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The map label for this gene is gltK

Identifier: 30062159

GI number: 30062159

Start: 660823

End: 661497

Strand: Direct

Name: gltK

Synonym: S0650

Alternate gene names: 30062159

Gene position: 660823-661497 (Clockwise)

Preceding gene: 30062158

Following gene: 30062160

Centisome position: 14.37

GC content: 50.22

Gene sequence:

>675_bases
ATGTACGAGTTTGACTGGAGTTCCATTGTTCCTTCCCTGCCATATCTGCTCGACGGGCTGGTGATCACCCTGAAAATCAC
CGTCACGGCAGTCGTGATTGGTATTTTGTGGGGTACCATGCTGGCGGTGATGCGCTTGTCCAGCTTCGCGCCTGTTTCGT
GGTTTGCCAAAGCCTACGTTAACGTATTCCGCTCGATTCCTTTAGTCATGGTTTTGCTGTGGTTTTACTTGATTGTGCCG
GGATTTCTGCAAAATGTGCTGGGATTATCGCCAAAAAATGATATTCGTTTAATTTCGGCGATGGTGGCGTTTTCGATGTT
TGAAGCGGCCTACTATTCAGAGATTATCCGTGCCGGTATTCAAAGTATTTCCCGTGGTCAGTCGAGCGCCGCGCTGGCGT
TGGGGATGACTCACTGGCAGTCGATGAAACTGATTATTCTGCCGCAGGCTTTCCGCGCGATGGTGCCGCTGCTGCTCACT
CAGGGCATCGTACTGTTCCAGGATACCTCGCTGGTGTATGTGTTATCTCTGGCCGATTTCTTCCGTACCGCCTCAACCAT
TGGTGAGCGTGATGGTACGCAGGTCGAGATGATCCTGTTTGCCGGATTTGTTTATTTCGTTATTAGCCTTAGCGCGTCGT
TGTTGGTCAGCTACTTGAAAAGAAGGACAGCATAA

Upstream 100 bases:

>100_bases
CCATCACGCTTGCGTATGTGTTGATTAACGCTTTTATCATGCTGGTGATGACGCTGGTTGAACGTAAAGTTCGCCTGCCC
GGCAACATGGGGGGCAAATA

Downstream 100 bases:

>100_bases
TGATTACCCTGAAAAATGTTTCAAAATGGTATGGTCACTTTCAGGTGCTGACCGACTGCTCAACCGAAGTGAAAAAAGGC
GAAGTGGTGGTGGTTTGCGG

Product: glutamate/aspartate transport system permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Sequences:

>Translated_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
>Mature_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=99.5535714285714, Blast_Score=442, Evalue=1e-126,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=37.3831775700935, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=31.7307692307692, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787030, Length=223, Percent_Identity=30.4932735426009, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786874, Length=215, Percent_Identity=26.5116279069767, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787087, Length=217, Percent_Identity=26.7281105990783, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI48994930, Length=130, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788646, Length=210, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788645, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=25, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24931; Mature: 24931

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVSWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVSWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]