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The map label for this gene is bioD

Identifier: 30063108

GI number: 30063108

Start: 1680782

End: 1681477

Strand: Reverse

Name: bioD

Synonym: S1746

Alternate gene names: 30063108

Gene position: 1681477-1680782 (Counterclockwise)

Preceding gene: 30063109

Following gene: 30063103

Centisome position: 36.56

GC content: 52.01

Gene sequence:

>696_bases
ATGCTGAAGCGTTTCTTTATTACCGGTACAGACACTTCTGTAGGGAAAACGGTGGTTTCCCGCGCATTGCTACAAGCGTT
AGCCTCCCAGGGAAAAAGGGTTGCGGGATATAAACCCGTAGCGAAGGGGAGCAAAGAGACACCCGAAGGGCTGCGTAATA
AAGATGCCTTGGTGTTGCAGAGTGTTTCAACCATCGAACTGCCTTATGAAGCAGTTAATCCTATCGCGTTAAGCGAAGAA
GAAAGTAGCGTGGCGCACAGTTGCCCAATCAATTACACCCTCATTTCAAACGGCCTGGCAAACCTGACCGATAAAGTCGA
TCATGTCGTGGTAGAAGGGACTGGCGGCTGGCGCAGTCTGATGAATGATTTGCGTCCACTCTCTGAATGGGTAGTGCAGG
AACAACTGCCGGTGTTGATGGTTGTCGGTATTCAGGAAGGTTGCATTAACCATGCACTGCTAACAGCTCAGGCGATCGCC
AACGACGGGCTGCCGCTCATTGGCTGGGTGGCTAACCGAATCAACCCAGGACTGGCGCATTATGCGGAAATCATTGATGT
GCTGGGTAAAAAACTTCCGGCACCGCTCATTGGTGAACTGCCTTATCTGCCGCGCGCCGAACAGCGTGAACTGGGGCAAT
ACATCCGCTTAGCTATGCTGCGCAGTGTGCTGGCGGTAGATAGAGTCACGGTGTAA

Upstream 100 bases:

>100_bases
TTTGCGCTATCTCAATTTGGGCCAGGAAAGCATAACTTAGACTTTCAAGGTTAATTATTTTCCTAGTTTATATTTGTGAA
GCATAACGGTGGAGTTAGTG

Downstream 100 bases:

>100_bases
CGTCCGCGAAATTACCGACGCAATTACGCAGGCAATCAATAAACCGGGGAGTAGCTGATACTCCCCGGTCATTTCACATA
TCATCAACGTCGACATAATC

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV

Sequences:

>Translated_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
>Mature_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=231, Percent_Identity=99.1341991341991, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=51.5555555555556, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25022; Mature: 25022

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGSKETPEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHH
SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHHCCCCEEHHHCCHHHHHHHHCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGSKETPEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHH
SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHHCCCCEEHHHCCHHHHHHHHCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]