Click here to switch to the map view.

The map label for this gene is ntpA

Identifier: 30063275

GI number: 30063275

Start: 1881248

End: 1881730

Strand: Reverse

Name: ntpA

Synonym: S1941

Alternate gene names: 30063275

Gene position: 1881730-1881248 (Counterclockwise)

Preceding gene: 30063276

Following gene: 30063274

Centisome position: 40.91

GC content: 52.38

Gene sequence:

>483_bases
ATGAGCGAAGGCAGTCAACGAAGAGGCAGCGTGAAGGATAAAGTGTATAAGCGTCCCGTTTCGATCTTAGTGGTCATCTA
TGCACAAGATACGAAACGGGTGCTGATGTTGCAGCGGCGTGACGATCCCGATTTCTGGCAGTCGGTAACCGGCAGCGTGG
AAGAGGGTGAAACCGCGCCGCAAGCTGCCATGCGCGAAGTAAAGGAAGAGGTTACCATTGATGTTGTCGCTGAACAACTG
ACCTTAATTGACTGTCAGCGCACGGTAGAGTTTGAAATTTTTTCACATTTACGTCATCGCTATGCGCCGGGCGTGACGCG
TAATACGGAATCATGGTTCTGTCTTGCGCTTCCGCACGAGCGGCAGATCGTTTTCACTGAACATCTGGCTTACAAGTGGC
TTGATGCGCCTGCTGCGGCGGCGCTCACTAAGTCCTGGAGCAACCGGCAGGCGATTGAACAGTTTGTAATTAACGCTGCC
TGA

Upstream 100 bases:

>100_bases
TGAGAATAACTGATATGACTCAAATACACGAAATCATTCGCGTTGCATCAAGGCGGCAACTGAGTGAACTCCCATGAGCA
TAGATAACTATGTGAATGGG

Downstream 100 bases:

>100_bases
ACAGGCAGGGCTATCTGGAGATATTTTTATGGCAGGTCATAGTAAATGGGCCAACACCAGACATCGTAAAGCTGCGCAGG
ATGCTAAGCGCGGTAAAATC

Product: dATP pyrophosphohydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQL
TLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Sequences:

>Translated_160_residues
MSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQL
TLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
>Mature_159_residues
SEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLT
LIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=150, Percent_Identity=99.3333333333333, Blast_Score=310, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 18350; Mature: 18219

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAP
CCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCH
QAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
RQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
CEEEEHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAP
CCCCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCH
QAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
RQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
CEEEEHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]