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The map label for this gene is dusB [H]

Identifier: 29348735

GI number: 29348735

Start: 4274459

End: 4275403

Strand: Reverse

Name: dusB [H]

Synonym: BT_3326

Alternate gene names: 29348735

Gene position: 4275403-4274459 (Counterclockwise)

Preceding gene: 29348736

Following gene: 29348734

Centisome position: 68.29

GC content: 48.25

Gene sequence:

>945_bases
ATGCAAGATACACTACCCATCCATTTTGCTCCGCTGCAAGGTTACACCGAAGCAATTTACCGCAATGCACATGACGCCTT
CTTCGGCGGTGTAGACACCTATTATACTCCGTTCGTACGATTGGAAAAAGGGAACTTCCGGCGCAGAGATGTCCGGGGAA
TCGAACCTGAGAACAATGGGGTGCCCCACCTGATTCCTCAGTTGATAGCCTCATCGGCAGAGAAAGCAGAAGTGATTTTA
TCCCTTTTCATCGAAAAAGGATATCAGGAAGTGGATATAAACCTAGGCTGTCCGTTCCCGCTTCTGGCGAAACGTCATAA
CGGCTCCGGCATACTTCCTTATCCCGAAGAGGTGAAAGCATTGCTCAGCATTGTCACCCGATATCCGCAGATCAGCTTCT
CCGTCAAAATGAGACTGGGATGGGAACAGCCGGATGAATGCCTGGCACTCGCTCCCATACTGAATGACCTGCCTCTGCGC
CAGATCACCATGCACCCCCGTCTGGGCAAACAAGGTTACAAAGGAGAAGTTGACTTGCAAGGATTCTCAGCTTTCCGGGA
GGTCTGTCAGCTTCCGCTTGTTTACAATGGAGACATTCATAATTTGGAAGATATTCAACGCATCAGCACACAATTCCCCT
CACTGGCGGGAATCATGATAGGACGCGGACTACTGGCAAATCCGGCTTTAGCACTGGAATACAAAGAAAACCGTACGCTC
GCGCCCGATGAAATGAGGGATAGACTGAAATCCATGCACAAAAGCGTGTACAACAATTACGACGTACTATTGGAAGGAGG
AGAGGGACAGCTACTGACTAAAATGAAGACCTTTTGGGAATACCTGGCGCCCCAAACCGACAGAAAAGTACTGAAAGCGA
TTCATAAAAGTACGAATATCAGCAAATATTATCAGGCTGTCAGTGCCTTTTTTAATCAACGGTAG

Upstream 100 bases:

>100_bases
TGACCAGCCTAAAAGCCCGAAACAGAAGTATCGGCTGACAGAAAAAGGAAAAGCTTTATTAGAGAAAAGATAACGAATAA
CGAATAGCAAATTAAAAGAT

Downstream 100 bases:

>100_bases
ATTGAGAATCAGTTATTTTATGTAATTTTGCGCCCGTTTATTAACAAGCACATTATGAAAAATACATTCTATTATCTCTC
TGCTTGATGACACTATCAAG

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MQDTLPIHFAPLQGYTEAIYRNAHDAFFGGVDTYYTPFVRLEKGNFRRRDVRGIEPENNGVPHLIPQLIASSAEKAEVIL
SLFIEKGYQEVDINLGCPFPLLAKRHNGSGILPYPEEVKALLSIVTRYPQISFSVKMRLGWEQPDECLALAPILNDLPLR
QITMHPRLGKQGYKGEVDLQGFSAFREVCQLPLVYNGDIHNLEDIQRISTQFPSLAGIMIGRGLLANPALALEYKENRTL
APDEMRDRLKSMHKSVYNNYDVLLEGGEGQLLTKMKTFWEYLAPQTDRKVLKAIHKSTNISKYYQAVSAFFNQR

Sequences:

>Translated_314_residues
MQDTLPIHFAPLQGYTEAIYRNAHDAFFGGVDTYYTPFVRLEKGNFRRRDVRGIEPENNGVPHLIPQLIASSAEKAEVIL
SLFIEKGYQEVDINLGCPFPLLAKRHNGSGILPYPEEVKALLSIVTRYPQISFSVKMRLGWEQPDECLALAPILNDLPLR
QITMHPRLGKQGYKGEVDLQGFSAFREVCQLPLVYNGDIHNLEDIQRISTQFPSLAGIMIGRGLLANPALALEYKENRTL
APDEMRDRLKSMHKSVYNNYDVLLEGGEGQLLTKMKTFWEYLAPQTDRKVLKAIHKSTNISKYYQAVSAFFNQR
>Mature_314_residues
MQDTLPIHFAPLQGYTEAIYRNAHDAFFGGVDTYYTPFVRLEKGNFRRRDVRGIEPENNGVPHLIPQLIASSAEKAEVIL
SLFIEKGYQEVDINLGCPFPLLAKRHNGSGILPYPEEVKALLSIVTRYPQISFSVKMRLGWEQPDECLALAPILNDLPLR
QITMHPRLGKQGYKGEVDLQGFSAFREVCQLPLVYNGDIHNLEDIQRISTQFPSLAGIMIGRGLLANPALALEYKENRTL
APDEMRDRLKSMHKSVYNNYDVLLEGGEGQLLTKMKTFWEYLAPQTDRKVLKAIHKSTNISKYYQAVSAFFNQR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI239788462, Length=208, Percent_Identity=29.3269230769231, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=25.78125, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789660, Length=163, Percent_Identity=26.9938650306748, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=35.8620689655172, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585320, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35701; Mature: 35701

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTLPIHFAPLQGYTEAIYRNAHDAFFGGVDTYYTPFVRLEKGNFRRRDVRGIEPENNG
CCCCCCEEEECCCHHHHHHHHCCHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCC
VPHLIPQLIASSAEKAEVILSLFIEKGYQEVDINLGCPFPLLAKRHNGSGILPYPEEVKA
CCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHH
LLSIVTRYPQISFSVKMRLGWEQPDECLALAPILNDLPLRQITMHPRLGKQGYKGEVDLQ
HHHHHHHCCCCEEEEEEEECCCCCHHHEEHHHHHCCCCCHHEEECCCCCCCCCCCCCCCH
GFSAFREVCQLPLVYNGDIHNLEDIQRISTQFPSLAGIMIGRGLLANPALALEYKENRTL
HHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHCCCEEEEEECCCCCC
APDEMRDRLKSMHKSVYNNYDVLLEGGEGQLLTKMKTFWEYLAPQTDRKVLKAIHKSTNI
CCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
SKYYQAVSAFFNQR
HHHHHHHHHHHCCC
>Mature Secondary Structure
MQDTLPIHFAPLQGYTEAIYRNAHDAFFGGVDTYYTPFVRLEKGNFRRRDVRGIEPENNG
CCCCCCEEEECCCHHHHHHHHCCHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCC
VPHLIPQLIASSAEKAEVILSLFIEKGYQEVDINLGCPFPLLAKRHNGSGILPYPEEVKA
CCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHH
LLSIVTRYPQISFSVKMRLGWEQPDECLALAPILNDLPLRQITMHPRLGKQGYKGEVDLQ
HHHHHHHCCCCEEEEEEEECCCCCHHHEEHHHHHCCCCCHHEEECCCCCCCCCCCCCCCH
GFSAFREVCQLPLVYNGDIHNLEDIQRISTQFPSLAGIMIGRGLLANPALALEYKENRTL
HHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHCCCEEEEEECCCCCC
APDEMRDRLKSMHKSVYNNYDVLLEGGEGQLLTKMKTFWEYLAPQTDRKVLKAIHKSTNI
CCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
SKYYQAVSAFFNQR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]