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The map label for this gene is gyrB [H]

Identifier: 29348838

GI number: 29348838

Start: 4418315

End: 4420276

Strand: Reverse

Name: gyrB [H]

Synonym: BT_3429

Alternate gene names: 29348838

Gene position: 4420276-4418315 (Counterclockwise)

Preceding gene: 29348839

Following gene: 29348837

Centisome position: 70.61

GC content: 47.3

Gene sequence:

>1962_bases
ATGAGCGAAGAACAAATCACTCCCAATAACGGGTCTTATTCTGCGGATAGCATCCAGGTATTGGAAGGTCTTGAAGCAGT
GAGAAAACGCCCTGCGATGTACATCGGTGACATCAGCATAAAGGGACTTCACCACTTGGTATACGAAATCGTTGATAACT
CCATTGACGAGGCACTGGCCGGGTATTGCGACCACATCGAAGTGACAATCAACGAAGATAATTCAATCACCGTACAGGAC
AACGGACGTGGTATTCCGGTCGACTATCATGAAAAAGAAAAGAAGTCGGCACTGGAAGTAGCGATGACCGTTCTGCACGC
CGGAGGTAAGTTCGACAAAGGGTCGTATAAAGTTTCCGGAGGTTTGCACGGTGTAGGTATGTCTTGCGTAAATGCACTGT
CAACACACATGACTACACAGGTATTCCGCAATGGTAAAATCTATCAGCAGGAATATGAAATTGGTAAGCCGCTTTATTCT
GTGAAAGAAGTGGGTGTATCGGATATTACAGGAACCCGCCAACAATTCTGGCCGGATGATACAATCTTTACCGAAACGGT
GTATGATTATAAAATACTGGCTTCACGTCTGCGTGAGCTGGCATACCTGAATGCAGGTCTCCGCATTTCACTGACCGACC
GTCGGGTAGTGAATGAAGAAGACGGTAGCTTCAAGAGTGAAGTATTCTATTCCGAAGAAGGGCTGAGAGAGTTTGTACGC
TTCATCGAGTCTTCACGTGAACACTTGATCAACGACGTTATCTATCTGAACTCTGAGAAGCAGGGAATACCTATTGAAAT
AGCCATTATGTACAATACAGGATTCTCCGAAAATGTACATTCCTATGTCAATAATATCAATACAATCGAAGGAGGTACGC
ATCTTGCCGGTTTCCGCCGTGCACTGACCCGTACGCTGAAGAAATACGCCGAAGACAGCAAGATGCTGGAAAAGGTGAAA
GTTGAAATATCGGGAGACGACTTCCGCGAAGGTCTGACTGCCGTTATTTCCGTTAAAGTTGCCGAACCACAGTTCGAAGG
ACAGACCAAAACAAAGTTGGGTAACAACGAAGTAATGGGTGCCGTAGACCAGGCTGTCGGTGAAGTTTTGGCTTACTACC
TGGAAGAACATCCAAAGGAGGCAAAAACGATTGTAGACAAGGTAATCCTTGCTGCCACAGCACGCCACGCAGCAAGAAAA
GCCCGTGAAATGGTACAACGCAAGTCTCCGATGTCCGGAGGCGGTCTGCCGGGTAAACTGGCCGACTGTTCGGATAAAGA
CCCCTCAAAATGCGAATTGTTCCTCGTCGAGGGAGACTCGGCAGGTGGTACTGCCAAGCAAGGACGTAACCGTATGTTCC
AGGCCATCCTTCCGCTACGCGGTAAGATTCTGAACGTCGAAAAAGCGATGTATCACAAAGCACTGGAAAGCGATGAAATC
CGCAATATATATACGGCACTGGGTGTCACTATCGGAACAGAAGACGACAGCAAAGAGGCTAACATCGAAAAGCTGCGCTA
TCACAAGATTATCATCATGACCGATGCCGACGTCGATGGTTCTCACATCGACACACTGATCATGACATTCTTCTTCCGTT
ATATGCCGCAGATTATTCAGAACGGTTATCTATACATCGCTACTCCCCCGCTCTACCTCTGCAAAAAGGGTAAAGTAGAA
GAGTATTGCTGGACAGACGCGCAACGCCAGAAGTTTATCGATACCTATGGCGGCGGTCTGGAAAATGCGGTTCATACACA
GCGCTACAAAGGTTTGGGTGAAATGAACGCCCAGCAGTTGTGGGAAACAACAATGGACCCCGACAACCGTATGCTGAAGC
AAGTAAACATCGATAATGCCGCCGAAGCCGATTACATCTTCTCCATGCTAATGGGTGAAGACGTAGGTCCGCGCCGCGAG
TTTATCGAAGAGAATGCAACATATGCGAATATCGATACATAA

Upstream 100 bases:

>100_bases
ACTAATTCCGCTTTTATTCTGCATTTTTCGCCTCCGAAACTTCGCCAACTCGACAATTTTCACTATATTTGCTCTGTTAT
TATAACTTAGAAAGGCAATT

Downstream 100 bases:

>100_bases
TTTTAATATTACGTTTTTATAAACCAACCTCACATCTTACAACGAAGAAGCGTCGGCAAGCGAAGGAAAATCCTCCGTGT
CGGCGCTTTTCTATGTCTTA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MSEEQITPNNGSYSADSIQVLEGLEAVRKRPAMYIGDISIKGLHHLVYEIVDNSIDEALAGYCDHIEVTINEDNSITVQD
NGRGIPVDYHEKEKKSALEVAMTVLHAGGKFDKGSYKVSGGLHGVGMSCVNALSTHMTTQVFRNGKIYQQEYEIGKPLYS
VKEVGVSDITGTRQQFWPDDTIFTETVYDYKILASRLRELAYLNAGLRISLTDRRVVNEEDGSFKSEVFYSEEGLREFVR
FIESSREHLINDVIYLNSEKQGIPIEIAIMYNTGFSENVHSYVNNINTIEGGTHLAGFRRALTRTLKKYAEDSKMLEKVK
VEISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNNEVMGAVDQAVGEVLAYYLEEHPKEAKTIVDKVILAATARHAARK
AREMVQRKSPMSGGGLPGKLADCSDKDPSKCELFLVEGDSAGGTAKQGRNRMFQAILPLRGKILNVEKAMYHKALESDEI
RNIYTALGVTIGTEDDSKEANIEKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMPQIIQNGYLYIATPPLYLCKKGKVE
EYCWTDAQRQKFIDTYGGGLENAVHTQRYKGLGEMNAQQLWETTMDPDNRMLKQVNIDNAAEADYIFSMLMGEDVGPRRE
FIEENATYANIDT

Sequences:

>Translated_653_residues
MSEEQITPNNGSYSADSIQVLEGLEAVRKRPAMYIGDISIKGLHHLVYEIVDNSIDEALAGYCDHIEVTINEDNSITVQD
NGRGIPVDYHEKEKKSALEVAMTVLHAGGKFDKGSYKVSGGLHGVGMSCVNALSTHMTTQVFRNGKIYQQEYEIGKPLYS
VKEVGVSDITGTRQQFWPDDTIFTETVYDYKILASRLRELAYLNAGLRISLTDRRVVNEEDGSFKSEVFYSEEGLREFVR
FIESSREHLINDVIYLNSEKQGIPIEIAIMYNTGFSENVHSYVNNINTIEGGTHLAGFRRALTRTLKKYAEDSKMLEKVK
VEISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNNEVMGAVDQAVGEVLAYYLEEHPKEAKTIVDKVILAATARHAARK
AREMVQRKSPMSGGGLPGKLADCSDKDPSKCELFLVEGDSAGGTAKQGRNRMFQAILPLRGKILNVEKAMYHKALESDEI
RNIYTALGVTIGTEDDSKEANIEKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMPQIIQNGYLYIATPPLYLCKKGKVE
EYCWTDAQRQKFIDTYGGGLENAVHTQRYKGLGEMNAQQLWETTMDPDNRMLKQVNIDNAAEADYIFSMLMGEDVGPRRE
FIEENATYANIDT
>Mature_652_residues
SEEQITPNNGSYSADSIQVLEGLEAVRKRPAMYIGDISIKGLHHLVYEIVDNSIDEALAGYCDHIEVTINEDNSITVQDN
GRGIPVDYHEKEKKSALEVAMTVLHAGGKFDKGSYKVSGGLHGVGMSCVNALSTHMTTQVFRNGKIYQQEYEIGKPLYSV
KEVGVSDITGTRQQFWPDDTIFTETVYDYKILASRLRELAYLNAGLRISLTDRRVVNEEDGSFKSEVFYSEEGLREFVRF
IESSREHLINDVIYLNSEKQGIPIEIAIMYNTGFSENVHSYVNNINTIEGGTHLAGFRRALTRTLKKYAEDSKMLEKVKV
EISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNNEVMGAVDQAVGEVLAYYLEEHPKEAKTIVDKVILAATARHAARKA
REMVQRKSPMSGGGLPGKLADCSDKDPSKCELFLVEGDSAGGTAKQGRNRMFQAILPLRGKILNVEKAMYHKALESDEIR
NIYTALGVTIGTEDDSKEANIEKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMPQIIQNGYLYIATPPLYLCKKGKVEE
YCWTDAQRQKFIDTYGGGLENAVHTQRYKGLGEMNAQQLWETTMDPDNRMLKQVNIDNAAEADYIFSMLMGEDVGPRREF
IEENATYANIDT

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=633, Percent_Identity=27.3301737756714, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19913406, Length=620, Percent_Identity=26.1290322580645, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=55.6350626118068, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789408, Length=648, Percent_Identity=36.8827160493827, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535065, Length=650, Percent_Identity=24.9230769230769, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=570, Percent_Identity=25.0877192982456, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=158, Percent_Identity=33.5443037974684, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=687, Percent_Identity=25.9097525473071, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17136538, Length=640, Percent_Identity=25.78125, Blast_Score=139, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73249; Mature: 73118

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEQITPNNGSYSADSIQVLEGLEAVRKRPAMYIGDISIKGLHHLVYEIVDNSIDEALA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECEEHHHHHHHHHHHHHCHHHHHHH
GYCDHIEVTINEDNSITVQDNGRGIPVDYHEKEKKSALEVAMTVLHAGGKFDKGSYKVSG
CCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGMSCVNALSTHMTTQVFRNGKIYQQEYEIGKPLYSVKEVGVSDITGTRQQFWPDD
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCC
TIFTETVYDYKILASRLRELAYLNAGLRISLTDRRVVNEEDGSFKSEVFYSEEGLREFVR
CEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCHHHHHEECHHHHHHHHH
FIESSREHLINDVIYLNSEKQGIPIEIAIMYNTGFSENVHSYVNNINTIEGGTHLAGFRR
HHHHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHH
ALTRTLKKYAEDSKMLEKVKVEISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNNEVMG
HHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCEEEEEEEEECCCCCCCCHHCCCCCHHHH
AVDQAVGEVLAYYLEEHPKEAKTIVDKVILAATARHAARKAREMVQRKSPMSGGGLPGKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ADCSDKDPSKCELFLVEGDSAGGTAKQGRNRMFQAILPLRGKILNVEKAMYHKALESDEI
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHH
RNIYTALGVTIGTEDDSKEANIEKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMPQIIQ
HHHHHHHCEEECCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
NGYLYIATPPLYLCKKGKVEEYCWTDAQRQKFIDTYGGGLENAVHTQRYKGLGEMNAQQL
CCEEEEECCCHHCCCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
WETTMDPDNRMLKQVNIDNAAEADYIFSMLMGEDVGPRREFIEENATYANIDT
HHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
SEEQITPNNGSYSADSIQVLEGLEAVRKRPAMYIGDISIKGLHHLVYEIVDNSIDEALA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECEEHHHHHHHHHHHHHCHHHHHHH
GYCDHIEVTINEDNSITVQDNGRGIPVDYHEKEKKSALEVAMTVLHAGGKFDKGSYKVSG
CCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGMSCVNALSTHMTTQVFRNGKIYQQEYEIGKPLYSVKEVGVSDITGTRQQFWPDD
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCC
TIFTETVYDYKILASRLRELAYLNAGLRISLTDRRVVNEEDGSFKSEVFYSEEGLREFVR
CEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCHHHHHEECHHHHHHHHH
FIESSREHLINDVIYLNSEKQGIPIEIAIMYNTGFSENVHSYVNNINTIEGGTHLAGFRR
HHHHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHH
ALTRTLKKYAEDSKMLEKVKVEISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNNEVMG
HHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCEEEEEEEEECCCCCCCCHHCCCCCHHHH
AVDQAVGEVLAYYLEEHPKEAKTIVDKVILAATARHAARKAREMVQRKSPMSGGGLPGKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ADCSDKDPSKCELFLVEGDSAGGTAKQGRNRMFQAILPLRGKILNVEKAMYHKALESDEI
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHH
RNIYTALGVTIGTEDDSKEANIEKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMPQIIQ
HHHHHHHCEEECCCCCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
NGYLYIATPPLYLCKKGKVEEYCWTDAQRQKFIDTYGGGLENAVHTQRYKGLGEMNAQQL
CCEEEEECCCHHCCCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHH
WETTMDPDNRMLKQVNIDNAAEADYIFSMLMGEDVGPRREFIEENATYANIDT
HHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]