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The map label for this gene is entE

Identifier: 30062051

GI number: 30062051

Start: 525325

End: 526935

Strand: Direct

Name: entE

Synonym: S0514

Alternate gene names: 30062051

Gene position: 525325-526935 (Clockwise)

Preceding gene: 30062049

Following gene: 30062052

Centisome position: 11.42

GC content: 56.3

Gene sequence:

>1611_bases
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGGCAGTTGAGTTATCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCCTGTAGTTTGCGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAACTTTACATTACCTTTTTCGCGCTGCTGAAACTGGGCGTCGCGCCGGTGCTGGCGCTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTACGCCAGCCAGATTGAACCGGCGTTACTGATTGCCGATCGCCAGCATGCGCTATTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAACTGCACAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCAGAGGGTTTTACTGCCACGCCATCACCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGCACACCGAAACTGATCCCGCGCACTCATAACGACTATTACTACAGCGTGCGTCGTA
GCGCCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCTCGATCCCGGCGGCTCATAACTACGCTATGAGTTCG
CCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTTTGCTTCCCGTT
GATTGCAAAACATCAGGTTAACGTTACCGCGCTGGTGCCGCCGGCAGTCAGCCTGTGGTTGCAGGCGCTGGCTGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACGCTTGCAGCGCGTATT
CCCGCTGAGATTGGCTGCCAGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGACGATGAAGTGTGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAAGGGGAAGTTGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGTGGCTATTACAAAAGTCCGCAGCAC
AATGCCAGTGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGATATCGAAAACCTGCTGCTGAGCC
ATCCGGCAGTGATCTATGCCGCACTGGTGAGCATGGAAAATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Upstream 100 bases:

>100_bases
GGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTG
GATTGCATTAAGGAGCGAGG

Downstream 100 bases:

>100_bases
AGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTG
ACTGGGCCTTTGAACTGCAA

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCSIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Sequences:

>Translated_536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCSIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
>Mature_535_residues
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQLG
NVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGEH
NLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCSIPAAHNYAMSSP
GSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVVK
EPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=23.8188976377953, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=23.5294117647059, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI156151445, Length=435, Percent_Identity=24.367816091954, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI42544132, Length=503, Percent_Identity=22.2664015904573, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=23.1660231660232, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=23.1660231660232, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=23.5521235521236, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761345, Length=402, Percent_Identity=23.3830845771144, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI187761343, Length=402, Percent_Identity=23.3830845771144, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI115511026, Length=506, Percent_Identity=22.7272727272727, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=531, Percent_Identity=21.4689265536723, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI57165412, Length=384, Percent_Identity=25, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI57165410, Length=383, Percent_Identity=24.0208877284595, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8923896, Length=555, Percent_Identity=19.8198198198198, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=98.134328358209, Blast_Score=1087, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=30.6390977443609, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.2729124236253, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=24.7672253258845, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=23.8447319778189, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786801, Length=527, Percent_Identity=24.6679316888046, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786529, Length=527, Percent_Identity=22.9601518026565, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=21.9444444444444, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=505, Percent_Identity=23.3663366336634, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=26.3450834879406, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=544, Percent_Identity=26.1029411764706, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.015873015873, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=517, Percent_Identity=23.7911025145068, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=517, Percent_Identity=23.7911025145068, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=23.9923224568138, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=23.7623762376238, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=365, Percent_Identity=26.027397260274, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=529, Percent_Identity=23.2514177693762, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=26.8156424581006, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=22.2222222222222, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=22.0338983050847, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=518, Percent_Identity=24.1312741312741, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=544, Percent_Identity=23.3455882352941, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=544, Percent_Identity=23.3455882352941, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=539, Percent_Identity=22.8200371057514, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=22.037037037037, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581924, Length=521, Percent_Identity=23.2245681381958, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922652, Length=497, Percent_Identity=23.7424547283702, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=24.7169811320755, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=22.3908918406072, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=420, Percent_Identity=23.3333333333333, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667955, Length=565, Percent_Identity=22.4778761061947, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=541, Percent_Identity=22.365988909427, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648260, Length=272, Percent_Identity=26.4705882352941, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859661, Length=367, Percent_Identity=23.7057220708447, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=512, Percent_Identity=22.0703125, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=23.2620320855615, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648255, Length=500, Percent_Identity=22.6, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356441, Length=443, Percent_Identity=21.2189616252822, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281365686, Length=328, Percent_Identity=24.6951219512195, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20130357, Length=448, Percent_Identity=22.0982142857143, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.0440881763527, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 58852; Mature: 58721

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGEHNLQDAINHPAEGFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCSIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGEHNLQDAINHPAEGFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCSIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]