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The map label for this gene is msbB

Identifier: 30063266

GI number: 30063266

Start: 1872291

End: 1873262

Strand: Reverse

Name: msbB

Synonym: S1931

Alternate gene names: 30063266

Gene position: 1873262-1872291 (Counterclockwise)

Preceding gene: 30063267

Following gene: 30063263

Centisome position: 40.73

GC content: 51.34

Gene sequence:

>972_bases
ATGGAAACGAAAAAAAATAATAGCGAATACATTCCTGAGTTTGATAAATCCTTTCGCCACCCGCGCTACTGGGGAGCATG
GCTGGGTGTAGCAGCGATGGCGGGTATCGCTTTAACGCCGCCAAAGTTCCGTGATCCCATTCTGGCACGGCTGGGACGTT
TTGCCGGACGACTGGGAAAAAGCTCACGCCGTCGTGCGTTAATCAATCTGTCGCTCTGCTTTCCAGAACGTAGTGAAGCT
GAACGCGAAGCGATTGTTGATGAGATGTTTGCCACCGCGCCGCAAGCGATGGCAATGATGGCTGAGTTGGCAATACGCGG
GCCGGAGAAAATTCAGCCGCGCGTTGGCTGGCAAGGGCTGGAGATCATCGAAGAGATGCGGCGTAATAACGAGAAAGTTA
TCTTTCTGGTGCCGCACGGTTGGGCCGTCGATATTCCTGCCATGCTGATGGCCTCGCAAGGGCAGAAAATGGCAGCGATG
TTCCATAATCAGGGCAACCCGGTTTTTGATTATGTCTGGAACACGGTGCGTCGTCGCTTTGGCGGTCGTCTGCATGCGAG
AAATGACGGTATTAAACCATTCATCCAGTCGGTACGTCAGGGGTACTGGGGATATTATTTACCCGATCAGGATCATGGCC
CAGAGCACAGCGAATTTGTGGATTTCTTTGCCACCTATAAAGCGACGTTGCCCGCGATTGGTCGTTTGATGAAAGTATGC
CGTGCGCGCGTTGTACCGCTGTTTCCGATTTATGATGGCAAGACGCATCGTCTGACGATTCAGGTGCGCCCACCGATGGA
TGATCTGTTAGAGGCGGATGATCATACGATTGAGCGGCGGATGAATGAAGAAGTCGAGATTTTTGTTGGTCCGCGACCAG
AACAATACACCTGGATACTAAAATTGCTGAAAACTCGCAAACCGGGCGAAATCCAGCCGTATAAGCGCAAAGATCTTTAT
CCCATCAAATAA

Upstream 100 bases:

>100_bases
GGTACGCAGTCAGTACCGGCTTTTTTTATTTGGTGCGGGGCAAGTTGTGCCGCTACACTATCACCAGATTGATTTTTGCC
TTATCCGAAACTGGAAAAGC

Downstream 100 bases:

>100_bases
AAAAGCCTCTCGCGAGGAGAGGCCTTCGCCTGATGATAAGTTCAAGTTTGCTTCAGAATATTCGAAATCTGTTGAACTAT
CATTGAACTATAGGCCGGAT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Sequences:

>Translated_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK
>Mature_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family

Homologues:

Organism=Escherichia coli, GI1788161, Length=323, Percent_Identity=99.3808049535604, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=312, Percent_Identity=30.4487179487179, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=25.4071661237785, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSBB1_SHIFL (P59198)

Other databases:

- EMBL:   AE005674
- EMBL:   AE014073
- RefSeq:   NP_707715.1
- RefSeq:   NP_837437.1
- EnsemblBacteria:   EBESCT00000085378
- EnsemblBacteria:   EBESCT00000092437
- GeneID:   1027792
- GeneID:   1078262
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF1865
- KEGG:   sfx:S1931
- GeneTree:   EBGT00050000010320
- HOGENOM:   HBG730280
- OMA:   FGTYKAT
- ProtClustDB:   PRK08943
- BioCyc:   SFLE198214:AAN43422.1-MONOMER
- InterPro:   IPR004960
- InterPro:   IPR011921
- TIGRFAMs:   TIGR02208

Pfam domain/function: PF03279 Lip_A_acyltrans

EC number: 2.3.1.-

Molecular weight: Translated: 37411; Mature: 37411

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12bb0bfc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVGWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIERRMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152