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The map label for this gene is ykfA [H]

Identifier: 29347959

GI number: 29347959

Start: 3181823

End: 3182731

Strand: Reverse

Name: ykfA [H]

Synonym: BT_2549

Alternate gene names: 29347959

Gene position: 3182731-3181823 (Counterclockwise)

Preceding gene: 29347960

Following gene: 29347958

Centisome position: 50.84

GC content: 47.08

Gene sequence:

>909_bases
ATGAATATTCAACTTCCTTCTTTTTTACAGAAAGGTGACAAAGTAGTGATTGTGTCACCTTCCAGCAAGATAGACAAAGA
GTTTCTGAAACGTGCGAAGAAACGGCTTGAATCATGGGGATTGAAAGTCTCTGTGGGGAAATATGCCGGTGGCTCTTCCG
GACGTTATGCCGGAACGGTCCGGCAACGTCTGCAAGACTTGCAGAGTGCGATGGATGATCCTAAAGTGAAAGCTATTCTT
TGTAGCAGAGGAGGATACGGGGCCGTGCATCTGGTGGACAAGATTGACTTCACCGCCTTTCGTGAACATCCGAAGTGGTT
GCTGGGATTCAGCGACATCACTGCCTTGCATAATTTATTTCAGAAGAATGGATATGCCTCCCTGCATTCGCTGATGGCGC
GTCATCTGAGCGTGGAGCCGGAAGAAGACCCTTGCGTGGCCTATCTGAAAGATATTCTCTTCGGTAATCTGCCCGTTTAT
ACTTGCGAAAAGCATAAACTGAACAGGCAAGGTACGGCCGAAGGTATCCTGCGTGGCGGAAATATGGCGGTAGCTTACGG
GCTGCGCGGCACTCCTTATGATATTCCTGCCGAAGGAACCATCCTGTTTCTCGAAGATGTCAGTGAACGTCCCCATGCGA
TTGAGCGCATGATGTACAATCTGAAACTGGGCGGTGTGCTTGAAAAACTCTCCGGACTCATTATCGGTCAATTTACCGAG
TATGAAGAAGATTGTTCGTTGGGTAAGGAGTTGTATCCCGCTCTGGCCGACTTGGTGAAAGAATATGACTATCCGGTTTG
TTTTAATTTCCCGGTAGGTCATGTGACGCATAATTTACCGCTTATCAATGGCGCGAAAGTGGAATTGACCGTTGGAAAGA
AAAATGTGGAGTTAAAGTTTATTTGTTAA

Upstream 100 bases:

>100_bases
TCGTGGGTCGGAGTAGTCGATCCCAAAGACCTTCCGGTAGAAATGGAAGTGGACTGGGTGCGTTTTTATCAGCGAAAGTC
ACTCAAATAAAACATTAAAT

Downstream 100 bases:

>100_bases
TAATCCATTCTATCTGAACGATACTGTGGTGAAAATGACTAATTTTGAGGCTTCATATAGATAAAAGGACGATGAAAAAG
AAATCTATGATAGTGTTGAC

Product: putative carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNIQLPSFLQKGDKVVIVSPSSKIDKEFLKRAKKRLESWGLKVSVGKYAGGSSGRYAGTVRQRLQDLQSAMDDPKVKAIL
CSRGGYGAVHLVDKIDFTAFREHPKWLLGFSDITALHNLFQKNGYASLHSLMARHLSVEPEEDPCVAYLKDILFGNLPVY
TCEKHKLNRQGTAEGILRGGNMAVAYGLRGTPYDIPAEGTILFLEDVSERPHAIERMMYNLKLGGVLEKLSGLIIGQFTE
YEEDCSLGKELYPALADLVKEYDYPVCFNFPVGHVTHNLPLINGAKVELTVGKKNVELKFIC

Sequences:

>Translated_302_residues
MNIQLPSFLQKGDKVVIVSPSSKIDKEFLKRAKKRLESWGLKVSVGKYAGGSSGRYAGTVRQRLQDLQSAMDDPKVKAIL
CSRGGYGAVHLVDKIDFTAFREHPKWLLGFSDITALHNLFQKNGYASLHSLMARHLSVEPEEDPCVAYLKDILFGNLPVY
TCEKHKLNRQGTAEGILRGGNMAVAYGLRGTPYDIPAEGTILFLEDVSERPHAIERMMYNLKLGGVLEKLSGLIIGQFTE
YEEDCSLGKELYPALADLVKEYDYPVCFNFPVGHVTHNLPLINGAKVELTVGKKNVELKFIC
>Mature_302_residues
MNIQLPSFLQKGDKVVIVSPSSKIDKEFLKRAKKRLESWGLKVSVGKYAGGSSGRYAGTVRQRLQDLQSAMDDPKVKAIL
CSRGGYGAVHLVDKIDFTAFREHPKWLLGFSDITALHNLFQKNGYASLHSLMARHLSVEPEEDPCVAYLKDILFGNLPVY
TCEKHKLNRQGTAEGILRGGNMAVAYGLRGTPYDIPAEGTILFLEDVSERPHAIERMMYNLKLGGVLEKLSGLIIGQFTE
YEEDCSLGKELYPALADLVKEYDYPVCFNFPVGHVTHNLPLINGAKVELTVGKKNVELKFIC

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=299, Percent_Identity=28.7625418060201, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33606; Mature: 33606

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQLPSFLQKGDKVVIVSPSSKIDKEFLKRAKKRLESWGLKVSVGKYAGGSSGRYAGTV
CCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
RQRLQDLQSAMDDPKVKAILCSRGGYGAVHLVDKIDFTAFREHPKWLLGFSDITALHNLF
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCHHHHHCCCCEEECHHHHHHHHHHH
QKNGYASLHSLMARHLSVEPEEDPCVAYLKDILFGNLPVYTCEKHKLNRQGTAEGILRGG
HCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHCCCCCCHHHHEECC
NMAVAYGLRGTPYDIPAEGTILFLEDVSERPHAIERMMYNLKLGGVLEKLSGLIIGQFTE
CEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YEEDCSLGKELYPALADLVKEYDYPVCFNFPVGHVTHNLPLINGAKVELTVGKKNVELKF
CHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCEECCCEEEEEECCCCEEEEE
IC
EC
>Mature Secondary Structure
MNIQLPSFLQKGDKVVIVSPSSKIDKEFLKRAKKRLESWGLKVSVGKYAGGSSGRYAGTV
CCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
RQRLQDLQSAMDDPKVKAILCSRGGYGAVHLVDKIDFTAFREHPKWLLGFSDITALHNLF
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCHHHHHCCCCEEECHHHHHHHHHHH
QKNGYASLHSLMARHLSVEPEEDPCVAYLKDILFGNLPVYTCEKHKLNRQGTAEGILRGG
HCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHCCCCCCHHHHEECC
NMAVAYGLRGTPYDIPAEGTILFLEDVSERPHAIERMMYNLKLGGVLEKLSGLIIGQFTE
CEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YEEDCSLGKELYPALADLVKEYDYPVCFNFPVGHVTHNLPLINGAKVELTVGKKNVELKF
CHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCEECCCEEEEEECCCCEEEEE
IC
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]