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The map label for this gene is murE [H]

Identifier: 29348861

GI number: 29348861

Start: 4447131

End: 4448579

Strand: Reverse

Name: murE [H]

Synonym: BT_3452

Alternate gene names: 29348861

Gene position: 4448579-4447131 (Counterclockwise)

Preceding gene: 29348862

Following gene: 29348860

Centisome position: 71.06

GC content: 48.17

Gene sequence:

>1449_bases
ATGTTATTAAACGAGTTACTGAAAGCAATCCAGCCGGTGCAAGTGACCGGAGATTCAAGGATAGAGATCACCGGAGTCAA
CATTGACTCCCGTCTGGTAGAAGCCGGACAGCTGTTTATGGCGATGCGCGGCACGCAGACCGATGGTCACGCCTATATCC
CCGCTGCCATTCAGAAGGGAGCGACTGCCATCCTCTGCGAAGATATACCGGAGGAACCCGTAGCAGGAATCACTTATATC
CAAGTGAAAGACAGCGAAGATGCAGTAGGAAAGATTGCAACGACATTCTACGGTAATCCGACTTCACAATTCGAACTGGT
CGGTGTGACGGGAACAAATGGCAAGACAACCATCGCCACCTTATTATATAATACATTCCGCTACTTCGGATATAAAGTGG
GATTAATCTCTACTGTCTGCAACTATATCGACGATCAGCCGATTCCGACAGAACATACCACCCCCGACCCTATCACGCTC
AACCGCTTGTTGGGGCAAATGGCGGACGAAGGATGCAAGTACGTCTTTATGGAAGTCAGTTCACATTCCATCGCTCAAAA
ACGTATCAGCGGATTAAAGTTTGCGGGAGGTATCTTCACGAATCTGACGCGCGATCATCTGGATTATCACAAAACGGTAG
AGAACTATCTGAAGGCAAAGAAAAAGTTCTTTGACGATATGCCTAAAAACGCTTTCAGCCTCACCAACCTTGACGATAAG
AACGGGCTGGTGATGACACAAAATACACGTTCCAAGGTCTACACCTATTCATTAAGAAGCCTCAGCGACTTCAAAGGCCG
GGTCATAGAATCCCATTTTGAAGGAATGCTGCTCGATTTCAATAATCATGAGCTGGCCGTTCAGTTTATCGGCAAGTTCA
ATGCATCCAATCTGTTGGCGGTATTTGGAGCAGCCGTATTATTGGGCAAAAAAGAGGAAGATGTACTTCTTGCGCTCAGT
ACGCTCCATCCGGTAGCCGGACGTTTCGATGCCATCCGTTCTCCGAAAGGGGTAACGGCCATCGTTGACTATGCACATAC
TCCGGACGCGTTGGTGAATGTATTGAACGCCATTCATGGAGTACTCGAAGGCAAAGGAAAAGTGATCACCGTAGTAGGTG
CCGGCGGCAACCGCGATAAGGGGAAACGCCCCATCATGGCAAAGGAGGCAGCGAAAGCCAGTGACCGGGTGATTATCACA
TCGGACAATCCGCGTTTTGAAGAGCCACAGGAGATTATCAACGATATGCTTGCCGGACTGGATGCGGAAGATATGAAAAA
AACGCTCAGCATCGCCGACCGGAAAGAAGCGATCCGCACGGCTTGCATGCTTGCAGAAAAAGGAGACGTGATACTCGTTG
CCGGAAAAGGGCACGAAAACTATCAGGAAATCAAAGGAGTGAAACACCACTTTGACGACAAGGAAGTTTTGAAGGAAATA
TTTAATTGA

Upstream 100 bases:

>100_bases
GGAAAGTAAAAAGCCAGTCGATAGCCAACGGCAGTGTTGCAAGAAAAGGGCAGACGATTACGCTTACGTTGCATTAAGAA
TTAAATTAAGTAGGACAGAT

Downstream 100 bases:

>100_bases
CAACATATAATTATTTGCAATAGACAGCATCTTATCAAGCGTGGGGCAACAATGAGAAACTGTAAATTGCAGGCTATAAA
TTGTAAATAGAAAAGAGATG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MLLNELLKAIQPVQVTGDSRIEITGVNIDSRLVEAGQLFMAMRGTQTDGHAYIPAAIQKGATAILCEDIPEEPVAGITYI
QVKDSEDAVGKIATTFYGNPTSQFELVGVTGTNGKTTIATLLYNTFRYFGYKVGLISTVCNYIDDQPIPTEHTTPDPITL
NRLLGQMADEGCKYVFMEVSSHSIAQKRISGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDMPKNAFSLTNLDDK
NGLVMTQNTRSKVYTYSLRSLSDFKGRVIESHFEGMLLDFNNHELAVQFIGKFNASNLLAVFGAAVLLGKKEEDVLLALS
TLHPVAGRFDAIRSPKGVTAIVDYAHTPDALVNVLNAIHGVLEGKGKVITVVGAGGNRDKGKRPIMAKEAAKASDRVIIT
SDNPRFEEPQEIINDMLAGLDAEDMKKTLSIADRKEAIRTACMLAEKGDVILVAGKGHENYQEIKGVKHHFDDKEVLKEI
FN

Sequences:

>Translated_482_residues
MLLNELLKAIQPVQVTGDSRIEITGVNIDSRLVEAGQLFMAMRGTQTDGHAYIPAAIQKGATAILCEDIPEEPVAGITYI
QVKDSEDAVGKIATTFYGNPTSQFELVGVTGTNGKTTIATLLYNTFRYFGYKVGLISTVCNYIDDQPIPTEHTTPDPITL
NRLLGQMADEGCKYVFMEVSSHSIAQKRISGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDMPKNAFSLTNLDDK
NGLVMTQNTRSKVYTYSLRSLSDFKGRVIESHFEGMLLDFNNHELAVQFIGKFNASNLLAVFGAAVLLGKKEEDVLLALS
TLHPVAGRFDAIRSPKGVTAIVDYAHTPDALVNVLNAIHGVLEGKGKVITVVGAGGNRDKGKRPIMAKEAAKASDRVIIT
SDNPRFEEPQEIINDMLAGLDAEDMKKTLSIADRKEAIRTACMLAEKGDVILVAGKGHENYQEIKGVKHHFDDKEVLKEI
FN
>Mature_482_residues
MLLNELLKAIQPVQVTGDSRIEITGVNIDSRLVEAGQLFMAMRGTQTDGHAYIPAAIQKGATAILCEDIPEEPVAGITYI
QVKDSEDAVGKIATTFYGNPTSQFELVGVTGTNGKTTIATLLYNTFRYFGYKVGLISTVCNYIDDQPIPTEHTTPDPITL
NRLLGQMADEGCKYVFMEVSSHSIAQKRISGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDMPKNAFSLTNLDDK
NGLVMTQNTRSKVYTYSLRSLSDFKGRVIESHFEGMLLDFNNHELAVQFIGKFNASNLLAVFGAAVLLGKKEEDVLLALS
TLHPVAGRFDAIRSPKGVTAIVDYAHTPDALVNVLNAIHGVLEGKGKVITVVGAGGNRDKGKRPIMAKEAAKASDRVIIT
SDNPRFEEPQEIINDMLAGLDAEDMKKTLSIADRKEAIRTACMLAEKGDVILVAGKGHENYQEIKGVKHHFDDKEVLKEI
FN

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=477, Percent_Identity=37.7358490566038, Blast_Score=265, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52932; Mature: 52932

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNELLKAIQPVQVTGDSRIEITGVNIDSRLVEAGQLFMAMRGTQTDGHAYIPAAIQKG
CCHHHHHHHCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHCC
ATAILCEDIPEEPVAGITYIQVKDSEDAVGKIATTFYGNPTSQFELVGVTGTNGKTTIAT
CEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHH
LLYNTFRYFGYKVGLISTVCNYIDDQPIPTEHTTPDPITLNRLLGQMADEGCKYVFMEVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECC
SHSIAQKRISGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDMPKNAFSLTNLDDK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NGLVMTQNTRSKVYTYSLRSLSDFKGRVIESHFEGMLLDFNNHELAVQFIGKFNASNLLA
CCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHH
VFGAAVLLGKKEEDVLLALSTLHPVAGRFDAIRSPKGVTAIVDYAHTPDALVNVLNAIHG
HHHHHHHCCCCCCCCEEEEHHHCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
VLEGKGKVITVVGAGGNRDKGKRPIMAKEAAKASDRVIITSDNPRFEEPQEIINDMLAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
DAEDMKKTLSIADRKEAIRTACMLAEKGDVILVAGKGHENYQEIKGVKHHFDDKEVLKEI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
FN
CC
>Mature Secondary Structure
MLLNELLKAIQPVQVTGDSRIEITGVNIDSRLVEAGQLFMAMRGTQTDGHAYIPAAIQKG
CCHHHHHHHCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHCC
ATAILCEDIPEEPVAGITYIQVKDSEDAVGKIATTFYGNPTSQFELVGVTGTNGKTTIAT
CEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHH
LLYNTFRYFGYKVGLISTVCNYIDDQPIPTEHTTPDPITLNRLLGQMADEGCKYVFMEVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECC
SHSIAQKRISGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKKFFDDMPKNAFSLTNLDDK
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NGLVMTQNTRSKVYTYSLRSLSDFKGRVIESHFEGMLLDFNNHELAVQFIGKFNASNLLA
CCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHH
VFGAAVLLGKKEEDVLLALSTLHPVAGRFDAIRSPKGVTAIVDYAHTPDALVNVLNAIHG
HHHHHHHCCCCCCCCEEEEHHHCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
VLEGKGKVITVVGAGGNRDKGKRPIMAKEAAKASDRVIITSDNPRFEEPQEIINDMLAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
DAEDMKKTLSIADRKEAIRTACMLAEKGDVILVAGKGHENYQEIKGVKHHFDDKEVLKEI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
FN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA