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The map label for this gene is pdxB

Identifier: 29348770

GI number: 29348770

Start: 4335140

End: 4336186

Strand: Reverse

Name: pdxB

Synonym: BT_3361

Alternate gene names: 29348770

Gene position: 4336186-4335140 (Counterclockwise)

Preceding gene: 29348778

Following gene: 29348768

Centisome position: 69.26

GC content: 46.7

Gene sequence:

>1047_bases
ATGAAAATTATTATAGATGATAAGATACCTTATATAAAAGAGGCAGCAGAGAAAATAGCCGACGAGGCAATCTACGCACC
GGGAAAGGATTTTACACGGGAACTGGTACAGGATGCGGACGCACTCATCATCCGGACCCGCACTCACTGCAACCGCGAAT
TGCTGGAAGGCAGCAAAGTAAAATTTATCGCCACAGCAACCATCGGATTTGACCATATCGACACCGAATACTGCAAGCAG
GCGGGTATCGAGTGGGCAAATGCCCCGGGATGCAATTCCGCCTCGGTGGCTCAGTATATACAATCCTCTCTTCTGATCTG
GAAATCTCTTCGGAATAAAAAGCCGGACGAACTGACGATCGGCATTATCGGTGTAGGTAATGTAGGAAGCAAGGTGGCCA
AAGTCGCACAGGATTTCGGTATGCGTGTACTACTGAACGACTTGCCAAGAGAAGAGAAAGAAGGAAACATTACCTTCACC
TCACTAGAGAAAATAGCCGAAGAATGTGATATCATCACCTTTCACGTACCTTTATATAAGGAAGGGAAATATAAAACGTA
CCATCTGGCAGACGGGAATTTCTTCCGGTCTCTCCAACGGAAGCCTGTTGTCATCAACACTTCAAGAGGAGAAGTGATCG
AAACAAACGCCCTGCTGGAAGCCATCAACAACGGTATAATCTCGGACGCAGTAATCGATGTATGGGAACATGAACCGGAA
ATCAACCGTGAATTATTAGAAAAGGTACTGATAGGCACCCCGCATATCGCCGGATACTCTGCCGACGGAAAAGCAAATGC
CACCCGAATGTCGCTGGACTCCATCTGCCGCTTCTTCCACCTAAGCGCAACTTATGAAATCACCCCGCCCGCCCCATCTT
CGCCTCTTATCGAAGCAAAAGACCGCGAAGAAGCTCTGCTGAAAATGTACAATCCCATCGAAGACAGCAACCGATTAAAA
TCCCATCCGGAACTATTCGAAACCTTACGAGGAGATTATCCGTTGAGGAGAGAAGAGAAGGCGTATAATATAATAGGTAT
TAAGTAA

Upstream 100 bases:

>100_bases
AGATGTATAATAGGACAAAGCTATGTAATATTTTTCAGACAAGCAACCGTTTCTTACTTTTTTATTTCTTACCTTTGTCT
GAGCAAAAAAGGAATTGTAT

Downstream 100 bases:

>100_bases
TAAGTATTAGGTATTAACCGTAGTTGCTTAAACTTCCATTCGGCAAAAGTCCGCATGGTTAATACTTAATACCTAATACT
TAATACTTATTGTTTCTTCT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKIIIDDKIPYIKEAAEKIADEAIYAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQ
AGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFT
SLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPE
INRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFHLSATYEITPPAPSSPLIEAKDREEALLKMYNPIEDSNRLK
SHPELFETLRGDYPLRREEKAYNIIGIK

Sequences:

>Translated_348_residues
MKIIIDDKIPYIKEAAEKIADEAIYAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQ
AGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFT
SLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPE
INRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFHLSATYEITPPAPSSPLIEAKDREEALLKMYNPIEDSNRLK
SHPELFETLRGDYPLRREEKAYNIIGIK
>Mature_348_residues
MKIIIDDKIPYIKEAAEKIADEAIYAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQ
AGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFT
SLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPE
INRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFHLSATYEITPPAPSSPLIEAKDREEALLKMYNPIEDSNRLK
SHPELFETLRGDYPLRREEKAYNIIGIK

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=253, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI6912396, Length=296, Percent_Identity=27.7027027027027, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788660, Length=356, Percent_Identity=37.3595505617978, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1789279, Length=266, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787645, Length=233, Percent_Identity=26.1802575107296, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082289, Length=264, Percent_Identity=25, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=30.8270676691729, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=277, Percent_Identity=25.9927797833935, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=267, Percent_Identity=27.3408239700375, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=267, Percent_Identity=26.9662921348315, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921140, Length=287, Percent_Identity=25.4355400696864, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28574286, Length=288, Percent_Identity=25.6944444444444, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585516, Length=252, Percent_Identity=24.6031746031746, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571528, Length=273, Percent_Identity=24.1758241758242, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_BACTN (Q8A2E4)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812273.1
- ProteinModelPortal:   Q8A2E4
- SMR:   Q8A2E4
- GeneID:   1072463
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3361
- NMPDR:   fig|226186.1.peg.3360
- HOGENOM:   HBG289972
- OMA:   IEWANAP
- PhylomeDB:   Q8A2E4
- ProtClustDB:   PRK00257
- BioCyc:   BTHE226186:BT_3361-MONOMER
- BRENDA:   1.1.1.290
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 39077; Mature: 39077

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 209-209 ACT_SITE 238-238 ACT_SITE 255-255 BINDING 46-46 BINDING 67-67 BINDING 147-147 BINDING 233-233 BINDING 258-258 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIDDKIPYIKEAAEKIADEAIYAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKV
CEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECHHCCHHHHCCCCE
KFIATATIGFDHIDTEYCKQAGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTI
EEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
GIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFTSLEKIAEECDIITFHVPLYK
EEEEECCCHHHHHHHHHHHCCEEHHHHCCCCCCCCCEEHHHHHHHHHHCCEEEEEECEEE
EGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPE
CCCEEEEEECCCHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHCCCHHHHHHHHHCCCCC
INRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFHLSATYEITPPAPSSPLIEAK
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCC
DREEALLKMYNPIEDSNRLKSHPELFETLRGDYPLRREEKAYNIIGIK
CHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MKIIIDDKIPYIKEAAEKIADEAIYAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKV
CEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECHHCCHHHHCCCCE
KFIATATIGFDHIDTEYCKQAGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTI
EEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
GIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFTSLEKIAEECDIITFHVPLYK
EEEEECCCHHHHHHHHHHHCCEEHHHHCCCCCCCCCEEHHHHHHHHHHCCEEEEEECEEE
EGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPE
CCCEEEEEECCCHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHCCCHHHHHHHHHCCCCC
INRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFHLSATYEITPPAPSSPLIEAK
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCC
DREEALLKMYNPIEDSNRLKSHPELFETLRGDYPLRREEKAYNIIGIK
CHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928