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The map label for this gene is insF1 [C]

Identifier: 30063325

GI number: 30063325

Start: 1932840

End: 1933274

Strand: Reverse

Name: insF1 [C]

Synonym: S2009

Alternate gene names: 30063325

Gene position: 1933274-1932840 (Counterclockwise)

Preceding gene: 30063326

Following gene: 30063323

Centisome position: 42.03

GC content: 52.18

Gene sequence:

>435_bases
ATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGTGCCATGGAAACGACATTCGTGCTGGATGCACTGGA
GCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACA
CACAGCGGCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGC
ATCAATGGTCTTTACAAAGCGGAGGTAATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTAAC
GTGGGTGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCCATACTCCTCCGGCAGAAGCAGAAAAAGCTTATT
ATGCTTCCATCGGAAACGATGATCTGGCAGCCTGA

Upstream 100 bases:

>100_bases
GCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAG
CACATGGCGGGGCTTCGTCT

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCAGGGCGGTTAATTCCACCGCTAAAGGTACAACGGAATGA
AGTGATTGTGAATTATCGCT

Product: IS629 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MWRSSLMCLPDTSWGAMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_144_residues
MWRSSLMCLPDTSWGAMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
>Mature_144_residues
MWRSSLMCLPDTSWGAMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=91, Percent_Identity=56.043956043956, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788405, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787263, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786753, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786570, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786490, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16126; Mature: 16126

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRSSLMCLPDTSWGAMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHC
GLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLER
CCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEHHHCCHHHHHH
LGHTPPAEAEKAYYASIGNDDLAA
CCCCCCCCCCHHEEEECCCCCCCC
>Mature Secondary Structure
MWRSSLMCLPDTSWGAMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHC
GLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLER
CCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEEHHHCCHHHHHH
LGHTPPAEAEKAYYASIGNDDLAA
CCCCCCCCCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]