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The map label for this gene is yaaF

Identifier: 30061598

GI number: 30061598

Start: 26209

End: 27123

Strand: Direct

Name: yaaF

Synonym: S0029

Alternate gene names: 30061598

Gene position: 26209-27123 (Clockwise)

Preceding gene: 30061597

Following gene: 30061599

Centisome position: 0.57

GC content: 55.74

Gene sequence:

>915_bases
ATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGA
ACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGC
ATTTCTGGAATGCGGAGATTCCGCTCGCCCAGGGAGCCGCTGTGCCGCTGGTACGCGCACCGCGTGATGCAGCATCTGTG
CACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCACTCGGGATACCGGCGTTTCTGGCGAT
TCGGGATGCCCTGATGCGTGCACCAGAGCCCGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTT
TACAATGCCCGGAATGTAAACCGTATATTCGTCGTCTGGTGATCATGGGCGGTTCTGCTGGTCGCGGCAACTGTACACCA
AATGCCGAGTTTAATATCGCTGCCGATCCAGAAGCGGCTGCCTGTGTCTTCCGTAGCGGCATCGAAATTGTCATGTGCGG
TCTGGATGTCACCAATCAGGCAATATTAACCCCTGACTATCTCGCTACTTTACCGGAGTTGAATCGCACCGGAAAAATGC
TCCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGTCTGCGAATGCACGATCTCTGCGCCATCGCCTGG
CTGGTGCGCCCGGACTTATTCACCCTCAAGCCGTGTTTTGTCGCGGTAGAAACTCAAGGCGAATTTACCTCAGGCACGAC
GGTGGTTGATATCGACGGTTGCCTGGGCAAACCAGCCAATGTACAGGTGGCATTGGATCTGAATGTAAAAGGCTTCCAAC
AGTGGGTGGCTGAGGTGCTGGCTCTGGTGCCGTAA

Upstream 100 bases:

>100_bases
AAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCG
GCATTTTTTATGAAGAAAAC

Downstream 100 bases:

>100_bases
CCTGTCACATGTTATCGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTC
TAATTATCAGCGTTTTTGGC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLNVKGFQQWVAEVLALVP

Sequences:

>Translated_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLNVKGFQQWVAEVLALVP
>Mature_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLNVKGFQQWVAEVLALVP

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=302, Percent_Identity=98.6754966887417, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=44.6601941747573, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788486, Length=305, Percent_Identity=39.0163934426229, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=33.5106382978723, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=32.1052631578947, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=339, Percent_Identity=29.2035398230088, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24641839, Length=196, Percent_Identity=33.1632653061224, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583915, Length=191, Percent_Identity=33.5078534031414, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641837, Length=189, Percent_Identity=33.8624338624339, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32609; Mature: 32609

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLNVKGFQQWVAEVL
HHCCCCEEECEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALVP
HHCC
>Mature Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLNVKGFQQWVAEVL
HHCCCCEEECEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALVP
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA