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The map label for this gene is aceF

Identifier: 30061681

GI number: 30061681

Start: 123446

End: 125326

Strand: Direct

Name: aceF

Synonym: S0114

Alternate gene names: 30061681

Gene position: 123446-125326 (Clockwise)

Preceding gene: 30061680

Following gene: 30061682

Centisome position: 2.68

GC content: 54.55

Gene sequence:

>1881_bases
ATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAA
AGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCATCTCCGCAGGCGGGTATCG
TTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCA
GCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCCGCGGCTGCGGCGGCAAAAGA
CGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTG
AACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGTACCGTGAAAGAGATC
AAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGC
TCCGGCGGCTAAACAGGAAGCGACTCCGGCAGCGACCCCTGCACCAGCAGCTGGCGTGAAAGAAGTTAACGTTCCGGATA
TCGGCGGTGACGAAGTTGAAGTGACCGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATTACC
GTAGAAGGCGATAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGA
TAAAGTGAAAACTGGTTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACAGGAAG
CGGCAGCGCCGGCTCCGGCAGCAAAAGCTGAAGCTCCGGCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGAC
GCTTACGTTCACGCGACCCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGTGAAGGGCACTGG
CCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCTTACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCGG
CGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGCGAAATCGAAGAAGTGGAA
CTGGGCCGTATCCAGAAAATTTCTGGTGCTAACCTGAGCCGTAACTGGGTGATGATCCCGCATGTTACCCACTTCGACAA
AACCGATATCACCGAGCTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCA
CCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGAC
GGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATT
CAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGC
TGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCATCGGCGGCCTGGGTACTACCCACTTCGCGCCGATT
GTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTGGAATGGTAAAGAGTTCGTGCC
GCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCA
TTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAA

Upstream 100 bases:

>100_bases
GTGGCGAAATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTG
GCGTAAGAGGTAAAAGAATA

Downstream 100 bases:

>100_bases
GTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCG
GTTGCCGTTTGTTGTTTAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGA
ADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
VEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAEND
AYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVE
LGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSED
GQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPI
VNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Sequences:

>Translated_626_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGA
ADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
VEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAEND
AYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVE
LGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSED
GQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPI
VNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
>Mature_625_residues
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAA
DAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIK
VNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
EGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDA
YVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVEL
GRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDG
QRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIV
NAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=30.7522123893805, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI31711992, Length=318, Percent_Identity=33.9622641509434, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=442, Percent_Identity=27.1493212669683, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI203098753, Length=442, Percent_Identity=27.1493212669683, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=33.6585365853659, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=36.094674556213, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786305, Length=630, Percent_Identity=98.5714285714286, Blast_Score=1047, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=31.3432835820896, Blast_Score=172, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=409, Percent_Identity=30.5623471882641, Blast_Score=182, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=413, Percent_Identity=28.0871670702179, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=36.231884057971, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=29.0322580645161, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=27.0731707317073, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=349, Percent_Identity=30.0859598853868, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=30.8962264150943, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.953488372093, Blast_Score=119, Evalue=9e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65846; Mature: 65715

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVS
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNVGDKVSTGSLIMVFEVA
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEEC
GEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCHHHCCCEEEE
VEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPA
EECCCCCEECCCCHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCHHHCCCCCCC
AKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAY
CCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHH
VKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIP
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEEEEC
HVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSED
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHCC
GQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQ
CCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCC
GGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHR
CCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHEEEEEEECCCCE
VIDGADGARFITIINNTLSDIRRLVM
EEECCCCCEEEEEEHHHHHHHHHHHC
>Mature Secondary Structure 
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVS
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEV
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKVNVGDKVSTGSLIMVFEVA
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEEC
GEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCHHHCCCEEEE
VEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPA
EECCCCCEECCCCHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCHHHCCCCCCC
AKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAY
CCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHH
VKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIP
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCEEEEC
HVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSED
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHCC
GQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQ
CCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCC
GGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHR
CCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHEEEEEEECCCCE
VIDGADGARFITIINNTLSDIRRLVM
EEECCCCCEEEEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]