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The map label for this gene is murD [H]

Identifier: 29348859

GI number: 29348859

Start: 4444262

End: 4445503

Strand: Reverse

Name: murD [H]

Synonym: BT_3450

Alternate gene names: 29348859

Gene position: 4445503-4444262 (Counterclockwise)

Preceding gene: 29348860

Following gene: 29348858

Centisome position: 71.01

GC content: 48.47

Gene sequence:

>1242_bases
ATGTCCGCTATCAAGGATAAGTATAAGGACCTTCTGGACAGCCACCATATTGCCTGGGAGGAAGGACATCATACCGAAGA
ACTCATTCTGAATGCCGATGAGGTGATTAAAAGCCCCGGTATCCCGAATGACGCCCCGCTGATCCTGAAACTGAAAGCCC
AAGGCACACCGGTTATCTCCGAAATCGAGTTTGCCGGACGCTATACCGATGCAAAAATGATCTGTATCACCGGTTCTAAT
GGAAAGACAACGACTACTTCCCTGATCTACCACATCTTTAAGAGTGCAGATCTGAATGTAGGACTAGCCGGAAATATCGG
CAAGAGCCTGGCTTTGCAAGTAGCCGAAGAGCATCATGACTATTATATCATCGAACTCAGCTCTTTCCAACTGGACAATA
TGTATAACTTCCGTGCCAATATTGCCGTACTGATGAATATCACTCCGGACCATCTGGACCGATACGACCATTGTATGCAG
AACTACATCGATGCCAAATTCCGTATTACGCAGAACCAGACAACGGACGACGCTTTCATCTTCTGGAACGATGATCCGAT
CATCAAACAGGAACTGGCAAAGCACGGTCTGAAAGCTCATCTGTATCCTTTCGCAGCCGTGAAGGAAGACGGAGCCATCG
CATACGTAGAAGATCACGAAGTGAAGATTACCGAGCCCATCGCCTTCAACATGGAACAGGAGGAATTGGCACTGACCGGA
CAGCACAATCTTTACAACTCGCTGGCTGCCGGTATCTCGGCAAACCTGGCAGGTATCACTAAAGAAAATATCCGCAAGGC
GCTCTCCGACTTCAAGGGAGTGGAACATCGCCTGGAGAAAGTAGCGCGCGTACGCGGTATCGACTTCATCAACGACTCAA
AAGCAACCAATGTCAACTCTTGCTGGTATGCCCTGCAAAGCATGACCACCAAGACAGTACTGATACTGGGCGGCAAAGAC
AAAGGCAACGACTATACGGAAATAGAAGACCTGGTACGTGAAAAGTGCTCGGCACTGGTTTATCTGGGACTGCACAACGA
AAAGCTGCACGCCTTTTTCGACCGTTTCGGACTGCCCGTAGCCGATGTGCAGACCGGAATGAAAGATGCAGTAGAAGCAG
CCTACAAACTGGCAAAGAAAGGAGAAACCGTATTGTTAAGCCCCTGCTGCGCCTCATTCGACCTCTTCAAGAGTTACGAA
GACCGCGGCGATCAGTTTAAGAAATATGTAAGAGAATTATAA

Upstream 100 bases:

>100_bases
ACTGTAAATGAAAAGAATTGTAATTTTAGGAGCCGGTGAAAGCGGCGCTGGTGCAGCCGTACTCGCCAAAGTAAAAGGAT
TTGAAACATTTGTTTCGGAT

Downstream 100 bases:

>100_bases
AATGGATTTATTAAAGAGCATATTTAAAGGCGACAAGGTAATCTGGATTATCTTCCTCTGTCTCTGCCTCATCTCGATTA
TCGAGGTGTTTTCGGCAGCA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSAIKDKYKDLLDSHHIAWEEGHHTEELILNADEVIKSPGIPNDAPLILKLKAQGTPVISEIEFAGRYTDAKMICITGSN
GKTTTTSLIYHIFKSADLNVGLAGNIGKSLALQVAEEHHDYYIIELSSFQLDNMYNFRANIAVLMNITPDHLDRYDHCMQ
NYIDAKFRITQNQTTDDAFIFWNDDPIIKQELAKHGLKAHLYPFAAVKEDGAIAYVEDHEVKITEPIAFNMEQEELALTG
QHNLYNSLAAGISANLAGITKENIRKALSDFKGVEHRLEKVARVRGIDFINDSKATNVNSCWYALQSMTTKTVLILGGKD
KGNDYTEIEDLVREKCSALVYLGLHNEKLHAFFDRFGLPVADVQTGMKDAVEAAYKLAKKGETVLLSPCCASFDLFKSYE
DRGDQFKKYVREL

Sequences:

>Translated_413_residues
MSAIKDKYKDLLDSHHIAWEEGHHTEELILNADEVIKSPGIPNDAPLILKLKAQGTPVISEIEFAGRYTDAKMICITGSN
GKTTTTSLIYHIFKSADLNVGLAGNIGKSLALQVAEEHHDYYIIELSSFQLDNMYNFRANIAVLMNITPDHLDRYDHCMQ
NYIDAKFRITQNQTTDDAFIFWNDDPIIKQELAKHGLKAHLYPFAAVKEDGAIAYVEDHEVKITEPIAFNMEQEELALTG
QHNLYNSLAAGISANLAGITKENIRKALSDFKGVEHRLEKVARVRGIDFINDSKATNVNSCWYALQSMTTKTVLILGGKD
KGNDYTEIEDLVREKCSALVYLGLHNEKLHAFFDRFGLPVADVQTGMKDAVEAAYKLAKKGETVLLSPCCASFDLFKSYE
DRGDQFKKYVREL
>Mature_412_residues
SAIKDKYKDLLDSHHIAWEEGHHTEELILNADEVIKSPGIPNDAPLILKLKAQGTPVISEIEFAGRYTDAKMICITGSNG
KTTTTSLIYHIFKSADLNVGLAGNIGKSLALQVAEEHHDYYIIELSSFQLDNMYNFRANIAVLMNITPDHLDRYDHCMQN
YIDAKFRITQNQTTDDAFIFWNDDPIIKQELAKHGLKAHLYPFAAVKEDGAIAYVEDHEVKITEPIAFNMEQEELALTGQ
HNLYNSLAAGISANLAGITKENIRKALSDFKGVEHRLEKVARVRGIDFINDSKATNVNSCWYALQSMTTKTVLILGGKDK
GNDYTEIEDLVREKCSALVYLGLHNEKLHAFFDRFGLPVADVQTGMKDAVEAAYKLAKKGETVLLSPCCASFDLFKSYED
RGDQFKKYVREL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=420, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786279, Length=245, Percent_Identity=27.3469387755102, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46388; Mature: 46257

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIKDKYKDLLDSHHIAWEEGHHTEELILNADEVIKSPGIPNDAPLILKLKAQGTPVIS
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCCHH
EIEFAGRYTDAKMICITGSNGKTTTTSLIYHIFKSADLNVGLAGNIGKSLALQVAEEHHD
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCC
YYIIELSSFQLDNMYNFRANIAVLMNITPDHLDRYDHCMQNYIDAKFRITQNQTTDDAFI
EEEEEEECCCCCCCEECEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
FWNDDPIIKQELAKHGLKAHLYPFAAVKEDGAIAYVEDHEVKITEPIAFNMEQEELALTG
EECCCCHHHHHHHHCCCEEEECEEEEECCCCCEEEEECCCEEEECCEEECCCHHHHEECC
QHNLYNSLAAGISANLAGITKENIRKALSDFKGVEHRLEKVARVRGIDFINDSKATNVNS
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHH
CWYALQSMTTKTVLILGGKDKGNDYTEIEDLVREKCSALVYLGLHNEKLHAFFDRFGLPV
HHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCH
ADVQTGMKDAVEAAYKLAKKGETVLLSPCCASFDLFKSYEDRGDQFKKYVREL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAIKDKYKDLLDSHHIAWEEGHHTEELILNADEVIKSPGIPNDAPLILKLKAQGTPVIS
CHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCCHH
EIEFAGRYTDAKMICITGSNGKTTTTSLIYHIFKSADLNVGLAGNIGKSLALQVAEEHHD
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCC
YYIIELSSFQLDNMYNFRANIAVLMNITPDHLDRYDHCMQNYIDAKFRITQNQTTDDAFI
EEEEEEECCCCCCCEECEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
FWNDDPIIKQELAKHGLKAHLYPFAAVKEDGAIAYVEDHEVKITEPIAFNMEQEELALTG
EECCCCHHHHHHHHCCCEEEECEEEEECCCCCEEEEECCCEEEECCEEECCCHHHHEECC
QHNLYNSLAAGISANLAGITKENIRKALSDFKGVEHRLEKVARVRGIDFINDSKATNVNS
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHH
CWYALQSMTTKTVLILGGKDKGNDYTEIEDLVREKCSALVYLGLHNEKLHAFFDRFGLPV
HHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCH
ADVQTGMKDAVEAAYKLAKKGETVLLSPCCASFDLFKSYEDRGDQFKKYVREL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA