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The map label for this gene is mltD [H]

Identifier: 29349895

GI number: 29349895

Start: 5903417

End: 5904418

Strand: Reverse

Name: mltD [H]

Synonym: BT_4487

Alternate gene names: 29349895

Gene position: 5904418-5903417 (Counterclockwise)

Preceding gene: 29349896

Following gene: 29349894

Centisome position: 94.31

GC content: 47.8

Gene sequence:

>1002_bases
ATGACTAAAAGAAAACAGAAAATGAAGAAATTTACCAAGATCAACTACATTCTAACCTTTATATTAGTGTTCTGCATCGG
AGCTACCCTGCCTATTCTGATCGGTAGCAGTCATGTAGACGAACAGCATTCCGCCAAATCGGAAGTACCTTATTGCGTCA
CCTCACCTACCGTTCCCGAGCAGGTGACTTTTGACGGAGAGACGATCGACCTCCGCCGTTACGACCGCCGCGAGCGGATG
GACCGGGAGATGATGGCGTTCACCTATATGCACTCCACCACCATGCTGCTAATTAAGCGTGCGAACCGTTACTTCCCAAT
TATAGAACCGATTCTGAAAGCGAACGGTATCCCCGACGATTTCAAATATCTGATGGTCATCGAAAGTAACCTCAACAACA
TAGCCCGCTCTCCGGCCGGTGCAGCCGGATTATGGCAATTCATGCCTGCTACCGGACGGGAATTCGGACTCGAAGTAAAT
GAGAACGTAGACGAACGTTATCACATCGAAAAAGCAACGGTAGCCGCCTGCAAATATTTCAAACAGGCTTATGCCAAATA
CGGAGACTGGATGGCTGTATCTGCCGCCTATAATGCCGGACAAGGACGAATTTCCTCTCAACTGGACCAGCAACTTGCCA
GCCACGCAATGGACTTATGGCTGGTAGAAGAGACTTCCCGCTATATGTTCCGCCTGCTGGCAGTCAAAGAAATATTCAAG
AATCCTCAACGATATGGATTCCTGCTGAAAAAGGAACACCTGTACCCGCCTATCCCCTACAAAGAGATAACGGTCACCAC
CCCCATTGCCAACTTAAGCGATTTTGCCAAACAGCAGGGAATCACATACGCCCAACTTCGAGATGCGAACCCTTGGCTAC
GCGAACAAACCCTAAAGAACAGGACCGGAAAAACATACGTTCTCCAGATACCTACACAAGAGGGAATGTACTATGATCCA
AAGAAGACGGTTGCATATAACAAGCACTGGGTAATAGATTAA

Upstream 100 bases:

>100_bases
CCTGTCCCCGTTGCGGAGAAACCATTGTCAAGGAGAATTATCTTGGCGGTAGCATCTATTACTGCCGTGGTTGCCAGATA
TTGGAATAAGAACCAATCAA

Downstream 100 bases:

>100_bases
TGTAATAGGTAATAAGTAGCAGGTAATAAGTAATAGGTAAAATACAGGTTATTGGGATATTGGCCTGTCAACTGATTATT
CTCATGAAACTAAAAGAAAG

Product: putative membrane-bound lytic murein transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTKRKQKMKKFTKINYILTFILVFCIGATLPILIGSSHVDEQHSAKSEVPYCVTSPTVPEQVTFDGETIDLRRYDRRERM
DREMMAFTYMHSTTMLLIKRANRYFPIIEPILKANGIPDDFKYLMVIESNLNNIARSPAGAAGLWQFMPATGREFGLEVN
ENVDERYHIEKATVAACKYFKQAYAKYGDWMAVSAAYNAGQGRISSQLDQQLASHAMDLWLVEETSRYMFRLLAVKEIFK
NPQRYGFLLKKEHLYPPIPYKEITVTTPIANLSDFAKQQGITYAQLRDANPWLREQTLKNRTGKTYVLQIPTQEGMYYDP
KKTVAYNKHWVID

Sequences:

>Translated_333_residues
MTKRKQKMKKFTKINYILTFILVFCIGATLPILIGSSHVDEQHSAKSEVPYCVTSPTVPEQVTFDGETIDLRRYDRRERM
DREMMAFTYMHSTTMLLIKRANRYFPIIEPILKANGIPDDFKYLMVIESNLNNIARSPAGAAGLWQFMPATGREFGLEVN
ENVDERYHIEKATVAACKYFKQAYAKYGDWMAVSAAYNAGQGRISSQLDQQLASHAMDLWLVEETSRYMFRLLAVKEIFK
NPQRYGFLLKKEHLYPPIPYKEITVTTPIANLSDFAKQQGITYAQLRDANPWLREQTLKNRTGKTYVLQIPTQEGMYYDP
KKTVAYNKHWVID
>Mature_332_residues
TKRKQKMKKFTKINYILTFILVFCIGATLPILIGSSHVDEQHSAKSEVPYCVTSPTVPEQVTFDGETIDLRRYDRRERMD
REMMAFTYMHSTTMLLIKRANRYFPIIEPILKANGIPDDFKYLMVIESNLNNIARSPAGAAGLWQFMPATGREFGLEVNE
NVDERYHIEKATVAACKYFKQAYAKYGDWMAVSAAYNAGQGRISSQLDQQLASHAMDLWLVEETSRYMFRLLAVKEIFKN
PQRYGFLLKKEHLYPPIPYKEITVTTPIANLSDFAKQQGITYAQLRDANPWLREQTLKNRTGKTYVLQIPTQEGMYYDPK
KTVAYNKHWVID

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=237, Percent_Identity=30.379746835443, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 38577; Mature: 38446

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRKQKMKKFTKINYILTFILVFCIGATLPILIGSSHVDEQHSAKSEVPYCVTSPTVPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCCCC
QVTFDGETIDLRRYDRRERMDREMMAFTYMHSTTMLLIKRANRYFPIIEPILKANGIPDD
EEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
FKYLMVIESNLNNIARSPAGAAGLWQFMPATGREFGLEVNENVDERYHIEKATVAACKYF
CEEEEEEECCHHHHHCCCCCHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KQAYAKYGDWMAVSAAYNAGQGRISSQLDQQLASHAMDLWLVEETSRYMFRLLAVKEIFK
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHC
NPQRYGFLLKKEHLYPPIPYKEITVTTPIANLSDFAKQQGITYAQLRDANPWLREQTLKN
CHHHHCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHC
RTGKTYVLQIPTQEGMYYDPKKTVAYNKHWVID
CCCCEEEEEECCCCCCEECCCCCEEECCCEECC
>Mature Secondary Structure 
TKRKQKMKKFTKINYILTFILVFCIGATLPILIGSSHVDEQHSAKSEVPYCVTSPTVPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCCCCC
QVTFDGETIDLRRYDRRERMDREMMAFTYMHSTTMLLIKRANRYFPIIEPILKANGIPDD
EEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
FKYLMVIESNLNNIARSPAGAAGLWQFMPATGREFGLEVNENVDERYHIEKATVAACKYF
CEEEEEEECCHHHHHCCCCCHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KQAYAKYGDWMAVSAAYNAGQGRISSQLDQQLASHAMDLWLVEETSRYMFRLLAVKEIFK
HHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHC
NPQRYGFLLKKEHLYPPIPYKEITVTTPIANLSDFAKQQGITYAQLRDANPWLREQTLKN
CHHHHCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHC
RTGKTYVLQIPTQEGMYYDPKKTVAYNKHWVID
CCCCEEEEEECCCCCCEECCCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]