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The map label for this gene is insD1 [C]

Identifier: 30062498

GI number: 30062498

Start: 1013019

End: 1013651

Strand: Direct

Name: insD1 [C]

Synonym: S1027

Alternate gene names: 30062498

Gene position: 1013019-1013651 (Clockwise)

Preceding gene: 161486516

Following gene: 30062501

Centisome position: 22.03

GC content: 56.4

Gene sequence:

>633_bases
ATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCTGGTGTCGCGTGCGCAGTTGCACGTCATTCTCAGACG
AACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTCTCCGTATACACCATGTTATCG
GAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGTTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATC
AATGCCAAACGTGTTTACCGGCTCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACCTGCTGTACCGCCATCGAAACG
GGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGTTCGAGTTCTGCTGTGATAACG
GAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGGGACTACCGGCGGCTTC
AACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGA
GTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTGTAA

Upstream 100 bases:

>100_bases
AACTCCAGCGCCTGCTCGGCAAGAAAACGATGGAAAATGAACTCCTCAAAGAAGCCGTTGAATATGGACGGGCAAAAAAG
TGGATAGCGCACGCGCCCTT

Downstream 100 bases:

>100_bases
TCTGGCGTTGCAGACGACCAATCTCTGGCAGTAATGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTA
TCTGCCCGTGGTTCAAGTTG

Product: IS2 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MARGWGVSLVSRCLLVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALFRRQAELDGMPAI
NAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAGTTGGF
NSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGL

Sequences:

>Translated_210_residues
MARGWGVSLVSRCLLVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALFRRQAELDGMPAI
NAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAGTTGGF
NSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGL
>Mature_209_residues
ARGWGVSLVSRCLLVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALFRRQAELDGMPAIN
AKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAGTTGGFN
SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGL

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1789424, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1789224, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788305, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787669, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786558, Length=210, Percent_Identity=97.6190476190476, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 24078; Mature: 23947

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGWGVSLVSRCLLVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYG
CCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCH
YRRVWALFRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCC
RWCSDGFEFCCDNGERLRVTFALDCCDREALHWAGTTGGFNSETVQDVMLGAVERRFGND
HHHHHHHHHHCCCCCEEEEEEEEECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCC
LPSSPVEWLTDNGSCYRANETRQFARMLGL
CCCCCHHHCCCCCCEEECHHHHHHHHHHCC
>Mature Secondary Structure 
ARGWGVSLVSRCLLVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYG
CCCCCHHHHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCH
YRRVWALFRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCC
RWCSDGFEFCCDNGERLRVTFALDCCDREALHWAGTTGGFNSETVQDVMLGAVERRFGND
HHHHHHHHHHCCCCCEEEEEEEEECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCC
LPSSPVEWLTDNGSCYRANETRQFARMLGL
CCCCCHHHCCCCCCEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA