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The map label for this gene is uidA

Identifier: 30063131

GI number: 30063131

Start: 1709940

End: 1711643

Strand: Reverse

Name: uidA

Synonym: S1771

Alternate gene names: 30063131

Gene position: 1711643-1709940 (Counterclockwise)

Preceding gene: 30063132

Following gene: 161486483

Centisome position: 37.21

GC content: 52.11

Gene sequence:

>1704_bases
ATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAA
CTGTGGAATTGATCAGCGTTGGTGCGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCAGTGCCAGGCAGTTTTAACGATC
AGTTCGCCGATGCAGATATTCGTAATTATGTGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCA
GGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCA
TCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTG
TGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAAACGGCAAGAAAAAGCAGTCTTAC
TTCCATGATTTCTTTAACTATGCCGGGATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATAT
CACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATATCAGCG
TTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACCAGCGGGACTTTGCAAGTGGTGAATCCGCAC
CTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGTGCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCT
GCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTG
GTCGTCATGAAGATGCGGATTTGCGAGGCAAAGGATTCGATAACGTGCTGATGGTGCACGATCACGCATTAATGGACTGG
ATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGT
GGTGATTGATGAAACTGCAGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAAC
TGTACAGCGAAGAGGCTGTCAACGGGGAAACTCAGCAGGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAA
AACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCC
ACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCG
ATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGTTGGTATGTCCAAAGCGGCGATTTGGAAACG
GCAGAGAAGGTACTGGAAAAAGAACTGCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGT
GGATACGTTAGCCGGCCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC
ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATA
TTGCGCGTTGGCGGTAACAATTGA

Upstream 100 bases:

>100_bases
CTTCCATGGGTTTCTCACAGATAACTGTGTGCAACACAGAATTGGTTAACTAATCAGATTAAAGGTTGACCAGTATTTTT
ATCTTAATGAGGAGTCCCTT

Downstream 100 bases:

>100_bases
CCTACATGGGCCTCGGGCTTTGCTACAGCCTGGTGAATATTCCTTATGGTTCACTTGCTACCGCGATGACCCAACAACCA
CAATCTCGCGCCCGTCTGGG

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNN

Sequences:

>Translated_567_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNN
>Mature_567_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNN

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=600, Percent_Identity=44.3333333333333, Blast_Score=471, Evalue=1e-133,
Organism=Escherichia coli, GI1787903, Length=567, Percent_Identity=99.294532627866, Blast_Score=1179, Evalue=0.0,
Organism=Escherichia coli, GI1786539, Length=481, Percent_Identity=26.1954261954262, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994920, Length=390, Percent_Identity=25.3846153846154, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=595, Percent_Identity=35.7983193277311, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI62471735, Length=599, Percent_Identity=42.7378964941569, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19922582, Length=599, Percent_Identity=42.7378964941569, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24655438, Length=599, Percent_Identity=42.7378964941569, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651745, Length=608, Percent_Identity=39.3092105263158, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 64275; Mature: 64275

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHHHCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNN
HHHHHHHHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHHHCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNN
HHHHHHHHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]