Click here to switch to the map view.

The map label for this gene is fusA [H]

Identifier: 29348138

GI number: 29348138

Start: 3378768

End: 3380885

Strand: Reverse

Name: fusA [H]

Synonym: BT_2729

Alternate gene names: 29348138

Gene position: 3380885-3378768 (Counterclockwise)

Preceding gene: 29348139

Following gene: 29348137

Centisome position: 54.0

GC content: 41.6

Gene sequence:

>2118_bases
ATGGCAAAGACAGATTTACATTTGACTCGTAATATCGGTATCATGGCTCACATTGATGCCGGAAAAACAACAACTTCTGA
ACGTATCCTGTTCTACACTGGATTGACTCACAAAATTGGTGAGGTACACGATGGTGCTGCAACAATGGACTGGATGGAAC
AGGAGCAAGAACGTGGTATTACTATTACATCTGCTGCAACAACAACAAGATGGAAATATGCTGGTGATACTTATAAAATC
AACTTGATTGACACTCCGGGACACGTTGACTTTACTGCTGAAGTAGAACGTTCGTTACGTATTCTGGATGGTGCTGTGGC
TGCTTACTGTGCAGTAGGTGGTGTAGAGCCTCAATCAGAAACTGTATGGCGTCAAGCTGATAAGTATAATGTGCCACGTA
TTGCGTACGTAAACAAAATGGACCGTTCGGGTGCTGACTTCTTTGAGGTTGTACGCCAGATGAAAGCTGTTTTGGGTGCT
AATCCTTGTCCAATCGTTGTTCCTATTGGCGCTGAGGAAACCTTCAAAGGTCTAGTAGACTTGATTAAAATGAAAGCTAT
CTATTGGCATGATGAAACAATGGGGGCTGACTATACAGTTGAAGAAATACCGGCAGATCTCGTTGATGAAGCAAATGAAT
GGAGAGATAAAATGCTTGAAAAGGTAGCTGAGTTTGACGATGCTTTGATGGAGAAATATTTCGACGATCCTTCTACTATT
ACAGAAGAAGAAGTGTTGAGAGCTCTTCGCAATGCAACAGTTCAGATGGCAGTTGTACCAATGTTGTGTGGCTCTTCATT
TAAGAATAAGGGTGTTCAGACATTGTTGGACTATGTTTGTGCTTTCTTGCCTTCTCCATTGGATACTGAGAATGTGATTG
GTACAAATCCGAATACAGGTGCAGAAGAAGATCGTAAACCGAGTGATGATGAGAAAACATCAGCTTTGGCATTTAAGATT
GCTACTGACCCTTATGTAGGTCGTTTGACTTTCTTCCGTGTATATTCGGGTAAGATTGAAGCTGGTTCTTATATCTATAA
TTCTCGCTCTGGTAAGAAAGAACGTGTTTCTCGTCTGTTCCAGATGCATTCAAACAAGCAGAATCCGGTTGAAGTAATCG
GTGCTGGTGATATTGGTGCAGGTGTAGGTTTTAAAGATATTCATACTGGTGATACGCTTTGTGATGAAACAGCTCCGATC
GTATTAGAGTCTATGGACTTCCCAGAACCGGTAATCGGTATTGCAGTTGAGCCTAAAACTCAGAAAGACATGGATAAATT
GTCTAACGGTTTGGCTAAGCTCGCTGAAGAAGATCCGACATTTACAGTTAAGACTGATGAGCAAACAGGACAGACTGTAA
TCTCTGGTATGGGTGAGTTGCACTTGGATATCATTATTGACCGTTTGAAACGTGAATTTAAAGTAGAATGTAACCAAGGT
AAACCTCAGGTTAATTATAAGGAAGCTATCACTAAGACAGTTGATCTTCGTGAAGTTTATAAGAAACAGTCTGGTGGTCG
TGGTAAGTTTGCAGATATTATTGTTAAGATCGGTCCTGTTGATGAAGATTTCAAAGAAGGCGGATTGCAGTTTATCGATG
AAGTGAAGGGTGGTAATATTCCTAAAGAATTTATTCCTTCAGTTCAGAAAGGTTTCACAAGCGCAATGAAGAACGGTGTG
TTGGCCGGATATCCGTTGGATTCAATGAAAGTAACTTTGATTGATGGTTCATTCCACCCGGTTGACTCTGACCAGTTGTC
TTTCGAAATCTGTGCTATTCAGGCATATAAGAATGCATGTGCTAAAGCAGGTCCGGTATTGATGGAGCCTATCATGAAAC
TGGAGGTTGTTACTCCGGAAGAAAACATGGGTGATGTGATCGGTGACTTGAATAAGCGTCGTGGCCAGGTTGAAGGTATG
GAATCTAGCCGTTCCGGTGCTCGTATCGTGAAAGCAATGGTGCCACTGGCTGAAATGTTTGGTTATGTAACAGCGTTACG
TACTATTACATCAGGTCGCGCAACTTCATCAATGGTATATTCTCATCACGCTCAAGTTTCTAACTCTATTGCTAAGGCAG
TATTGGAAGAAGTTAAAGGACGTGCTGATTTACTCTAG

Upstream 100 bases:

>100_bases
AGAATGGCTGAAGCTAACCGTGCATTTGCCCATTTCAGATTCTAATACTATAAGAATATAACCGATTTAGATTAAAAACT
TAAAGTAAAGGAATAAGAAA

Downstream 100 bases:

>100_bases
AAGAATATCAAAAGCAGCAGGCTAGCGAATGACTCTTAGCCTGCCGCTTTATCTTATTTATAAATATATTAATTTAAAAC
CATAATGAGTCAGAAAATTA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MAKTDLHLTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHDGAATMDWMEQEQERGITITSAATTTRWKYAGDTYKI
NLIDTPGHVDFTAEVERSLRILDGAVAAYCAVGGVEPQSETVWRQADKYNVPRIAYVNKMDRSGADFFEVVRQMKAVLGA
NPCPIVVPIGAEETFKGLVDLIKMKAIYWHDETMGADYTVEEIPADLVDEANEWRDKMLEKVAEFDDALMEKYFDDPSTI
TEEEVLRALRNATVQMAVVPMLCGSSFKNKGVQTLLDYVCAFLPSPLDTENVIGTNPNTGAEEDRKPSDDEKTSALAFKI
ATDPYVGRLTFFRVYSGKIEAGSYIYNSRSGKKERVSRLFQMHSNKQNPVEVIGAGDIGAGVGFKDIHTGDTLCDETAPI
VLESMDFPEPVIGIAVEPKTQKDMDKLSNGLAKLAEEDPTFTVKTDEQTGQTVISGMGELHLDIIIDRLKREFKVECNQG
KPQVNYKEAITKTVDLREVYKKQSGGRGKFADIIVKIGPVDEDFKEGGLQFIDEVKGGNIPKEFIPSVQKGFTSAMKNGV
LAGYPLDSMKVTLIDGSFHPVDSDQLSFEICAIQAYKNACAKAGPVLMEPIMKLEVVTPEENMGDVIGDLNKRRGQVEGM
ESSRSGARIVKAMVPLAEMFGYVTALRTITSGRATSSMVYSHHAQVSNSIAKAVLEEVKGRADLL

Sequences:

>Translated_705_residues
MAKTDLHLTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHDGAATMDWMEQEQERGITITSAATTTRWKYAGDTYKI
NLIDTPGHVDFTAEVERSLRILDGAVAAYCAVGGVEPQSETVWRQADKYNVPRIAYVNKMDRSGADFFEVVRQMKAVLGA
NPCPIVVPIGAEETFKGLVDLIKMKAIYWHDETMGADYTVEEIPADLVDEANEWRDKMLEKVAEFDDALMEKYFDDPSTI
TEEEVLRALRNATVQMAVVPMLCGSSFKNKGVQTLLDYVCAFLPSPLDTENVIGTNPNTGAEEDRKPSDDEKTSALAFKI
ATDPYVGRLTFFRVYSGKIEAGSYIYNSRSGKKERVSRLFQMHSNKQNPVEVIGAGDIGAGVGFKDIHTGDTLCDETAPI
VLESMDFPEPVIGIAVEPKTQKDMDKLSNGLAKLAEEDPTFTVKTDEQTGQTVISGMGELHLDIIIDRLKREFKVECNQG
KPQVNYKEAITKTVDLREVYKKQSGGRGKFADIIVKIGPVDEDFKEGGLQFIDEVKGGNIPKEFIPSVQKGFTSAMKNGV
LAGYPLDSMKVTLIDGSFHPVDSDQLSFEICAIQAYKNACAKAGPVLMEPIMKLEVVTPEENMGDVIGDLNKRRGQVEGM
ESSRSGARIVKAMVPLAEMFGYVTALRTITSGRATSSMVYSHHAQVSNSIAKAVLEEVKGRADLL
>Mature_704_residues
AKTDLHLTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHDGAATMDWMEQEQERGITITSAATTTRWKYAGDTYKIN
LIDTPGHVDFTAEVERSLRILDGAVAAYCAVGGVEPQSETVWRQADKYNVPRIAYVNKMDRSGADFFEVVRQMKAVLGAN
PCPIVVPIGAEETFKGLVDLIKMKAIYWHDETMGADYTVEEIPADLVDEANEWRDKMLEKVAEFDDALMEKYFDDPSTIT
EEEVLRALRNATVQMAVVPMLCGSSFKNKGVQTLLDYVCAFLPSPLDTENVIGTNPNTGAEEDRKPSDDEKTSALAFKIA
TDPYVGRLTFFRVYSGKIEAGSYIYNSRSGKKERVSRLFQMHSNKQNPVEVIGAGDIGAGVGFKDIHTGDTLCDETAPIV
LESMDFPEPVIGIAVEPKTQKDMDKLSNGLAKLAEEDPTFTVKTDEQTGQTVISGMGELHLDIIIDRLKREFKVECNQGK
PQVNYKEAITKTVDLREVYKKQSGGRGKFADIIVKIGPVDEDFKEGGLQFIDEVKGGNIPKEFIPSVQKGFTSAMKNGVL
AGYPLDSMKVTLIDGSFHPVDSDQLSFEICAIQAYKNACAKAGPVLMEPIMKLEVVTPEENMGDVIGDLNKRRGQVEGME
SSRSGARIVKAMVPLAEMFGYVTALRTITSGRATSSMVYSHHAQVSNSIAKAVLEEVKGRADLL

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=687, Percent_Identity=43.8136826783115, Blast_Score=535, Evalue=1e-152,
Organism=Homo sapiens, GI19923640, Length=718, Percent_Identity=37.883008356546, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI25306287, Length=718, Percent_Identity=34.1225626740947, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI25306283, Length=456, Percent_Identity=41.0087719298246, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI4503483, Length=482, Percent_Identity=26.3485477178423, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=41.3533834586466, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=34.1772151898734, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=41.025641025641, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=41.025641025641, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=41.025641025641, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966752, Length=95, Percent_Identity=40, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=57.1022727272727, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=498, Percent_Identity=28.1124497991968, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788922, Length=177, Percent_Identity=37.2881355932203, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=39.7058823529412, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=684, Percent_Identity=40.4970760233918, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=710, Percent_Identity=27.4647887323944, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17506493, Length=813, Percent_Identity=26.5682656826568, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=41.5584415584416, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=159, Percent_Identity=30.8176100628931, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=159, Percent_Identity=30.8176100628931, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=695, Percent_Identity=40, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322359, Length=802, Percent_Identity=32.2942643391521, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324707, Length=489, Percent_Identity=26.7893660531697, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320593, Length=489, Percent_Identity=26.7893660531697, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=179, Percent_Identity=31.2849162011173, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582462, Length=689, Percent_Identity=42.9608127721335, Blast_Score=530, Evalue=1e-150,
Organism=Drosophila melanogaster, GI221458488, Length=728, Percent_Identity=32.6923076923077, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24585711, Length=495, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24585713, Length=495, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24585709, Length=495, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI78706572, Length=153, Percent_Identity=36.6013071895425, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI28574573, Length=167, Percent_Identity=34.1317365269461, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77588; Mature: 77457

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTDLHLTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHDGAATMDWMEQEQERGI
CCCCCEEEEECCCEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHCCE
TITSAATTTRWKYAGDTYKINLIDTPGHVDFTAEVERSLRILDGAVAAYCAVGGVEPQSE
EEEECCCCCEEEECCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TVWRQADKYNVPRIAYVNKMDRSGADFFEVVRQMKAVLGANPCPIVVPIGAEETFKGLVD
HHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHH
LIKMKAIYWHDETMGADYTVEEIPADLVDEANEWRDKMLEKVAEFDDALMEKYFDDPSTI
HHHHHHHEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TEEEVLRALRNATVQMAVVPMLCGSSFKNKGVQTLLDYVCAFLPSPLDTENVIGTNPNTG
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEEDRKPSDDEKTSALAFKIATDPYVGRLTFFRVYSGKIEAGSYIYNSRSGKKERVSRLF
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHH
QMHSNKQNPVEVIGAGDIGAGVGFKDIHTGDTLCDETAPIVLESMDFPEPVIGIAVEPKT
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCEEEECCCCCCCCEEEEECCCC
QKDMDKLSNGLAKLAEEDPTFTVKTDEQTGQTVISGMGELHLDIIIDRLKREFKVECNQG
HHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCC
KPQVNYKEAITKTVDLREVYKKQSGGRGKFADIIVKIGPVDEDFKEGGLQFIDEVKGGNI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHCHHHHHHHHCCCCC
PKEFIPSVQKGFTSAMKNGVLAGYPLDSMKVTLIDGSFHPVDSDQLSFEICAIQAYKNAC
CHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHH
AKAGPVLMEPIMKLEVVTPEENMGDVIGDLNKRRGQVEGMESSRSGARIVKAMVPLAEMF
HHCCCHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
GYVTALRTITSGRATSSMVYSHHAQVSNSIAKAVLEEVKGRADLL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
AKTDLHLTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHDGAATMDWMEQEQERGI
CCCCEEEEECCCEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHCCE
TITSAATTTRWKYAGDTYKINLIDTPGHVDFTAEVERSLRILDGAVAAYCAVGGVEPQSE
EEEECCCCCEEEECCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TVWRQADKYNVPRIAYVNKMDRSGADFFEVVRQMKAVLGANPCPIVVPIGAEETFKGLVD
HHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHH
LIKMKAIYWHDETMGADYTVEEIPADLVDEANEWRDKMLEKVAEFDDALMEKYFDDPSTI
HHHHHHHEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TEEEVLRALRNATVQMAVVPMLCGSSFKNKGVQTLLDYVCAFLPSPLDTENVIGTNPNTG
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEEDRKPSDDEKTSALAFKIATDPYVGRLTFFRVYSGKIEAGSYIYNSRSGKKERVSRLF
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHH
QMHSNKQNPVEVIGAGDIGAGVGFKDIHTGDTLCDETAPIVLESMDFPEPVIGIAVEPKT
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCEEEECCCCCCCCEEEEECCCC
QKDMDKLSNGLAKLAEEDPTFTVKTDEQTGQTVISGMGELHLDIIIDRLKREFKVECNQG
HHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCC
KPQVNYKEAITKTVDLREVYKKQSGGRGKFADIIVKIGPVDEDFKEGGLQFIDEVKGGNI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHCHHHHHHHHCCCCC
PKEFIPSVQKGFTSAMKNGVLAGYPLDSMKVTLIDGSFHPVDSDQLSFEICAIQAYKNAC
CHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHH
AKAGPVLMEPIMKLEVVTPEENMGDVIGDLNKRRGQVEGMESSRSGARIVKAMVPLAEMF
HHCCCHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
GYVTALRTITSGRATSSMVYSHHAQVSNSIAKAVLEEVKGRADLL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA