Click here to switch to the map view.

The map label for this gene is proY

Identifier: 30061898

GI number: 30061898

Start: 349628

End: 351001

Strand: Direct

Name: proY

Synonym: S0347

Alternate gene names: 30061898

Gene position: 349628-351001 (Clockwise)

Preceding gene: 30061897

Following gene: 30061899

Centisome position: 7.6

GC content: 52.55

Gene sequence:

>1374_bases
ATGGAAAGTAAGAATAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAC
CGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCG
CGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAG
GAAAACCTTGGCCCGCTGGCAGGTTACATTACCGGATGGACCTACTGTTTTGAAATCCTTATTGTCGCCATCGCCGATGT
GACCGCTTTTGGTATCTATATGGGTGTCTTGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCA
TTTGCGCCGTAAACCTGATGAGCGTGAAGGTCTTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATC
ATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCT
GTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTG
GGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATTCCGCGTGCGATTAACTCCGTGCCG
ATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCGG
TAGCCCGTTCGTACTGACGTTCCAGCATATGGGCATTACCTTTGCCGCCAGCATTCTTAACTTTGTTGTGCTGACCGCTT
CGCTGTCGGCAATTAACAGTGACGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCACCGAAA
ATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTA
TCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTATGGGTGTGGATTA
TGATCCTGCTGTCGCAAATCGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGT
GGGGTAGCAACGACCATCGGCGGGCTGATTTTCCTGCTCTTTATTATCGGGTTGATTGGTTATCACCCGGATACGCGTAT
CTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGG
CTGAAAACCAATAA

Upstream 100 bases:

>100_bases
TCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTG
TGTTGATGGGTTAGGAATTG

Downstream 100 bases:

>100_bases
TACTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCC
CAAATATCCTTCAGATGATG

Product: putative proline-specific permease

Products: Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ

Sequences:

>Translated_457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
>Mature_457_residues
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQ
ENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPK
IFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPG
GVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of proline [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI110347453, Length=351, Percent_Identity=24.5014245014245, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI181337167, Length=338, Percent_Identity=24.8520710059172, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI258645169, Length=431, Percent_Identity=22.5058004640371, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4507047, Length=364, Percent_Identity=23.6263736263736, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI258614003, Length=429, Percent_Identity=22.1445221445221, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI258614005, Length=429, Percent_Identity=22.1445221445221, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786602, Length=457, Percent_Identity=99.781181619256, Blast_Score=909, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=452, Percent_Identity=44.6902654867257, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1786302, Length=453, Percent_Identity=43.2671081677704, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI48994972, Length=437, Percent_Identity=45.766590389016, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790653, Length=454, Percent_Identity=40.9691629955947, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI87081915, Length=404, Percent_Identity=37.8712871287129, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI87081708, Length=463, Percent_Identity=36.0691144708423, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788480, Length=406, Percent_Identity=36.9458128078818, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1789017, Length=433, Percent_Identity=34.1801385681293, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI87081869, Length=235, Percent_Identity=23.8297872340426, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87082023, Length=331, Percent_Identity=24.4712990936556, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786694, Length=432, Percent_Identity=23.8425925925926, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=425, Percent_Identity=23.2941176470588, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=360, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533459, Length=401, Percent_Identity=23.1920199501247, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540018, Length=465, Percent_Identity=23.4408602150538, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=444, Percent_Identity=33.5585585585586, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6320772, Length=404, Percent_Identity=34.4059405940594, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6324990, Length=440, Percent_Identity=31.5909090909091, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324924, Length=407, Percent_Identity=31.4496314496315, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322892, Length=475, Percent_Identity=30.1052631578947, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324059, Length=410, Percent_Identity=32.9268292682927, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319824, Length=432, Percent_Identity=27.7777777777778, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319542, Length=430, Percent_Identity=27.6744186046512, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=31.8295739348371, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321629, Length=479, Percent_Identity=26.9311064718163, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320717, Length=403, Percent_Identity=27.7915632754342, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320251, Length=435, Percent_Identity=26.8965517241379, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319543, Length=405, Percent_Identity=27.4074074074074, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321053, Length=472, Percent_Identity=26.0593220338983, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319608, Length=459, Percent_Identity=25.9259259259259, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322967, Length=390, Percent_Identity=25.1282051282051, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324981, Length=397, Percent_Identity=25.1889168765743, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320364, Length=511, Percent_Identity=24.853228962818, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221512776, Length=410, Percent_Identity=21.4634146341463, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24666159, Length=410, Percent_Identity=21.4634146341463, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161077963, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24667468, Length=343, Percent_Identity=22.4489795918367, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50143; Mature: 50143

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVLFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSVLLAYIIGGIAAYIIMR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIVAIADVTAFGIYMGVLFPT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATIIIMIVAGFGIIIWGIGNGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
PTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVP
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHH
MRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASLATFATVWVWIMILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHC
YHPDTRISLYVGFAWIVVLLIGWMFKRRHDRQLAENQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]