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The map label for this gene is rus

Identifier: 30063047

GI number: 30063047

Start: 1617635

End: 1617991

Strand: Reverse

Name: rus

Synonym: S1665

Alternate gene names: 30063047

Gene position: 1617991-1617635 (Counterclockwise)

Preceding gene: 30063048

Following gene: 30063046

Centisome position: 35.18

GC content: 52.38

Gene sequence:

>357_bases
ATGCTCATAGATTTGGTTTTACCTTACCCGCCGACGGTGAACACTTACTGGCGACGCCGTGGCAGCACATATTTTATCTC
GGAGGAGGGAAAGCGTTATCGCCGGGCTGTGGCGCTTATTGTTCGCCAGCAGCGGCTGAAATTAAGCCTGTCCGGAAGGC
TGGCGATAAAGGTGATTGCAGAGCCACCGGATAAGCGTCGTCGCGACCTGGACAATATCCTGAAAGCACCGCTGGATGCG
CTGACGCATGCGGGAGTGTTAATGGACGATGAGCAGTTTGATGAAATCAATATCGTTCGTGGTCAGCCAGTATCTGGTGG
ACGTCTGGGGGTGAAGATTTACCCCATAATGCATTAA

Upstream 100 bases:

>100_bases
TCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACTGGCGTGC
TTGGGTAAAAGAGGTGACTG

Downstream 100 bases:

>100_bases
GAGCAGGTCAAAAAATGAAACTGGAAGATTTACCGAAATACTACTCCCCAAAATCCCCTGGCCTGACCGATGCATCGGCC
TCAACGTCAAAAGATGCGCT

Product: endodeoxyribonuclease RUS (Holliday junction resolvase)

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMH

Sequences:

>Translated_118_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMH
>Mature_118_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMH

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=109, Percent_Identity=40.3669724770642, Blast_Score=87, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 13538; Mature: 13538

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIA
CEEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMH
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIA
CEEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMH
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA