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The map label for this gene is sapA

Identifier: 30062815

GI number: 30062815

Start: 1349605

End: 1351248

Strand: Reverse

Name: sapA

Synonym: S1381

Alternate gene names: 30062815

Gene position: 1351248-1349605 (Counterclockwise)

Preceding gene: 30062816

Following gene: 30062814

Centisome position: 29.38

GC content: 54.2

Gene sequence:

>1644_bases
ATGCGCCAGGTATTATCGTCTCTTTTGGTGATTGCTGGACTTGTGAGTGGTCAGGCAATCGCCGCGCCTGAATCCCCCCC
GCATGCTGATATCCGCGACAGCGGTTTTGTCTATTGCGTCAGCGGGCAAGTCAACACCTTTAACCCATCCAAAGCGAGCA
GTGGGTTAATTGTCGATACCCTTGCCGCCCAGTTTTATGATCGACTGCTGGATGTCGATCCCTATACCTATCGCCTGATG
CCGGAACTTGCCGAAAGCTGGGAAGTACTCGACAACGGCGCGACCTATCGCTTCCACCTGCGTCGCGATGTTCCGTTTCA
AAAAACCGACTGGTTTACTCCCACTCGTAAAATGAATGCCGACGATGTGGTGTTTACCTTCCAGCGAATTTTTGACCGCA
ACAACCCGTGGCATAACGTCAACGGCAGCAACTTCCCCTACTTCGACAGCCTGCAATTTGCCGATAACGTGAAAAGCGTC
CGCAAACTGGATAATCATACCGTTGAGTTCCGACTCGCCCAGCCGGATGCTTCTTTTTTGTGGCACCTCGCAACCCATTA
TGCTTCGGTCATGTCGGCAGAATATGCCCGGAAGTTAGAGAAAGAAGATCGCCAGGAGCAACTCGACCGTCAACCGGTCG
GCACCGGTCCGTATCAGTTGTCGGAATACCGCGCCGGGCAATTTATTCGCCTACAACGTCATGATGACTTCTGGCGCGGT
AAACCGTTAATGCCGCAGGTAGTGGTGGATTTAGGCTCCGGCGGCACCGGACGTCTGTCGAAACTCCTGACCGGGGAATG
CGACGTTCTGGCCTGGCCTGCTGCCAGCCAGCTATCCATTTTGCGTGACGACCCGCGCTTGCGTTTAACGCTGCGTCCTG
GGATGAACGTCGCCTATCTGGCATTTAACACCGCCAAACCGCCGCTAAATAATCCCGCTGTCCGCCATGCACTGGCACTG
GCGATTAATAACCAGCGCCTGATGCAATCCATCTATTATGGTACGGCTGAAACGGCGGCCTTTATTTTACCGCGCGCCTC
GTGGGCCTATGACAACGAGGCTAAAATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGGCGTTGGGGCTGG
AAAACTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTGATT
CAGGCGGATATGGCGCAGGTTGGCGTAAAAGTGGTGATTGTGCCGGTAGAAGGTCGCTTTCAGGAGGCGCGGTTGATGGA
TATGAGCCATGATCTGACGTTATCCGGTTGGGCGACGGACAGTAACGACCCGGACAGTTTCTTCCGTCCGTTACTGAGCT
GCGCGGCAATTCATTCACAGACCAACCTCGCCCACTGGTGCAATCCGAAATTCGACAGCGTGTTGCGTAAGGCGCTCTCC
TCGCAGCAGCTGGCAGCGCGTATTGAAGCCTATGACGAAGCGCAGAGTATTCTGGCGCAGGAATTACCTATTCTGCCGCT
GGCGTCGTCATTGCGTTTGCAGGCCTATCGGTACGATATCAAAGGTCTGGTACTTAGCCCGTTTGGTAACGCCTCCTTTG
CTGGGGTGTATCGCGAGAAACAGGATGAGGTGAAAAAACCATGA

Upstream 100 bases:

>100_bases
AACCACACGGCCCTCATTACGACCCCTGAACACAAACGGTAATGGCAAATGTCATTAAATATTAGTAAGATAGCCTCTTT
ACATAAAATCCCTTAATATT

Downstream 100 bases:

>100_bases
TTATCTTCACCTTACGCCGCATTTTGTTATTGATTGTCACCTTGTTCCTGCTGACCTTCGTTGGCTTCAGCTTGAGCTAT
TTCACTCCACACGCGCCGTT

Product: peptide transport periplasmic protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAAFILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Sequences:

>Translated_547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAAFILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP
>Mature_547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAAFILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=547, Percent_Identity=99.4515539305302, Blast_Score=1125, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=35.7421875, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787052, Length=546, Percent_Identity=27.4725274725275, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1787762, Length=467, Percent_Identity=23.3404710920771, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=538, Percent_Identity=24.3494423791822, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=24.7983870967742, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789397, Length=528, Percent_Identity=23.8636363636364, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61609; Mature: 61609

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYL
CCCCHHHHEECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAAFILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHEEEEECCCCCCCCCCCEEECC
PAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRF
CCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALS
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHH
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHH
QDEVKKP
HHHCCCC
>Mature Secondary Structure
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYL
CCCCHHHHEECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAAFILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHEEEEECCCCCCCCCCCEEECC
PAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRF
CCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALS
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHH
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHH
QDEVKKP
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]