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The map label for this gene is nhaR

Identifier: 30061588

GI number: 30061588

Start: 17371

End: 18270

Strand: Direct

Name: nhaR

Synonym: S0019

Alternate gene names: 30061588

Gene position: 17371-18270 (Clockwise)

Preceding gene: 30061587

Following gene: 30061592

Centisome position: 0.38

GC content: 47.44

Gene sequence:

>900_bases
ATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGC
GCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCA
AGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAA
ATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCT
GGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGC
TGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTG
TTCTCCGTGAGAATTGGCGAATGTGGCGTGAGTTTCTGGTGTACAAATCCACCACCAGAAAAACCGTTCCCGGCTTGTCT
GGAAGAACGGCGACTTTTGATTCCTGGGCGACGTTCAATGTTAGGGCGCAAATTGCTTAACTGGTTTAACTCCCAGGGAT
TAAACGTAGAAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTT
GCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGA
GTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTT
TTAGTCCAGCGGTGCGTTAA

Upstream 100 bases:

>100_bases
GCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGT
TATCAGGGAGAGAAATGAGC

Downstream 100 bases:

>100_bases
TCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGC
CCGCTTGAGACATTGTTTCC

Product: transcriptional activator NhaR

Products: NA

Alternate protein names: Na(+)/H(+) antiporter regulatory protein

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQE
MLDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFV
APTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR

Sequences:

>Translated_299_residues
MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQE
MLDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFV
APTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR
>Mature_298_residues
SHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEM
LDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGLF
SVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFVA
PTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR

Specific function: Plays a role in the positive regulation of nhaA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786202, Length=299, Percent_Identity=100, Blast_Score=626, Evalue=0.0,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=24.5967741935484, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAR_ECOLI (P0A9G2)

Other databases:

- EMBL:   X04382
- EMBL:   L24072
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_000684.1
- RefSeq:   NP_414561.1
- ProteinModelPortal:   P0A9G2
- IntAct:   P0A9G2
- STRING:   P0A9G2
- PRIDE:   P0A9G2
- EnsemblBacteria:   EBESCT00000000506
- EnsemblBacteria:   EBESCT00000000507
- EnsemblBacteria:   EBESCT00000016551
- GeneID:   944757
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0019
- KEGG:   eco:b0020
- EchoBASE:   EB1070
- EcoGene:   EG11078
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008345
- HOGENOM:   HBG685425
- OMA:   LCLTPQT
- ProtClustDB:   PRK11062
- BioCyc:   EcoCyc:PD00474
- Genevestigator:   P0A9G2
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34067; Mature: 33935

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEP
CCCCCCCEEEEEEEEEECCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
PIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGLFSVRIGECGVSFWCTNPPPE
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
KPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFV
CCCHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHEEE
APTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
SHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEERLQGKLFKRKGRGLEP
CCCCCCEEEEEEEEEECCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADALSKRLVSSVLNAAVVEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCC
PIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGLFSVRIGECGVSFWCTNPPPE
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCC
KPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGAMHNAIFV
CCCHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHEEE
APTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYSALFSPAVR
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2429258; 1630901; 9278503; 3413113; 1316901; 8168494