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The map label for this gene is rusA [H]

Identifier: 30063426

GI number: 30063426

Start: 2037422

End: 2037787

Strand: Reverse

Name: rusA [H]

Synonym: S2140

Alternate gene names: 30063426

Gene position: 2037787-2037422 (Counterclockwise)

Preceding gene: 30063427

Following gene: 30063425

Centisome position: 44.31

GC content: 52.19

Gene sequence:

>366_bases
ATGACCGAACGCATAGAATTTGTTTTGCCTTACCCGCCAACGGTGAACACCTACTGGCGACGTCGTGGCAGCACATATTT
TGTATCAAAAGCCGGTGAGCGTTATCGCCGTGATGTGGCGCTAATTGTTCGCCAGCAGCGACTGAAATTAAACCTGTCCG
GAAGGCTGGCGATAAAGATTATTGCAGAGCCACCGGATAAGCGCCGTCGTGACCTGGACAATATCCTGAAGGCACCGCTG
GATGCGCTGACGCATGCTGGACTACTTATAGACGACGAGCAGTTTGATGAAATCAATATTGTGCGCGGTCAGCTCGTTCC
TGGTGGGCGGCTGGGGATAAAAATCACAGAACTGGGGTGCGCATGA

Upstream 100 bases:

>100_bases
GGTAAAAGATTTTGAAGAAAAACACGGTAGTCAGTTGTTGTTGCTGATTCGTTTTTTAATGCACGCGAGAAATTCGGGTG
TTCTGAAGTGGAAAGCATAA

Downstream 100 bases:

>100_bases
ATAACCAGTATTTACAGTTTGTGCGTGAGCAGCTCATTATCGCCACCGCTGATTTGAGTGGGGCAACAAAAGGTCAGCTT
GAAGCCTGGCAAGAGAATGC

Product: putative crossover junction endodeoxyribonuclease

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPL
DALTHAGLLIDDEQFDEINIVRGQLVPGGRLGIKITELGCA

Sequences:

>Translated_121_residues
MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPL
DALTHAGLLIDDEQFDEINIVRGQLVPGGRLGIKITELGCA
>Mature_120_residues
TERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLD
ALTHAGLLIDDEQFDEINIVRGQLVPGGRLGIKITELGCA

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=114, Percent_Identity=42.1052631578947, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 13841; Mature: 13710

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKI
CCCCEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEE
IAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQLVPGGRLGIKITELGC
EECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEEECCCCCCEEEEEECCC
A
C
>Mature Secondary Structure 
TERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSGRLAIKI
CCCEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEE
IAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQLVPGGRLGIKITELGC
EECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEEECCCCCCEEEEEECCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA