Click here to switch to the map view.

The map label for this gene is insF1 [C]

Identifier: 30062589

GI number: 30062589

Start: 1104250

End: 1104729

Strand: Direct

Name: insF1 [C]

Synonym: S1134

Alternate gene names: 30062589

Gene position: 1104250-1104729 (Clockwise)

Preceding gene: 30062588

Following gene: 30062590

Centisome position: 24.01

GC content: 55.62

Gene sequence:

>480_bases
GTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGCTGGCAGAGAATGGCATCATCGTTGG
TCGTGACCGACTGGCACGTCTTCGTAAGGAGCTGAGGCTACGCTGTAAGCAGAAACGCAAGTTCAGAGCGACTACGAACC
CGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTACAGCACCAAATCAGGTCTGGGTGGCG
GACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCGG
CTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAGCGCCCACCTG
CCGGGCTAATCCACCACTCTGATCGAGGTTCCAGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTCTGGTCTGA

Upstream 100 bases:

>100_bases
TCAGGTATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGACAGAAAACAAAGTGATGAGC
GGCTAAAACTGGAGATTAAG

Downstream 100 bases:

>100_bases
AAACATCAATGTCGCGTAAAGGTAACTGTTACGACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAGC
CTGAGCCACTATCGTTTTAA

Product: IS600 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSSTAHTITGSYRSSLV

Sequences:

>Translated_159_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSSTAHTITGSYRSSLV
>Mature_158_residues
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVAD
LTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSSTAHTITGSYRSSLV

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=134, Percent_Identity=35.8208955223881, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788405, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787263, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1786753, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1786570, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1786490, Length=142, Percent_Identity=40.8450704225352, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1789981, Length=142, Percent_Identity=29.5774647887324, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 17951; Mature: 17820

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCCCHHCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
CCHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
KALFMALRSQRPPAGLIHHSDRGSSTAHTITGSYRSSLV
HHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCHHHCCC
>Mature Secondary Structure 
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCCHHCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
CCHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
KALFMALRSQRPPAGLIHHSDRGSSTAHTITGSYRSSLV
HHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]