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The map label for this gene is nodB [H]

Identifier: 29348655

GI number: 29348655

Start: 4143633

End: 4144247

Strand: Reverse

Name: nodB [H]

Synonym: BT_3246

Alternate gene names: 29348655

Gene position: 4144247-4143633 (Counterclockwise)

Preceding gene: 29348656

Following gene: 29348654

Centisome position: 66.2

GC content: 50.73

Gene sequence:

>615_bases
ATGTTTATAGAACAACCACCTTGGTTCTTCCGAGCGCTGTATCCACAAGCCATCTTCCGGATGGACCCAAACGAACGGGC
AGTCTACCTGACTTTTGACGACGGCCCTATCCCGGAAGTTACCCCTTGGGTACTGGAGCTACTGAAGAAACACGATATTA
AAGCTACCTTCTTCATGGTAGGCGACAATATCCGCAAACACCCGGACGAATACCGGATGGTGGTAGAACACGGACACCGC
ATCGGCAACCATACTTTCAACCACATCCGCGGCTTTGAATATTCCAATCCGGATTATCTGGCCAACACCAGACGGGTAGA
CGAAATTATCCACTCGGACTTGTTCCGCCCGCCACATGGGCACATGGGATTCCGGCAATATTACACCCTTCGCCACCATT
ACCGTATCATCATGTGGGATCTCGTAACCCGCGATTACAGTAAGCGAATGCGCCCGGAACAGGTACTGAACAACGTAAAA
CGCTACGTACGCAACGGTTCCATCATCACCTTCCACGACTCTCTGAAATCGTGGAACAACGGGAATCTGCAATATGCTCT
CCCCCGTGCCATCGAGTTTCTGAAAGCGGAAGGCTATGTGTTCAAGGTATTCTAG

Upstream 100 bases:

>100_bases
ACTAAGTAATAAGTATTAAGTAATAAGTAATAAGCTGCGCCATAAGCCGCACGGTTATTACTTATTACTTATTACCTGTT
ACTTATTAATAAAGACTCTT

Downstream 100 bases:

>100_bases
CTGCCGGCAAAGAAACATTTTACCTGCCGACAGCAAGTTTTCTAATTACCGATAAGGTTTTCATCCATATCCATTATGAA
AAAGCTCCTCTCCTCAGACC

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MFIEQPPWFFRALYPQAIFRMDPNERAVYLTFDDGPIPEVTPWVLELLKKHDIKATFFMVGDNIRKHPDEYRMVVEHGHR
IGNHTFNHIRGFEYSNPDYLANTRRVDEIIHSDLFRPPHGHMGFRQYYTLRHHYRIIMWDLVTRDYSKRMRPEQVLNNVK
RYVRNGSIITFHDSLKSWNNGNLQYALPRAIEFLKAEGYVFKVF

Sequences:

>Translated_204_residues
MFIEQPPWFFRALYPQAIFRMDPNERAVYLTFDDGPIPEVTPWVLELLKKHDIKATFFMVGDNIRKHPDEYRMVVEHGHR
IGNHTFNHIRGFEYSNPDYLANTRRVDEIIHSDLFRPPHGHMGFRQYYTLRHHYRIIMWDLVTRDYSKRMRPEQVLNNVK
RYVRNGSIITFHDSLKSWNNGNLQYALPRAIEFLKAEGYVFKVF
>Mature_204_residues
MFIEQPPWFFRALYPQAIFRMDPNERAVYLTFDDGPIPEVTPWVLELLKKHDIKATFFMVGDNIRKHPDEYRMVVEHGHR
IGNHTFNHIRGFEYSNPDYLANTRRVDEIIHSDLFRPPHGHMGFRQYYTLRHHYRIIMWDLVTRDYSKRMRPEQVLNNVK
RYVRNGSIITFHDSLKSWNNGNLQYALPRAIEFLKAEGYVFKVF

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323338, Length=123, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 24616; Mature: 24616

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIEQPPWFFRALYPQAIFRMDPNERAVYLTFDDGPIPEVTPWVLELLKKHDIKATFFMV
CCCCCCCHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEE
GDNIRKHPDEYRMVVEHGHRIGNHTFNHIRGFEYSNPDYLANTRRVDEIIHSDLFRPPHG
CCCHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
HMGFRQYYTLRHHYRIIMWDLVTRDYSKRMRPEQVLNNVKRYVRNGSIITFHDSLKSWNN
CCCHHHHHHHHHHHEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GNLQYALPRAIEFLKAEGYVFKVF
CCEEEEHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MFIEQPPWFFRALYPQAIFRMDPNERAVYLTFDDGPIPEVTPWVLELLKKHDIKATFFMV
CCCCCCCHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEE
GDNIRKHPDEYRMVVEHGHRIGNHTFNHIRGFEYSNPDYLANTRRVDEIIHSDLFRPPHG
CCCHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
HMGFRQYYTLRHHYRIIMWDLVTRDYSKRMRPEQVLNNVKRYVRNGSIITFHDSLKSWNN
CCCHHHHHHHHHHHEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GNLQYALPRAIEFLKAEGYVFKVF
CCEEEEHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2615763 [H]