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The map label for this gene is argG [H]

Identifier: 29349168

GI number: 29349168

Start: 4880574

End: 4881782

Strand: Reverse

Name: argG [H]

Synonym: BT_3760

Alternate gene names: 29349168

Gene position: 4881782-4880574 (Counterclockwise)

Preceding gene: 29349169

Following gene: 29349167

Centisome position: 77.98

GC content: 48.06

Gene sequence:

>1209_bases
ATGGAAGAAAAGAAGAAAAAAGTAGTGGTGGCATTCAGCGGCGGACTGGACACATCGTTCACCGTCATGTACCTCGCTAA
AGAAAAAGGTTACGAAGTATATGCAGCTTGTGCCAATACGGGCGGTTTCAGCGAAGAACAGCTGAAGACAAACGAAGAAA
ACGCCTACAAACTGGGAGCAGTGAAATATGTAACATTGGACGTTACTCAGGAATACTACGAAAAGAGTCTGAAATATATG
GTTTTCGGAAATGTGCTGCGTAATGGTACTTACCCGATCTCTGTCAGCTCGGAACGTATCTTCCAGGCATTGGCCATCGC
ACGTTATGCCAACGAAATCGGTGCGGATGCCATTGCCCACGGTTCTACCGGAGCAGGTAACGACCAGATTCGTTTTGACA
TGACTTTCCTTGTACTGGCTCCGAATGTAGAAATCATCACGCTGACCCGTGATATGGCATTAAGCCGTCAGGAAGAAATC
GACTATCTGAACAAACATGGTTTCAGCGCGGACTTCACCAAACTGAAATACTCTTATAACGTAGGTTTGTGGGGTACATC
CATCTGTGGTGGCGAAATCCTCGACTCCGCACAGGGATTACCGGAAACAGCCTACCTGAAACATGTGGAAAAAGAAGGCA
GCGAACAACTCCGTCTCACTTTCGAAAAGGGAGAACTGAAAGCTGTCAATGACGAAACATTCGATGATCCGATCCAAGCT
ATCCAGAAGGTAGAAGAGATCGGTGCCGCATACGGTATCGGACGTGATATGCACGTGGGCGATACGATTATCGGCATCAA
AGGCCGTGTGGGTTTCGAGGCTGCCGCTCCGATGTTGATTATCGGTGCTCACCGCTTCCTCGAAAAATACACATTGAGCA
AATGGCAGCAATACTGGAAAGATCAGGTGGCCAACTGGTACGGAATGTTCCTCCACGAAAGCCAGTATCTCGAACCGGTG
ATGCGTGACATCGAAGCCATGCTTCAGGAAAGCCAGCGTAACGTTAACGGTACGGCAATCCTCGAGCTCCGCCCGCTTTC
TTTCTCTACCGTCGGTGTAGAATCGGAAGACGACCTCGTGAAAACGAAGTTCGGCGAATATGGTGAAATGCAGAAGGGCT
GGACAGCCGAAGATGCTAAGGGCTTTATTAAGGTAACTTCTACTCCGCTACGTGTTTACTACAATAACCATAAAGACGAA
GAGATATGA

Upstream 100 bases:

>100_bases
CGTAAGTTCTGCATCTGCACAGCCATGCTGTACGATCCGACAGACCCACGGAACATAAAAAAAGAACAAGAAAGAAATAA
CATTTAATATATACAGCACT

Downstream 100 bases:

>100_bases
TTAAAGCAGGAATCATTGGTGGCGCAGGATATACAGCAGGCGAATTAATCCGTCTGCTGCTCAACCATCCGGAAACTGAA
ATCGTATTCATCAACAGCAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEEKKKKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKTNEENAYKLGAVKYVTLDVTQEYYEKSLKYM
VFGNVLRNGTYPISVSSERIFQALAIARYANEIGADAIAHGSTGAGNDQIRFDMTFLVLAPNVEIITLTRDMALSRQEEI
DYLNKHGFSADFTKLKYSYNVGLWGTSICGGEILDSAQGLPETAYLKHVEKEGSEQLRLTFEKGELKAVNDETFDDPIQA
IQKVEEIGAAYGIGRDMHVGDTIIGIKGRVGFEAAAPMLIIGAHRFLEKYTLSKWQQYWKDQVANWYGMFLHESQYLEPV
MRDIEAMLQESQRNVNGTAILELRPLSFSTVGVESEDDLVKTKFGEYGEMQKGWTAEDAKGFIKVTSTPLRVYYNNHKDE
EI

Sequences:

>Translated_402_residues
MEEKKKKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKTNEENAYKLGAVKYVTLDVTQEYYEKSLKYM
VFGNVLRNGTYPISVSSERIFQALAIARYANEIGADAIAHGSTGAGNDQIRFDMTFLVLAPNVEIITLTRDMALSRQEEI
DYLNKHGFSADFTKLKYSYNVGLWGTSICGGEILDSAQGLPETAYLKHVEKEGSEQLRLTFEKGELKAVNDETFDDPIQA
IQKVEEIGAAYGIGRDMHVGDTIIGIKGRVGFEAAAPMLIIGAHRFLEKYTLSKWQQYWKDQVANWYGMFLHESQYLEPV
MRDIEAMLQESQRNVNGTAILELRPLSFSTVGVESEDDLVKTKFGEYGEMQKGWTAEDAKGFIKVTSTPLRVYYNNHKDE
EI
>Mature_402_residues
MEEKKKKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKTNEENAYKLGAVKYVTLDVTQEYYEKSLKYM
VFGNVLRNGTYPISVSSERIFQALAIARYANEIGADAIAHGSTGAGNDQIRFDMTFLVLAPNVEIITLTRDMALSRQEEI
DYLNKHGFSADFTKLKYSYNVGLWGTSICGGEILDSAQGLPETAYLKHVEKEGSEQLRLTFEKGELKAVNDETFDDPIQA
IQKVEEIGAAYGIGRDMHVGDTIIGIKGRVGFEAAAPMLIIGAHRFLEKYTLSKWQQYWKDQVANWYGMFLHESQYLEPV
MRDIEAMLQESQRNVNGTAILELRPLSFSTVGVESEDDLVKTKFGEYGEMQKGWTAEDAKGFIKVTSTPLRVYYNNHKDE
EI

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=26.980198019802, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=26.980198019802, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1789563, Length=362, Percent_Identity=23.7569060773481, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=28.8135593220339, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358151, Length=409, Percent_Identity=26.6503667481663, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45156; Mature: 45156

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKKKKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKTNEENAYKLGA
CCCCCCEEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHCCCCCCCEEECE
VKYVTLDVTQEYYEKSLKYMVFGNVLRNGTYPISVSSERIFQALAIARYANEIGADAIAH
EEEEEEECHHHHHHHHHHEEEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCHHEEC
GSTGAGNDQIRFDMTFLVLAPNVEIITLTRDMALSRQEEIDYLNKHGFSADFTKLKYSYN
CCCCCCCCEEEEEEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
VGLWGTSICGGEILDSAQGLPETAYLKHVEKEGSEQLRLTFEKGELKAVNDETFDDPIQA
ECCEECCCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHH
IQKVEEIGAAYGIGRDMHVGDTIIGIKGRVGFEAAAPMLIIGAHRFLEKYTLSKWQQYWK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHH
DQVANWYGMFLHESQYLEPVMRDIEAMLQESQRNVNGTAILELRPLSFSTVGVESEDDLV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCHH
KTKFGEYGEMQKGWTAEDAKGFIKVTSTPLRVYYNNHKDEEI
HHCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCC
>Mature Secondary Structure
MEEKKKKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKTNEENAYKLGA
CCCCCCEEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHCCCCCCCEEECE
VKYVTLDVTQEYYEKSLKYMVFGNVLRNGTYPISVSSERIFQALAIARYANEIGADAIAH
EEEEEEECHHHHHHHHHHEEEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCHHEEC
GSTGAGNDQIRFDMTFLVLAPNVEIITLTRDMALSRQEEIDYLNKHGFSADFTKLKYSYN
CCCCCCCCEEEEEEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
VGLWGTSICGGEILDSAQGLPETAYLKHVEKEGSEQLRLTFEKGELKAVNDETFDDPIQA
ECCEECCCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHH
IQKVEEIGAAYGIGRDMHVGDTIIGIKGRVGFEAAAPMLIIGAHRFLEKYTLSKWQQYWK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHHH
DQVANWYGMFLHESQYLEPVMRDIEAMLQESQRNVNGTAILELRPLSFSTVGVESEDDLV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCHH
KTKFGEYGEMQKGWTAEDAKGFIKVTSTPLRVYYNNHKDEEI
HHCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]