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The map label for this gene is mcbR [H]

Identifier: 30063237

GI number: 30063237

Start: 1841282

End: 1842004

Strand: Reverse

Name: mcbR [H]

Synonym: S1897

Alternate gene names: 30063237

Gene position: 1842004-1841282 (Counterclockwise)

Preceding gene: 30063238

Following gene: 30063229

Centisome position: 40.05

GC content: 43.71

Gene sequence:

>723_bases
GTGAACTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAA
ACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCC
TGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTG
AATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTACCGGAAGTGAGGCAAAGTCAGTTAGACGAAATCAATCGGAT
CCGCTACGAACTGGAATTAATGGCAGTTGCCCTGGCTGTTGAAAACCTCTCCTCGCAAGACATAGCAGAACTTCAGGAAT
TGCTCGGGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTGAACAGGCTATTTCGCTTAGCG
ATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGATCAGGATGGGACCTGCTTTACATTA
TCTTTATGAAGCGATTAACCCAGCGGAATTACGGGAGCGCATAGAAAACTATCATCTATTACTCGCTGCGTTAAAAGCAA
AAGACAAAGAGGGATGCAAACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGT
TAA

Upstream 100 bases:

>100_bases
AAAAGCTTATTTTCAGCTTTAATTAACTAACCACGTCATTGTTCTGCTGCTAATAACAACAAAGGTGGGTGGTTTATGAT
TATTCACTTTAATACACTAG

Downstream 100 bases:

>100_bases
ATTAAATGCCCGGCAAATACCGGGCATCAAGTAATTACTCAAATCGCCACGCAATATTCATGCCGACACCATAATTTCGT
TCATCGATAGGAATTAAAAC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNSFCHAGRVGQKTREGIKMPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSV
NALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLA
IYHRSNMPILCEMIEQLWIRMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQYNR

Sequences:

>Translated_240_residues
MNSFCHAGRVGQKTREGIKMPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSV
NALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLA
IYHRSNMPILCEMIEQLWIRMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQYNR
>Mature_240_residues
MNSFCHAGRVGQKTREGIKMPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSV
NALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLA
IYHRSNMPILCEMIEQLWIRMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQYNR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=221, Percent_Identity=95.0226244343891, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27206; Mature: 27206

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFCHAGRVGQKTREGIKMPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLA
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHH
EQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAV
HHHCCCCCHHHHHHHHHHHCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWIR
HCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQYNR
HCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSFCHAGRVGQKTREGIKMPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLA
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHH
EQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAV
HHHCCCCCHHHHHHHHHHHCCHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWIR
HCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQYNR
HCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]