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The map label for this gene is tuf [H]

Identifier: 29348149

GI number: 29348149

Start: 3393922

End: 3395106

Strand: Reverse

Name: tuf [H]

Synonym: BT_2740

Alternate gene names: 29348149

Gene position: 3395106-3393922 (Counterclockwise)

Preceding gene: 29348150

Following gene: 29348148

Centisome position: 54.23

GC content: 44.89

Gene sequence:

>1185_bases
ATGGCTAAAGAGAAATTTGAACGTACCAAACCGCACGTAAACATTGGTACAATCGGTCACGTAGACCACGGTAAGACAAC
GTTGACAGCTGCTATCACTACTGTGTTGGCAAAAAAAGGTCTTTCTGAGTTGCGTTCTTTCGATTCTATCGACAACGCTC
CTGAAGAAAAAGAAAGAGGTATTACTATCAATACTTCACACGTTGAGTACGAAACAGCTAACCGTCACTACGCACACGTT
GACTGTCCGGGACACGCCGACTACGTAAAGAACATGGTAACTGGTGCTGCTCAGATGGACGGTGCTATCATCGTTTGTGC
TGCAACTGATGGTCCGATGCCTCAGACTCGCGAACATATCCTGTTGGCTCGTCAGGTAAACGTACCTCGTCTGGTTGTAT
TCTTGAACAAGTGCGATATGGTAGACGACGAAGAAATGTTGGAACTCGTTGAAATGGAAATGAGAGAACTTCTTTCATTC
TATGATTTCGATGGTGACAATACTCCTATCATCCAGGGTTCTGCTCTTGGCGCATTGAACGGTGTTGAAAAATGGGAAGA
CAAAGTTATGGAACTGATGGATGCAGTTGATAACTGGATTCCACTTCCTCCGCGCGATGTTGATAAACCATTCTTGATGC
CGGTTGAAGACGTGTTCTCTATCACAGGTCGTGGTACTGTAGCAACAGGTCGTATCGAATCTGGTATCATCCACGTAGGT
GATGAAGTTGAAATCCTTGGTTTAGGTGAAGATAAGAAATCAGTTGTAACTGGTGTTGAAATGTTCCGTAAACTGTTGGA
TCAGGGTGAAGCTGGTGACAACGTAGGTTTGTTGCTCCGTGGTGTTGACAAGAACGAAATCAAACGTGGTATGGTTCTTT
GTAAACCGGGTCAGATTAAACCGCACTCTCGCTTCAAAGCTGAGGTTTATATCCTGAAGAAAGAAGAAGGTGGTCGTCAC
ACTCCGTTCCACAACAAATACCGTCCTCAGTTCTACTTGCGTACTATGGACTGTACAGGTGAAATCACTTTGCCGGAAGG
AACAGAAATGGTAATGCCGGGTGATAACGTAACTATCACTGTAGAGTTGATCTACCCAGTAGCATTGAACCCAGGTCTTC
GTTTCGCTATCCGCGAAGGTGGACGTACAGTAGGTGCTGGTCAGATTACTGAAATTATCGACTAA

Upstream 100 bases:

>100_bases
GCACTTCCTTGGTAAGGAAGAGGTCCCGGGTTCAAGTCCCGGTAACAGCTCATAAAGATGTAAATGCTGATTATTAATCA
AATAAATAACAAGTAAAGCT

Downstream 100 bases:

>100_bases
TACACAATAATTAATCAGTTCCCGGTTTTACTATCGGGAACTGATTATGTACAAACGGGAGTAGCTCAGTTGGTAGAGCA
CCGGTCTCCAAAACCGGGTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSF
YDFDGDNTPIIQGSALGALNGVEKWEDKVMELMDAVDNWIPLPPRDVDKPFLMPVEDVFSITGRGTVATGRIESGIIHVG
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGVDKNEIKRGMVLCKPGQIKPHSRFKAEVYILKKEEGGRH
TPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNPGLRFAIREGGRTVGAGQITEIID

Sequences:

>Translated_394_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSF
YDFDGDNTPIIQGSALGALNGVEKWEDKVMELMDAVDNWIPLPPRDVDKPFLMPVEDVFSITGRGTVATGRIESGIIHVG
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGVDKNEIKRGMVLCKPGQIKPHSRFKAEVYILKKEEGGRH
TPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNPGLRFAIREGGRTVGAGQITEIID
>Mature_393_residues
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERGITINTSHVEYETANRHYAHVD
CPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHILLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFY
DFDGDNTPIIQGSALGALNGVEKWEDKVMELMDAVDNWIPLPPRDVDKPFLMPVEDVFSITGRGTVATGRIESGIIHVGD
EVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGVDKNEIKRGMVLCKPGQIKPHSRFKAEVYILKKEEGGRHT
PFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTITVELIYPVALNPGLRFAIREGGRTVGAGQITEIID

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.0913705583756, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=31.4220183486239, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=31.4220183486239, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=27.9642058165548, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=27.6018099547511, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI54607086, Length=317, Percent_Identity=31.8611987381703, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=28.695652173913, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=29.2753623188406, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=29.2753623188406, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=345, Percent_Identity=27.8260869565217, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=27.7153558052434, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=36.0902255639098, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=73.0964467005076, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=73.2824427480916, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=29.6875, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI48994988, Length=282, Percent_Identity=30.8510638297872, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=28.0487804878049, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788922, Length=335, Percent_Identity=26.5671641791045, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.1278195488722, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.9612403100775, Blast_Score=340, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=355, Percent_Identity=31.5492957746479, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=355, Percent_Identity=31.5492957746479, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17506081, Length=290, Percent_Identity=30.3448275862069, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.4599542334096, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=27.4599542334096, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=341, Percent_Identity=30.4985337243402, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=26.2222222222222, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17509919, Length=280, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=27.2340425531915, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.3939393939394, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=458, Percent_Identity=24.235807860262, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=283, Percent_Identity=30.0353356890459, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.4518828451883, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.234096692112, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.234096692112, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=49.1048593350384, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=29.1111111111111, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=29.1111111111111, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=29.1111111111111, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=29.1111111111111, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.0623608017817, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.0623608017817, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45550900, Length=354, Percent_Identity=28.2485875706215, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=349, Percent_Identity=26.9340974212034, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28574573, Length=158, Percent_Identity=32.2784810126582, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=35.1190476190476, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=30.8176100628931, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43636; Mature: 43504

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFYDFDGDNTPIIQGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHC
GVEKWEDKVMELMDAVDNWIPLPPRDVDKPFLMPVEDVFSITGRGTVATGRIESGIIHVG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEEC
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGVDKNEIKRGMVLCKPGQIK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCCC
PHSRFKAEVYILKKEEGGRHTPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTIT
CCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEE
VELIYPVALNPGLRFAIREGGRTVGAGQITEIID
EEEEEEEECCCCCEEEEECCCCEECCCCHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGLSELRSFDSIDNAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGAIIVCAATDGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPRLVVFLNKCDMVDDEEMLELVEMEMRELLSFYDFDGDNTPIIQGSALGALN
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHC
GVEKWEDKVMELMDAVDNWIPLPPRDVDKPFLMPVEDVFSITGRGTVATGRIESGIIHVG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEEC
DEVEILGLGEDKKSVVTGVEMFRKLLDQGEAGDNVGLLLRGVDKNEIKRGMVLCKPGQIK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCCCCC
PHSRFKAEVYILKKEEGGRHTPFHNKYRPQFYLRTMDCTGEITLPEGTEMVMPGDNVTIT
CCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEE
VELIYPVALNPGLRFAIREGGRTVGAGQITEIID
EEEEEEEECCCCCEEEEECCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA