCGCGCCTGAATGAGGTCGATTTGGTCCTCCACAGTCTGGAGCAAATTACTGTGACCAAACAGCCAAATGGCGATGTTA
TTATTCAGTGA

Upstream 100 bases:

>100_bases
AAATATCAGCGCATGCTTAATGTGTGGCATGCCTGCCCGCGTCAGTACCATTTGAGCGCCAACGAAATTAATCAAATTAT
CAACGCCTGAGGAGGCCCGC

Downstream 100 bases:

>100_bases
AACACCTGATCATGATTTAGCCCCGTCTCGTCGGGGCTTTTTTATGGCAGAATCAAGTCATCCCCCTCAATTAACAAGGA
TAAGTTGTGAACGGTGAATT

Product: putative phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Sequences:

>Translated_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
>Mature_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETE
GITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Homo sapiens, GI197382691, Length=208, Percent_Identity=31.25, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI207113149, Length=233, Percent_Identity=27.8969957081545, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87082080, Length=216, Percent_Identity=99.0740740740741, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1790151, Length=202, Percent_Identity=30.6930693069307, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789046, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321834, Length=229, Percent_Identity=32.3144104803493, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321833, Length=229, Percent_Identity=31.8777292576419, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322136, Length=219, Percent_Identity=31.9634703196347, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320905, Length=221, Percent_Identity=33.0316742081448, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129151, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17137324, Length=198, Percent_Identity=29.2929292929293, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23026; Mature: 23026

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC
>Mature Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]