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The map label for this gene is rspA

Identifier: 30063091

GI number: 30063091

Start: 1660824

End: 1662038

Strand: Reverse

Name: rspA

Synonym: S1718

Alternate gene names: 30063091

Gene position: 1662038-1660824 (Counterclockwise)

Preceding gene: 30063092

Following gene: 30063090

Centisome position: 36.14

GC content: 50.78

Gene sequence:

>1215_bases
ATGAAGATCGTAAAGGCTGAAGTTTTTGTTACCTGTCCGGGGCGTAATTTCGTCACATTAAAAATCACCACTGAGGACGG
TATTACGGGCCTTGGGGATGCCACCCTCAATGGACGTGAGCTTTCCGTGGCCTCTTATTTGCAGGATCATCTTTGTCCGC
GGCTTATTGGTCGCGATGCGCACCGTATCGAAGATATCTGGCAGTTTTTCTATAAAGGTGCTTACTGGCGTCGCGGTCCG
GTTACGATGTCGGCCATTTCAGCGGTTGATATGGCGCTGTGGGATATTAAAGCCAAAGCTGCCAACATGCCGCTTTACCA
GTTACTCGGCGGCGCGTCTCGTGAAGGGGTGATGGTTTATTGCCATACCACCGGTCACAGTATTGATGAAGCTCTGGATG
ATTATGCCCGTCATCAGGAGCTGGGATTCAAAGCCATCCGCGTGCAGTGCGGAATCCCTGGTATGAAAACCACCTACGGC
ATGTCGAAAGGTAAAGGTCTGGCTTATGAACCCGCAACCAAAGGACAGTGGCCGGAAGAGCAGCTGTGGTCGACGGAGAA
ATACCTCGATTTCATGCCGAAATTGTTTGACGCGGTACGTAACAAGTTTGGTTTTAATGAACATTTGCTGCATGACATGC
ACCATCGCTTAACGCCTATTGAAGCGGCGCGCTTTGGTAAAAGCATTGAAGATTATCGCATGTTCTGGATGGAAGACCCG
ACGCCAGCGGAAAACCAAGAATGCTTCCGTCTCATTCGCCAACATACCGTCACACCCATCGCAGTGGGTGAAGTCTTCAA
CAGCATCTGGGACTGCAAACAACTGATTGAAGAGCAACTCATCGATTATATCCGCACCACGCTGACCCATGCAGGCGGAA
TTACCGGTATGCGCCGGATTGCCGATTTTGCTTCGCTGTATCAGGTACGTACTGGCTCACACGGTCCTTCCGATTTGTCA
CCAGTCTGCATGGCTGCGGCGCTGCACTTTGATCTGTGGGTCCCCAATTTCGGTGTCCAGGAATACATGGGTTATTCCGA
ACAAATGCTCGAAGTCTTCCCGCACAACTGGACTTTCGATAACGGCTATATGCATCCGGGAGACAAACCGGGTCTTGGTA
TCGAATTCGATGAAAAGCTGGCGGCGAAATATCCCTATGAACCTGCTTATCTACCAGTCGCACGTCTGGAAGATGGCACG
CTGTGGAACAGGTAA

Upstream 100 bases:

>100_bases
TTTGTGATCACTCCGGCTTTTTTCGATCTTCATACTTGTATGGCAGTAGCTCAGTTGCGTAGATTTCATGCATCACGACA
AGCGATGCAAGGAATCGAAC

Downstream 100 bases:

>100_bases
GGAGTAAGATAATTGAATCGCCACGGGTTTAACAGACACCTCAGAGTCATTTAAGATGGCTTAAAGAGAGGTGCCCATGA
GCGGTAAGCGTTATCCCGAA

Product: starvation sensing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNR

Sequences:

>Translated_404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNR
>Mature_404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNR

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=403, Percent_Identity=99.7518610421836, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=386, Percent_Identity=31.0880829015544, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45967; Mature: 45967

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPRLIGRDA
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
HRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCEECCCCCCCCCHH
QLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCCCCC
NGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNR
CCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPRLIGRDA
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
HRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCEECCCCCCCCCHH
QLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCCCCC
NGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNR
CCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]