Click here to switch to the map view.

The map label for this gene is hsdS [H]

Identifier: 29349931

GI number: 29349931

Start: 5942349

End: 5943533

Strand: Reverse

Name: hsdS [H]

Synonym: BT_4523

Alternate gene names: 29349931

Gene position: 5943533-5942349 (Counterclockwise)

Preceding gene: 29349932

Following gene: 29349929

Centisome position: 94.94

GC content: 37.38

Gene sequence:

>1185_bases
ATGCCACATTATGAGAACGTGCCGTTTGAAATACCGAATTCATGGGTGTGGACAACGATTGAAGAAATATGCTCAAAGAT
AGGTTCCGGTAGCACGCCAAGGGGAAGTAACTATTCTGCGAACGGGATCCCTTTTTTCCGCTCGCAAAATGTATATAATG
ACAGGCTTGTTTATGATGATATCAAATATATTTCCGAAGAAGTTCATCAGAAGATGAAAGGTACGGAGGTACTTGCTAAC
GACTTGTTGTTGAATATTACTGGTGGTTCATTGGGAAGATGTGCTGTAGTTCCTGCTGATTTTAATTGTGGTAATGTTAG
TCAACACGTTTGTATTATGCGTTCTGTTTTAGTTGAACCTGAATATTTCCATGCGCTAGTTTTATCTTCATACTTCGCTA
AATCTATGAAGATTACGGGTAGTGGTCGTGAAGGATTACCAAAATACAGTTTAGAACAAATGGCTTTTCCTTTACCACCT
CTTAGTGAGCAACAGCGCATTGTAATGGAAATAGAGAAATTGTTTGCTTTAATCGACCAAATAGAACATAGCAAAGTAAA
TTTGCAAACCATCATAAAGCAAACCAAGAGCAAGATTCTTGATCTTGCCATTCACGGAAAACTTGTGCCGCAAGACCCAA
ACGATGAACCTGCTATCGAATTGTTGAAACGTATAAATCCCGACTTTACACCTTGTGATAACGGGCATTATACGCAGTTG
CCTGATGGATGGACGTTTTGTAGGCTTGACCAAATTATAGGTTATGAACAGTCTACAGCTTATATTGTAGAATCGACAGC
TTATGATGACTCTTATTCAACCCCAGTTCTGACAGCCGGTAAATCATTTATTATTGGTTACACTAACGAGGCTACTGGTA
TATACTCGAATCTTCCTTGTATAATCTTTGATGATTTTACAACTGATTCGAAACTAGTTGATTTCCCTTTTAAGGTAAAG
TCATCTGCAATGAAGATTCTAAAAGTACATAAGGACATTGAAGTTGATTATGTGGCAATGTTTATGAGCATAACCAAATT
GGTAGGTGACACCCATAAACGATATTGGATTTCTGAATACTCAAAATTAGAGATACCAATACCATCGAAGGCAGAACAAA
AGCGAATTATTCATGCAATCCATGGAATCTTTACACAATTAGATTTAATAATGGAGAGCCTATAA

Upstream 100 bases:

>100_bases
GAACAACCCATATCCTATTTCAAGGATAAAGAATATCTATCATTCAAGGAGGTTGCAACTTTATTGGGGCTGAGCAAACA
AGCCGTATATAAAATGGTAT

Downstream 100 bases:

>100_bases
ACTCTCCATTATTAAATCTAAAGACATAAAAGCCTTGGTTATAGCCTTTATAATCCTTTGTTGTTCTTTGTAAGGTGGAA
TAGGAATTTCTATTGCTTTG

Product: putative type I restriction enzyme EcoR124II protein

Products: NA

Alternate protein names: S.EcoR124II; Type I restriction enzyme EcoR124II specificity protein; S protein [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDDIKYISEEVHQKMKGTEVLAN
DLLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEPEYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPP
LSEQQRIVMEIEKLFALIDQIEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQL
PDGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPCIIFDDFTTDSKLVDFPFKVK
SSAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEYSKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL

Sequences:

>Translated_394_residues
MPHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDDIKYISEEVHQKMKGTEVLAN
DLLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEPEYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPP
LSEQQRIVMEIEKLFALIDQIEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQL
PDGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPCIIFDDFTTDSKLVDFPFKVK
SSAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEYSKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL
>Mature_393_residues
PHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDDIKYISEEVHQKMKGTEVLAND
LLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEPEYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPPL
SEQQRIVMEIEKLFALIDQIEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQLP
DGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPCIIFDDFTTDSKLVDFPFKVKS
SAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEYSKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 44544; Mature: 44412

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDD
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHH
IKYISEEVHQKMKGTEVLANDLLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEP
HHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
EYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPPLSEQQRIVMEIEKLFALIDQ
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQL
HHCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PDGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPC
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCE
IIFDDFTTDSKLVDFPFKVKSSAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEY
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
SKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDD
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHH
IKYISEEVHQKMKGTEVLANDLLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEP
HHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
EYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPPLSEQQRIVMEIEKLFALIDQ
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQL
HHCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PDGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPC
CCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCE
IIFDDFTTDSKLVDFPFKVKSSAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEY
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
SKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]