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The map label for this gene is ispG

Identifier: 29347927

GI number: 29347927

Start: 3141356

End: 3143197

Strand: Reverse

Name: ispG

Synonym: BT_2517

Alternate gene names: 29347927

Gene position: 3143197-3141356 (Counterclockwise)

Preceding gene: 29347928

Following gene: 29347925

Centisome position: 50.21

GC content: 49.89

Gene sequence:

>1842_bases
ATGGATTTATTCAATTACTTTCGAAGAGAAACATCCGAAGTAAATATTGGGGCTGTACCTTTGGGCGGCCCCAATCCTAT
TCGTATCCAGTCGATGACGAACACGTCCACTATGGATACAGAAGCTTGTGTGGAACAAGCCAAACGCATCGTCGATGCCG
GCGGCGAGTATGTACGACTGACCACGCAGGGTGTCAAGGAAGCGGAAAATCTGATGAATATCAATATCGGGCTACGAAGT
CAGGGATATATGGTTCCGTTGGTGGCTGATGTGCATTTTAATCCGAAAGTGGCCGATGTAGCAGCTCAATATGCAGAAAA
GGTACGGATCAATCCGGGCAATTATGTAGATGCAGCGCGTACTTTTAAGAAACTGGAATATACCGACGAGGAGTATGCGC
AGGAGATACAGAAGATTCACGACCGTTTTGTGCCGTTTCTGAATATCTGTAAGGAGAATCATACGGCTATCCGCATCGGA
GTCAATCACGGTTCCCTGTCCGACCGTATCATGTCCCGCTACGGAGACACTCCCGAAGGGATGGTGGAATCCTGTATGGA
ATTCCTGCGTATCTGTGTGGCGGAACATTTCACGGATGTGGTGATTTCGATCAAGGCTTCCAATACGGTGGTGATGGTGA
AGACCGTCCGCTTGCTTGTTGCCGTGATGGAACAGGAGGGAATGTCATTTCCCCTGCATCTTGGAGTGACGGAGGCCGGA
GACGGAGAAGACGGACGTATAAAATCTGCCTTGGGCATCGGTGCATTGCTTTGTGACGGATTGGGAGATACGATTCGTGT
GTCGCTTAGTGAGGCTCCCGAAGCGGAAATTCCCGTAGCGCGCAAGCTGGTGGATTATGTTCTGTTGAGGCAGGATCATC
CGTATATCCCGGGTATGGAGGCACCGGAATTTAATTATCTTTCTCCTTCGCGCCGCAAGACGCGGGCAGTGCGGAATATC
GGGGGCGAACATCTCCCTGTCGTTATTGCCGACCGCATGGATGGGAAGACGGAAGTCAATCCGCAGTTTACTCCGGATTA
TATCTATGCCGGAAGAACGTTGCCCGAACAGCGGGAAGAAGGAGTGGAATATATCCTTGATGCCGATGTATGGGAAGGGG
AGGCCGGAACATGGCCTGCTTTCAATCATGCGCAGTTGCCGTTGATGGGCGAGTGCAGTGCAGAACTGAAATTCCTGTTT
ATGCCTTACATGGCGCAAACGGATGAAGTGATTGCCTGTCTTAAGGTGCATCCCGAAGTTGTGGTTATTTCTCAGAGCAA
TCATCCGAATCGCCTGGGCGAGCACCGTGCATTGGTGCATCAACTTATGACGGAAGGTCTGGAGAATCCGGTTGTTTTCT
TCCAGCATTATGCGGAGGACGAGGCGGAAGATTTGCAGATTAAGTCCGCCGTTGATATGGGAGCATTGATTTTTGACGGT
TTATGTGACGGGATTTTCCTGTTCAATCAAGGAAGTCTGAGTCATGCGGTAGTAGATGCTACTGCCTTCGGTATCTTGCA
GGCAGGACGCACCCGTACTTCTAAAACGGAATATATTTCTTGTCCGGGCTGCGGTCGTACGCTTTATGATCTGGAAAAGA
CAATTGCGCGTATCAAGGCGGCTACCTCACATCTGAAAGGCTTGAAAATCGGCATCATGGGATGTATCGTCAACGGTCCC
GGTGAGATGGCGGATGCCGACTACGGCTATGTAGGTGCCGGACGCGGAAAAATCAGCCTTTATAAAGGTAAAGTTTGCGT
AGAGAAAAACATACCCGAAGAAGAGGCGGTGGAACGGTTGCTGGAATTTATCCGCAATGATCGGAAAGAACTGGAACTTT
AA

Upstream 100 bases:

>100_bases
GGCTGAGGCTTTTGCTGCTTACAAAGAAGGACTGAAAAAGAAAATCGTAAAGGCAAACGAAGAACTGAAAGAAGTGAAGT
TCGAATATAAAACCAATTAA

Downstream 100 bases:

>100_bases
TCAGTTATACTTTTAAATAGTTAAGACAGGAGTTGCTTTAAGTTTGCTTAAGGCAACTCCTGTCTTTTATTATAGTATAC
ACAAACATTTATCCGTACAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MDLFNYFRRETSEVNIGAVPLGGPNPIRIQSMTNTSTMDTEACVEQAKRIVDAGGEYVRLTTQGVKEAENLMNINIGLRS
QGYMVPLVADVHFNPKVADVAAQYAEKVRINPGNYVDAARTFKKLEYTDEEYAQEIQKIHDRFVPFLNICKENHTAIRIG
VNHGSLSDRIMSRYGDTPEGMVESCMEFLRICVAEHFTDVVISIKASNTVVMVKTVRLLVAVMEQEGMSFPLHLGVTEAG
DGEDGRIKSALGIGALLCDGLGDTIRVSLSEAPEAEIPVARKLVDYVLLRQDHPYIPGMEAPEFNYLSPSRRKTRAVRNI
GGEHLPVVIADRMDGKTEVNPQFTPDYIYAGRTLPEQREEGVEYILDADVWEGEAGTWPAFNHAQLPLMGECSAELKFLF
MPYMAQTDEVIACLKVHPEVVVISQSNHPNRLGEHRALVHQLMTEGLENPVVFFQHYAEDEAEDLQIKSAVDMGALIFDG
LCDGIFLFNQGSLSHAVVDATAFGILQAGRTRTSKTEYISCPGCGRTLYDLEKTIARIKAATSHLKGLKIGIMGCIVNGP
GEMADADYGYVGAGRGKISLYKGKVCVEKNIPEEEAVERLLEFIRNDRKELEL

Sequences:

>Translated_613_residues
MDLFNYFRRETSEVNIGAVPLGGPNPIRIQSMTNTSTMDTEACVEQAKRIVDAGGEYVRLTTQGVKEAENLMNINIGLRS
QGYMVPLVADVHFNPKVADVAAQYAEKVRINPGNYVDAARTFKKLEYTDEEYAQEIQKIHDRFVPFLNICKENHTAIRIG
VNHGSLSDRIMSRYGDTPEGMVESCMEFLRICVAEHFTDVVISIKASNTVVMVKTVRLLVAVMEQEGMSFPLHLGVTEAG
DGEDGRIKSALGIGALLCDGLGDTIRVSLSEAPEAEIPVARKLVDYVLLRQDHPYIPGMEAPEFNYLSPSRRKTRAVRNI
GGEHLPVVIADRMDGKTEVNPQFTPDYIYAGRTLPEQREEGVEYILDADVWEGEAGTWPAFNHAQLPLMGECSAELKFLF
MPYMAQTDEVIACLKVHPEVVVISQSNHPNRLGEHRALVHQLMTEGLENPVVFFQHYAEDEAEDLQIKSAVDMGALIFDG
LCDGIFLFNQGSLSHAVVDATAFGILQAGRTRTSKTEYISCPGCGRTLYDLEKTIARIKAATSHLKGLKIGIMGCIVNGP
GEMADADYGYVGAGRGKISLYKGKVCVEKNIPEEEAVERLLEFIRNDRKELEL
>Mature_613_residues
MDLFNYFRRETSEVNIGAVPLGGPNPIRIQSMTNTSTMDTEACVEQAKRIVDAGGEYVRLTTQGVKEAENLMNINIGLRS
QGYMVPLVADVHFNPKVADVAAQYAEKVRINPGNYVDAARTFKKLEYTDEEYAQEIQKIHDRFVPFLNICKENHTAIRIG
VNHGSLSDRIMSRYGDTPEGMVESCMEFLRICVAEHFTDVVISIKASNTVVMVKTVRLLVAVMEQEGMSFPLHLGVTEAG
DGEDGRIKSALGIGALLCDGLGDTIRVSLSEAPEAEIPVARKLVDYVLLRQDHPYIPGMEAPEFNYLSPSRRKTRAVRNI
GGEHLPVVIADRMDGKTEVNPQFTPDYIYAGRTLPEQREEGVEYILDADVWEGEAGTWPAFNHAQLPLMGECSAELKFLF
MPYMAQTDEVIACLKVHPEVVVISQSNHPNRLGEHRALVHQLMTEGLENPVVFFQHYAEDEAEDLQIKSAVDMGALIFDG
LCDGIFLFNQGSLSHAVVDATAFGILQAGRTRTSKTEYISCPGCGRTLYDLEKTIARIKAATSHLKGLKIGIMGCIVNGP
GEMADADYGYVGAGRGKISLYKGKVCVEKNIPEEEAVERLLEFIRNDRKELEL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=285, Percent_Identity=35.7894736842105, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BACTN (Q8A4T0)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_811430.1
- ProteinModelPortal:   Q8A4T0
- GeneID:   1076351
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_2517
- NMPDR:   fig|226186.1.peg.2517
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   PRK02048
- BioCyc:   BTHE226186:BT_2517-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 67897; Mature: 67897

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFNYFRRETSEVNIGAVPLGGPNPIRIQSMTNTSTMDTEACVEQAKRIVDAGGEYVRL
CCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTQGVKEAENLMNINIGLRSQGYMVPLVADVHFNPKVADVAAQYAEKVRINPGNYVDAAR
EHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHEECCCCCCHHHHH
TFKKLEYTDEEYAQEIQKIHDRFVPFLNICKENHTAIRIGVNHGSLSDRIMSRYGDTPEG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHH
MVESCMEFLRICVAEHFTDVVISIKASNTVVMVKTVRLLVAVMEQEGMSFPLHLGVTEAG
HHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCC
DGEDGRIKSALGIGALLCDGLGDTIRVSLSEAPEAEIPVARKLVDYVLLRQDHPYIPGME
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
APEFNYLSPSRRKTRAVRNIGGEHLPVVIADRMDGKTEVNPQFTPDYIYAGRTLPEQREE
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHC
GVEYILDADVWEGEAGTWPAFNHAQLPLMGECSAELKFLFMPYMAQTDEVIACLKVHPEV
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHEECCCCE
VVISQSNHPNRLGEHRALVHQLMTEGLENPVVFFQHYAEDEAEDLQIKSAVDMGALIFDG
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHEEHHHHHHHHHHHHHH
LCDGIFLFNQGSLSHAVVDATAFGILQAGRTRTSKTEYISCPGCGRTLYDLEKTIARIKA
HHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
ATSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGKISLYKGKVCVEKNIPEEEAVERL
HHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEECCEEEECCCCHHHHHHHH
LEFIRNDRKELEL
HHHHHCCHHHCCC
>Mature Secondary Structure
MDLFNYFRRETSEVNIGAVPLGGPNPIRIQSMTNTSTMDTEACVEQAKRIVDAGGEYVRL
CCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTQGVKEAENLMNINIGLRSQGYMVPLVADVHFNPKVADVAAQYAEKVRINPGNYVDAAR
EHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHEECCCCCCHHHHH
TFKKLEYTDEEYAQEIQKIHDRFVPFLNICKENHTAIRIGVNHGSLSDRIMSRYGDTPEG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHH
MVESCMEFLRICVAEHFTDVVISIKASNTVVMVKTVRLLVAVMEQEGMSFPLHLGVTEAG
HHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCC
DGEDGRIKSALGIGALLCDGLGDTIRVSLSEAPEAEIPVARKLVDYVLLRQDHPYIPGME
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
APEFNYLSPSRRKTRAVRNIGGEHLPVVIADRMDGKTEVNPQFTPDYIYAGRTLPEQREE
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHC
GVEYILDADVWEGEAGTWPAFNHAQLPLMGECSAELKFLFMPYMAQTDEVIACLKVHPEV
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHEECCCCE
VVISQSNHPNRLGEHRALVHQLMTEGLENPVVFFQHYAEDEAEDLQIKSAVDMGALIFDG
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHEEHHHHHHHHHHHHHH
LCDGIFLFNQGSLSHAVVDATAFGILQAGRTRTSKTEYISCPGCGRTLYDLEKTIARIKA
HHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
ATSHLKGLKIGIMGCIVNGPGEMADADYGYVGAGRGKISLYKGKVCVEKNIPEEEAVERL
HHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEECCEEEECCCCHHHHHHHH
LEFIRNDRKELEL
HHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928