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The map label for this gene is ilvI

Identifier: 161486550

GI number: 161486550

Start: 82957

End: 84681

Strand: Direct

Name: ilvI

Synonym: S0074

Alternate gene names: 161486550

Gene position: 82957-84681 (Clockwise)

Preceding gene: 161486551

Following gene: 30061643

Centisome position: 1.8

GC content: 52.81

Gene sequence:

>1725_bases
ATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGG
AGGCGCAGTCCTTGATATTTACGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGG
CGGTGCATATGGCCGATGGCCTGGCGCGTGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACC
AATGCGATTACCGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTT
GATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGC
AAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTGGTCGTTGAT
TTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGCCCTGAGTCGGTCAGTATGCGTTCTTACAATCC
CACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAG
GCGGTGGGGCAATCATGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCA
TTGATGGGAATGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAA
TATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGT
ACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATT
GTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCCTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGAT
CCGCGACTGGTGGCAGCAGATTGAACAGTGGCGCGCTCTTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAAC
CGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACTAATGGGGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATG
TTTGCTGCGCTTTATTATCCATTCGACAAACCGCGTCGCTGGATCAATTCCGGCGGTCTCGGCACGATGGGTTTTGGTTT
ACCTGCGGCACTGGGCGTCAAAATGGCGTTGCCAGAAGAAACCGTGGTTTGCGTCACTGGCGACGGCAGTATTCAGATGA
ACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTACTGGTGGTGAATCTCAATAACCGCTATCTGGGGATG
GTGAAACAGTGGCAGGACATGATCTATTCCGGCCGTCATTCACAATCTTATATGCAATCACTACCCGATTTCGTCCGTCT
GGCGGAAGCCTATGGTCACGTCGGGATCCAGATCTCTCATCCGCATGAGCTGGAAAGCAAACTTAGCGAGGCGCTGGAAC
AGGTGCGCAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGTGAGCACGTCTACCCGATGCAGATTCGCGGG
GGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGA

Upstream 100 bases:

>100_bases
TTATTACCCTGCGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCC
GTCAAACAGTGAGGCAGGCC

Downstream 100 bases:

>100_bases
TTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGA
GGCTACAACATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Sequences:

>Translated_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT
>Mature_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=27.9646017699115, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=25.8620689655172, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=98.9547038327526, Blast_Score=1174, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=39.2605633802817, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=33.0388692579505, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=29.6229802513465, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.8201438848921, Blast_Score=172, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=27.4509803921569, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=27.4509803921569, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=26.2350936967632, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.7101200686106, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=25.7796257796258, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922626, Length=508, Percent_Identity=28.740157480315, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62877; Mature: 62877

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVCPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCCCCCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHCCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAL
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTNGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVCPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCCCCCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHCCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAL
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTNGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]