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The map label for this gene is hsdS [C]

Identifier: 29349951

GI number: 29349951

Start: 5963411

End: 5964556

Strand: Reverse

Name: hsdS [C]

Synonym: BT_4543

Alternate gene names: 29349951

Gene position: 5964556-5963411 (Counterclockwise)

Preceding gene: 29349954

Following gene: 29349949

Centisome position: 95.27

GC content: 37.0

Gene sequence:

>1146_bases
ATGAGCGATGTTCCCCATTTGAGATTTCCGGAGTTTAGTGGAGAGTGGGAAGAGCATACGCTTTCTGAATATTTGGAGTT
CAAGAATGGTCTTAATCCAGACGCTAAACGTATTGGAAGTGGACTACCATTCATTTCGGTGATGGATATTCTTTCAGAAG
GAGTTATCAATTATGACAATATTCGTGGCAAGGTCAATGCTACTGAAAAAGAAATAGAATGTTTCGGAGTGAAAGACGGA
GATTTGCTATTCCAACGTAGCTCAGAAACATTGGAGGATGTTGGACGAGCTAATGTGTACATGGATAATCGTACCGCCAT
TTATGGAGGCTTTGTTATTCGTGGTCGTAAAATCGGCAATTATGATCCTTTGTTCTTTAAGTATTTGCTTGCTACGCCAT
TAGCGCGCAAAAGAACTTGCAGAATGGGAGCAGGAGCACAACATTTCAATATTGGGCAGGAAGGATTATCCAAAATATCA
TTATATTTTCCATCAATTGAGGAACAGAGAAAAATAGCTGAATTTCTATCTCTCATTGACGAACGAATAGCAACCCAAAA
CAAAATCATTGAGGATTTGAAAAAACTAAAGTCCGCAATTAGTTTGAATGTACTTCATTCCGATAAATGGGAACAGTTCA
AAATCAAAGATATCGCCCAAATAGGGCGTGGTAGAGTTATTAGTTCCATAGAAATTGGCCAGCAAAAGAGCCCTACATAT
CCAGTATATTCCTCTCAAACATCAAATGATGGTATTATGGGCTACCTTGACGATTATATGTTTGAAGGTGAATACATCAG
TTGGACCACTGACGGTGCAAATGCAGGAACTGTATTTTATCGTAATGGAAAATTTAATTGCACAAATGTTTGTGGTTTAT
TAAAACTTAGAAAAGAGTTTGATACTCATTTTGTTTCTTTGGTTTTGGCGGAAGCGACAAAGAAATATGTCTCAATAAAC
TTAGCTAACCCAAAACTTATGAACAATACAATGGGTAACATTCAAATACGTCTGCCCAAATTAGAAGAGCAAAAAAGAAT
ATCCATCGTTTTCAGAGTGTTACAAAGGCTATGGACTGTACATAATAGTTTGCTTACTGAGTATACAAAGCAGGAACAAT
ATTTGCTCTCACAGATGTTCATATAA

Upstream 100 bases:

>100_bases
GGGTCGCTTGTACTCTTTCCAAGCTACCGCAATTTCTCTAATTGTTTTGTTGCTCATATTATTTCATTTAATGATAAATG
TCTATAATTAGAGAAATATA

Downstream 100 bases:

>100_bases
ACATTTGCGAGAGCAGGTACTGTTTTTCATCCTCATATCTTACCGAGGTATTTTCTTCAAGAGCTAACTTATTATCTAAT
GAAGTCATTAGAGAGGCTAT

Product: putative type I restriction enzyme specificity protein

Products: NA

Alternate protein names: S.MpnORFGP; Type I restriction enzyme specificity protein MPN_285; S protein [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSDVPHLRFPEFSGEWEEHTLSEYLEFKNGLNPDAKRIGSGLPFISVMDILSEGVINYDNIRGKVNATEKEIECFGVKDG
DLLFQRSSETLEDVGRANVYMDNRTAIYGGFVIRGRKIGNYDPLFFKYLLATPLARKRTCRMGAGAQHFNIGQEGLSKIS
LYFPSIEEQRKIAEFLSLIDERIATQNKIIEDLKKLKSAISLNVLHSDKWEQFKIKDIAQIGRGRVISSIEIGQQKSPTY
PVYSSQTSNDGIMGYLDDYMFEGEYISWTTDGANAGTVFYRNGKFNCTNVCGLLKLRKEFDTHFVSLVLAEATKKYVSIN
LANPKLMNNTMGNIQIRLPKLEEQKRISIVFRVLQRLWTVHNSLLTEYTKQEQYLLSQMFI

Sequences:

>Translated_381_residues
MSDVPHLRFPEFSGEWEEHTLSEYLEFKNGLNPDAKRIGSGLPFISVMDILSEGVINYDNIRGKVNATEKEIECFGVKDG
DLLFQRSSETLEDVGRANVYMDNRTAIYGGFVIRGRKIGNYDPLFFKYLLATPLARKRTCRMGAGAQHFNIGQEGLSKIS
LYFPSIEEQRKIAEFLSLIDERIATQNKIIEDLKKLKSAISLNVLHSDKWEQFKIKDIAQIGRGRVISSIEIGQQKSPTY
PVYSSQTSNDGIMGYLDDYMFEGEYISWTTDGANAGTVFYRNGKFNCTNVCGLLKLRKEFDTHFVSLVLAEATKKYVSIN
LANPKLMNNTMGNIQIRLPKLEEQKRISIVFRVLQRLWTVHNSLLTEYTKQEQYLLSQMFI
>Mature_380_residues
SDVPHLRFPEFSGEWEEHTLSEYLEFKNGLNPDAKRIGSGLPFISVMDILSEGVINYDNIRGKVNATEKEIECFGVKDGD
LLFQRSSETLEDVGRANVYMDNRTAIYGGFVIRGRKIGNYDPLFFKYLLATPLARKRTCRMGAGAQHFNIGQEGLSKISL
YFPSIEEQRKIAEFLSLIDERIATQNKIIEDLKKLKSAISLNVLHSDKWEQFKIKDIAQIGRGRVISSIEIGQQKSPTYP
VYSSQTSNDGIMGYLDDYMFEGEYISWTTDGANAGTVFYRNGKFNCTNVCGLLKLRKEFDTHFVSLVLAEATKKYVSINL
ANPKLMNNTMGNIQIRLPKLEEQKRISIVFRVLQRLWTVHNSLLTEYTKQEQYLLSQMFI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 43559; Mature: 43428

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVPHLRFPEFSGEWEEHTLSEYLEFKNGLNPDAKRIGSGLPFISVMDILSEGVINYDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
IRGKVNATEKEIECFGVKDGDLLFQRSSETLEDVGRANVYMDNRTAIYGGFVIRGRKIGN
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCC
YDPLFFKYLLATPLARKRTCRMGAGAQHFNIGQEGLSKISLYFPSIEEQRKIAEFLSLID
CCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHH
ERIATQNKIIEDLKKLKSAISLNVLHSDKWEQFKIKDIAQIGRGRVISSIEIGQQKSPTY
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
PVYSSQTSNDGIMGYLDDYMFEGEYISWTTDGANAGTVFYRNGKFNCTNVCGLLKLRKEF
CEECCCCCCCCCEEHHHHHEECCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
DTHFVSLVLAEATKKYVSINLANPKLMNNTMGNIQIRLPKLEEQKRISIVFRVLQRLWTV
HHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
HNSLLTEYTKQEQYLLSQMFI
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVPHLRFPEFSGEWEEHTLSEYLEFKNGLNPDAKRIGSGLPFISVMDILSEGVINYDN
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
IRGKVNATEKEIECFGVKDGDLLFQRSSETLEDVGRANVYMDNRTAIYGGFVIRGRKIGN
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCC
YDPLFFKYLLATPLARKRTCRMGAGAQHFNIGQEGLSKISLYFPSIEEQRKIAEFLSLID
CCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHH
ERIATQNKIIEDLKKLKSAISLNVLHSDKWEQFKIKDIAQIGRGRVISSIEIGQQKSPTY
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
PVYSSQTSNDGIMGYLDDYMFEGEYISWTTDGANAGTVFYRNGKFNCTNVCGLLKLRKEF
CEECCCCCCCCCEEHHHHHEECCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
DTHFVSLVLAEATKKYVSINLANPKLMNNTMGNIQIRLPKLEEQKRISIVFRVLQRLWTV
HHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
HNSLLTEYTKQEQYLLSQMFI
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]