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The map label for this gene is prsA

Identifier: 30062731

GI number: 30062731

Start: 1257524

End: 1258471

Strand: Reverse

Name: prsA

Synonym: S1294

Alternate gene names: 30062731

Gene position: 1258471-1257524 (Counterclockwise)

Preceding gene: 30062732

Following gene: 30062730

Centisome position: 27.36

GC content: 52.95

Gene sequence:

>948_bases
GTGCCTGATATGAAGCTTTTTGCTGGTAACGCCACCCCGGTACTAGCACAACGTATTGCCAACCGCCTGTACACTTCACT
CGGCGACGCCGCTGTAGGTCGCTTTAGCGACGGCGAAGTCAGCGTACAAATTAACGAAAATGTACGCGGTGGTGATATTT
TCATCATCCAGTCCACTTGTGCCCCTACTAACGACAACCTGATGGAATTAGTCGTTATGGTTGATGCCCTGCGTCGTGCT
TCCGCAGGTCGTATCACCGCTGTTATCCCCTACTTTGGCTATGCGCGCCAGGACCGTCGCGTCCGTTCCGCTCGTGTACC
AATCACTGCGAAAGTGGTTGCAGACTTCCTCTCCAGCGTCGGTGTTGACCGTGTGCTGACAGTGGATCTGCACGCTGAAC
AGATTCAGGGTTTCTTCGACGTTCCGGTTGATAACGTATTTGGTAGCCCGATCCTGCTGGAAGACATGCTGCAGCTGAAT
CTGGATAACCCAATTGTGGTTTCTCCGGACATCGGCGGCGTTGTGCGTGCCCGCGCTATCGCTAAGCTGCTGAACGATAC
CGATATGGCAATCATCGACAAACGTCGTCCGCGTGCGAACGTTTCCCAGGTGATGCATATCATCGGTGACGTTGCAGGTC
GTGACTGCGTACTGGTCGATGATATGATCGACACTGGCGGTACGCTGTGTAAAGCTGCTGAAGCGCTGAAAGAACGTGGT
GCTAAACGTGTATTTGCGTACGCGACTCACCCGATCTTCTCTGGCAACGCGGCGAACAACCTGCGTAACTCTGTAATTGA
TGAAGTCGTTGTCTGCGATACCATTCCGCTGAGCGATGAAATCAAATCACTGCCGAACGTGCGTACTCTGACCCTGTCAG
GTATGCTGGCCGAAGCGATTCGTCGTATCAGCAACGAAGAATCGATCTCTGCCATGTTCGAACACTAA

Upstream 100 bases:

>100_bases
ATCGCGCTCTTTAATACACCGCCTGGAAAGGATCATGCCTGGCCCGCACAGTTTTCGGCAGATTCTTTCCACCAATGGAC
GCATGCCTGAGGTTCTTCTC

Downstream 100 bases:

>100_bases
TCGAACCCGGCTCAAAGACCCGCTGCGGCGGGTTTTTTTGTCTGTAATATCCATTTGTATGACCTATGCCTCCTTCACCT
GCCATTTAGTTGACAGATGA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH

Sequences:

>Translated_315_residues
MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH
>Mature_314_residues
PDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRAS
AGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNL
DNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGA
KRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=317, Percent_Identity=48.2649842271293, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI4506127, Length=318, Percent_Identity=48.4276729559748, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI84875539, Length=319, Percent_Identity=47.9623824451411, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=47.3186119873817, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=35.8600583090379, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=36.4431486880466, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI310128524, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310115209, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310118259, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310119946, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=99.6825396825397, Blast_Score=631, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=46.6876971608833, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=46.6876971608833, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=46.6876971608833, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=46.474358974359, Blast_Score=287, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=33.9233038348083, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=46.5189873417722, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=319, Percent_Identity=47.3354231974922, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=46.3492063492063, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=206, Percent_Identity=39.8058252427184, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=40, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355239, Length=317, Percent_Identity=47.0031545741325, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45551540, Length=339, Percent_Identity=43.952802359882, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.6478873239437, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.6478873239437, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=34.6478873239437, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=34.6478873239437, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=32.8877005347594, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=32.8877005347594, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=32.8877005347594, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34189; Mature: 34058

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTC
CCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
AKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC
>Mature Secondary Structure 
PDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTC
CCCEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
AKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]