Click here to switch to the map view.

The map label for this gene is ybjI [H]

Identifier: 30062326

GI number: 30062326

Start: 826889

End: 827677

Strand: Reverse

Name: ybjI [H]

Synonym: S0840

Alternate gene names: 30062326

Gene position: 827677-826889 (Counterclockwise)

Preceding gene: 30062327

Following gene: 30062325

Centisome position: 18.0

GC content: 46.01

Gene sequence:

>789_bases
ATGGATGGTACTTTCTTAAGCGATCAAAAAACCTATAACCGTGAGCGGTTTATGGCTCAGTATCAGCAAATGAAAGCACA
AGGCATTCGCTTTGTGGTCGCCAGCGGGAATCAATATTATCAGTTGATCTCTTTCTTCCCTGAAATTGCGAATGAAATTG
CCTTTGTGGCTGAAAACGGCGGCTGGGTAATGAGTGAAGGCAAAGATGTTTTTAATGGCGAGCTGTCGAAGGATGCGTTT
GCTACTGTCGTGGAACATTTGCTGACGCGCCCGGAAGTGGAAATTATTGCCTGCGGAAAAAATAGCGCCTATACACTCAA
AAAATATGACGATGCCATGAAAACGGTGGCGGAAATGTATTATCACCGTCTGGAATACGTCGATAACTTTGACAACTTAG
AAGATATTTTCTTTAAGTTTGGCCTGAATCTTTCCGATGAACTGATTCCACAAGTACAAAAAGCATTACATGAGGCCATC
GGCGATATTATGGTGCCAGTCCACACCGGCAACGGCAGCATCGATCTGATTATCCCCGGCGTACATAAAGCCAATGGCCT
TCGCCAACTGCAGAAATTATGGGGAATAGACGACAGCGAAGTGGTGGTCTTTGGCGATGGCGGTAACGATATTGAGATGC
TGCGTCAGGCAGGCTTTAGTTTTGCAATGGAAAATGCCGGCAGCGCAGTCGTCGCAGCGGCAAAATACCGGGCAGGCTAC
AATAACCGTGAAGGCGTACTGGATGTGATCGATAAAGTTCTTAAACACGAAGCGCCATTTGACCAATAA

Upstream 100 bases:

>100_bases
TTCTCGCGGCTATTGTCGCGAAAGCCGTCGCCAAACCCGATACCAAAACGCAGACGGCGATGGAGAATAGTTGATGAGCA
TTAAATTAATTGCGGTAGAC

Downstream 100 bases:

>100_bases
AAACGGCAGCACGCCCGGCAACACAATGCCTGAATGAAGGCTGCATAATTCGCGGAAATTAACCCGCTGCATTATTTCAG
GCGAAATGATTATGAAAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAF
ATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAI
GDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRAGY
NNREGVLDVIDKVLKHEAPFDQ

Sequences:

>Translated_262_residues
MDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAF
ATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAI
GDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRAGY
NNREGVLDVIDKVLKHEAPFDQ
>Mature_262_residues
MDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAF
ATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAI
GDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRAGY
NNREGVLDVIDKVLKHEAPFDQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=262, Percent_Identity=98.8549618320611, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787043, Length=261, Percent_Identity=51.7241379310345, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29344; Mature: 29344

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHEEEEECCC
GWVMSEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMY
CEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHH
YHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECC
VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRAGY
CCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCCEEHHHHHHCCC
NNREGVLDVIDKVLKHEAPFDQ
CCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHEEEEECCC
GWVMSEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMY
CEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHH
YHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECC
VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRAGY
CCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCCEEHHHHHHCCC
NNREGVLDVIDKVLKHEAPFDQ
CCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]