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The map label for this gene is yfkN [H]

Identifier: 29347850

GI number: 29347850

Start: 3056406

End: 3057293

Strand: Reverse

Name: yfkN [H]

Synonym: BT_2440

Alternate gene names: 29347850

Gene position: 3057293-3056406 (Counterclockwise)

Preceding gene: 29347851

Following gene: 29347849

Centisome position: 48.84

GC content: 43.58

Gene sequence:

>888_bases
ATGAAATCTCAGTATCCTATGACAACTCATAATCATATAACTTCCGTCTTGAATATATTCAAAGGAAGAACCGGAAAGTG
GCTGATGCTTTTCCTCCTTTTACTACCTGCATTGGCAACTGCCCAGGAAACAAAAAAACTAATCATACTGCAAACCAGTG
ATGTACACAGCCGGATTGAACCTATGACTCAGGAAGGCGACCGGAACTACGGTCAGGGTGGATTTGTCCGTCGGGCAAGT
TTCCTGCAGCAGTTCCGCAAAGAAAATCCGGATGTACTGCTGTTCGATTGCGGCGATATCTCGCAAGGCACTCCTTATTA
TAATATGTTTAAAGGTGAGGTAGAAGTCACCCTGATGAATGAAATGGGATACGATGCAATGACAATCGGTAACCACGAGT
TCGATTTCGGTTTGGACAACATGGCTCGTCTATTTAAATTAGCCAAGTTTCCGGTAGTATGTGCCAACTATGATCTTGAT
GCCACAGTACTCAAAGATATCGTAAAACCTTATGTCATTCTGGATCGTTTCGGTTTGAAGATCGGAGTTTTCGGGTTGGG
AGCGAAGCCGGAAGGACTGATACAGGCCAATAAATGCGAAGGGGTAATCTATAAAGACCCGATCGCCGTATCCAATGACA
TTGCCGCACAACTGAAAGAAGAAGGATGCGATGTCATTGTATGCCTGTCACATCTGGGTATTCAGATGGATGAGAAGCTG
GTAGCCAACACCCGTAATATCGATGTTATTCTGGGTGGGCATTCTCATACCTTTATGGAAAGCCCCAAGAATTATCTGAA
CATAGACGGGAAAAATGTCCCCGTTATGCACACCGGAAAGAATGGAATTTATGTTGGACGACTAGATTTGACCTTGAATA
AAAAATGA

Upstream 100 bases:

>100_bases
GCAAGAAGATTACCTCTAAACTGGATGGACGTATCACCGTTGTTCCGGCATCCGACCGGAAATAAAAAAATGAAAAGAGA
ATAAATAACCAAGGTTTACA

Downstream 100 bases:

>100_bases
GATTACTAACATATATACTGGCTATCCTGCTATTTCTTCCTGTCAAGGCCAGGGGAGAAGCAATAGATACTCCCGTAGAA
CCTTTACTACTTTATAAAGC

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKSQYPMTTHNHITSVLNIFKGRTGKWLMLFLLLLPALATAQETKKLIILQTSDVHSRIEPMTQEGDRNYGQGGFVRRAS
FLQQFRKENPDVLLFDCGDISQGTPYYNMFKGEVEVTLMNEMGYDAMTIGNHEFDFGLDNMARLFKLAKFPVVCANYDLD
ATVLKDIVKPYVILDRFGLKIGVFGLGAKPEGLIQANKCEGVIYKDPIAVSNDIAAQLKEEGCDVIVCLSHLGIQMDEKL
VANTRNIDVILGGHSHTFMESPKNYLNIDGKNVPVMHTGKNGIYVGRLDLTLNKK

Sequences:

>Translated_295_residues
MKSQYPMTTHNHITSVLNIFKGRTGKWLMLFLLLLPALATAQETKKLIILQTSDVHSRIEPMTQEGDRNYGQGGFVRRAS
FLQQFRKENPDVLLFDCGDISQGTPYYNMFKGEVEVTLMNEMGYDAMTIGNHEFDFGLDNMARLFKLAKFPVVCANYDLD
ATVLKDIVKPYVILDRFGLKIGVFGLGAKPEGLIQANKCEGVIYKDPIAVSNDIAAQLKEEGCDVIVCLSHLGIQMDEKL
VANTRNIDVILGGHSHTFMESPKNYLNIDGKNVPVMHTGKNGIYVGRLDLTLNKK
>Mature_295_residues
MKSQYPMTTHNHITSVLNIFKGRTGKWLMLFLLLLPALATAQETKKLIILQTSDVHSRIEPMTQEGDRNYGQGGFVRRAS
FLQQFRKENPDVLLFDCGDISQGTPYYNMFKGEVEVTLMNEMGYDAMTIGNHEFDFGLDNMARLFKLAKFPVVCANYDLD
ATVLKDIVKPYVILDRFGLKIGVFGLGAKPEGLIQANKCEGVIYKDPIAVSNDIAAQLKEEGCDVIVCLSHLGIQMDEKL
VANTRNIDVILGGHSHTFMESPKNYLNIDGKNVPVMHTGKNGIYVGRLDLTLNKK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=287, Percent_Identity=32.0557491289199, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1790658, Length=304, Percent_Identity=28.9473684210526, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786687, Length=290, Percent_Identity=30, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922444, Length=312, Percent_Identity=27.8846153846154, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28573524, Length=278, Percent_Identity=28.0575539568345, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922446, Length=304, Percent_Identity=26.6447368421053, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24654424, Length=304, Percent_Identity=26.6447368421053, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI221329836, Length=273, Percent_Identity=28.2051282051282, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24641187, Length=273, Percent_Identity=28.2051282051282, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161076508, Length=223, Percent_Identity=31.390134529148, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921980, Length=223, Percent_Identity=31.390134529148, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652512, Length=223, Percent_Identity=31.390134529148, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 32996; Mature: 32996

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQYPMTTHNHITSVLNIFKGRTGKWLMLFLLLLPALATAQETKKLIILQTSDVHSRIE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHC
PMTQEGDRNYGQGGFVRRASFLQQFRKENPDVLLFDCGDISQGTPYYNMFKGEVEVTLMN
CCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECEEEEEEEC
EMGYDAMTIGNHEFDFGLDNMARLFKLAKFPVVCANYDLDATVLKDIVKPYVILDRFGLK
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
IGVFGLGAKPEGLIQANKCEGVIYKDPIAVSNDIAAQLKEEGCDVIVCLSHLGIQMDEKL
EEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEHHCCCCCCHHH
VANTRNIDVILGGHSHTFMESPKNYLNIDGKNVPVMHTGKNGIYVGRLDLTLNKK
HCCCCCEEEEECCCCCHHHHCCCHHEECCCCCCCEEEECCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKSQYPMTTHNHITSVLNIFKGRTGKWLMLFLLLLPALATAQETKKLIILQTSDVHSRIE
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHC
PMTQEGDRNYGQGGFVRRASFLQQFRKENPDVLLFDCGDISQGTPYYNMFKGEVEVTLMN
CCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECEEEEEEEC
EMGYDAMTIGNHEFDFGLDNMARLFKLAKFPVVCANYDLDATVLKDIVKPYVILDRFGLK
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
IGVFGLGAKPEGLIQANKCEGVIYKDPIAVSNDIAAQLKEEGCDVIVCLSHLGIQMDEKL
EEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEHHCCCCCCHHH
VANTRNIDVILGGHSHTFMESPKNYLNIDGKNVPVMHTGKNGIYVGRLDLTLNKK
HCCCCCEEEEECCCCCHHHHCCCHHEECCCCCCCEEEECCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]